Emmanuel M. Khazalwa
Hussein M. Abkallo
Yuan Zhou
Xiongwei Nie
Jinxue Ruan
Changzhi Zhao
Jieru Wang
Jing Xu
Xinyun Li
Shuhong Zhao
Erwei Zuo
Lucilla Steinaa
Shengsong Xie
a Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education & Key Lab of Swine Genetics and Breeding of Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan 430070, PR China;
b Animal and Human Health Program, Biosciences, International Livestock Research Institute (ILRI), P.O. Box 30709, Nairobi 00100, Kenya;
c Key Laboratory of Pig Molecular Quantitative Genetics of Anhui Academy of Agricultural Sciences, Livestock and Poultry Epidemic Diseases Research Center of Anhui Province, Anhui Provincial Key Laboratory of Livestock and Poultry Product Safety Engineering, Institute of Animal Husbandry and Veterinary Medicine, Anhui Academy of Agricultural Sciences, Hefei 230031, PR China;
d The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan 430070, PR China;
e Lingnan Guangdong Laboratory of Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518124, PR China
Funds: This work was supported by the National Transgenic Project of China (2016ZX08006003-004 and 2018ZX08009-26B), the NSFC Major Research Plan-Major Scientific Problems of African Swine Fever virus (31941008), the Fundamental Research Funds for the Central Universities (2662018JC002), the IDRC Livestock Vaccine Innovation Fund (109212-001), the CGIAR Research Program on Livestock, and Natural Science Foundation of Anhui Province (2008085QC138).
Received Date: 2020-12-28
Accepted Date:2021-03-13
Rev Recd Date:2021-03-10
Publish Date:2021-05-20
Abstract
Abstract
Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (CRISPR/Cas9) genome editing technology has dramatically influenced swine research by enabling the production of high-quality disease-resistant pig breeds, thus improving yields. In addition, CRISPR/Cas9 has been used extensively in pigs as one of the tools in biomedical research. In this review, we present the advancements of the CRISPR/Cas9 system in swine research, such as animal breeding, vaccine development, xenotransplantation, and disease modeling. We also highlight the current challenges and some potential applications of the CRISPR/Cas9 technologies.Keywords: CRISPR/Cas9,
Swine,
Genome editing,
CRISPR screening,
Base editing
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