王道文
职称：研究员

职务：

联系电话：86-

传真：

附件：
E-mail：

实验室主页：

研究方向：重要农艺性状的遗传基础和分子改良


王道文，博士，研究员，博士生导师
1993年在英国John Innes Center / University of East Anglia学习并获得博士学位，1997年入选中国科学院"****"并获得国家****科学基金，1997年至今在中国科学院遗传发育所担任课题组长。

王道文博士研究组的主要研究方向是植物分子生物学、重要农艺性状分子机理与遗传改良。研究课题包括小麦籽粒品质和抗病耐逆性状的遗传基础与分子改良以及植物重要功能基因的比较生物学研究。
1. 小麦籽粒终用途和营养品质的遗传基础与改良
小麦籽粒品质性状包括多个方面，但主要是终用途品质和营养功能品质。本组目前的研究主要以三类面筋蛋白及其调控基因为核心，利用功能基因组学技术解析其分子和功能互作网络，为籽粒终用途与营养功能品质的改良提供有益指导和基因资源。同时，我们还在全基因组水平上，系统鉴定小麦脂肪氧化酶编码基因并研究其功能，旨在以其为靶点改良小麦籽粒的营养功能品质与耐储存性。
2. 小麦氮、磷元素利用效率的分子基础与改良
提高农作物对土壤中矿质元素的利用效率对于减少化肥施用量、降低农作物生产成本和保护生态环境具有重要意义。多年的遗传、生理和生化研究表明植物对矿质元素的吸收、同化和利用过程受多个基因的调控。因此，我们主要利用全基因组关联分析、转录组学和转基因等技术手段，鉴定控制小麦氮、磷元素利用效率的染色体位点和基因。在此基础上，通过常规与分子育种方法的运用，培育出具有资源高效利用、优质、高产等性状的小麦新种质和新品种。
3. 小麦重要病害的功能基因组学和分子生物学研究
小麦产量和品质受到多种病害（如白粉病、条锈病、黄矮病等）的不良影响，挖掘和利用抗性基因是减少病害损失的有效途径。为实现抗性基因的有效挖掘与利用，我们以基因组相对较小和简单的乌拉尔图小麦（Triticum urartu, 2n = 2x = 14，AA）为模式，针对抗病基因的分子和功能互作网络开展研究，以期为植物抗病分子机理增添新知识、为小麦主要病害的有效防治提供有益指导和基因资源。
主要论文（星号标注通讯或共同通讯作者）
Zhang Y, Li D, Zhang D, Zhao X, Cao X, Dong L, Liu J, Chen K, Zhang H, Gao C*, Wang D*. Analysis of the functions of TaGW2 homoeologs in wheat grain weight and protein content traits. Plant Journal 2018; doi:10.1111/tpj.13903.
Wang D*, Zhang K, Dong L, Dong Z, Li Y, Hussain A, Zhai H. Molecular genetic and genomic analysis of wheat milling and end-use traits in China: Progress and perspectives. Crop Journal 2018; 6: 68-81.
Lou H, Dong L, Zhang K, Wang DW, Zhao M, Li Y, Rong C, Qin H, Zhang A, Dong Z, Wang D*. High-throughput mining of E-genome-specific SNPs for characterizing Thinopyrum elongatum introgressions in common wheat. Molecular Ecology Resources 2017; 17: 1318-1329.
Huo N, Dong L, Zhang S, Wang Y, Zhu T, Mohr T, Altenbach S, Liu Z, Dvorak J, Anderson OD, Luo MC, Wang D, Gu YQ*. New insights into structural organization and gene duplication in a 1.75-Mb genomic region harboring the α-gliadin gene family in Aegilops tauschii, the source of wheat D genome. Plant Journal 2017; 92: 571-583.
Wang Z, Li Y, Yang Y, Liu X, Qin H, Dong Z, Zheng S, Zhang K, Wang D*. New insight into the function of wheat glutenin proteins as investigated with two series of genetic mutants. Scientific Reports 2017; 7: 3428.
Zong Y, Wang Y, Li C, Zhang R, Chen K, Ran Y, Qiu JL, Wang D, Gao C*. Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion. Nature Biotechnology 2017; 35: 438-440.
Dong ZY, Yang Y, Zhang KP, Li YW, Wang JJ, Wang ZJ,Liu XJ,Qin HJ,Wang D*. Development of a new set of molecular markers for examining Glu-A1 variants in common wheat and ancestral species. PLoS One 2017; 12: e**.
Wang DW, Li D, Wang J, Zhao Y, Wang Z, Yue G, Liu X, Qin H, Zhang K, Dong L, Wang D*. Genome-wide analysis of complex wheat gliadins, the dominant carriers of celiac disease epitopes. Scientific Reports 2017; 7: 44609.
Zong Y, Xi X, Li S, Chen W, Zhang B, Liu D, Liu B*, Wang D*, Zhang HG*. Allelic variation and transcriptional isoforms of wheat TaMYC1 gene regulating anthocyanin synthesis in pericarp. Frontiers in Plant Science 2017; 8: 1645.
Zhang J, Li B, Yang Y, Mu P, Qian W, Dong L, Zhang K, Liu X, Qin H, Ling H, Wang D*. A novel allele of L-galactono-1,4-lactone dehydrogenase is associated with enhanced drought tolerance through affecting stomatal aperture in common wheat. Scientific Reports 2016; 6: 30177.
Dong L, Huo N, Wang Y, Deal K, Wang D, Hu T, Dvorak J, Anderson OD, Luo MC, Gu YQ*. Rapid evolutionary dynamics in a 2.8-Mb chromosomal region containing multiple prolamin and resistance gene families in Aegilops tauschii. Plant Journal 2016; 87: 495-506.
Zhang J, Zheng H, Li Y, Li H, Liu X, Qin H, Dong L, Wang D*. Coexpression network analysis of the genes regulated by two types of resistance responses to powdery mildew in wheat. Scientific Reports 2016; 6: 23805.
Li Y, An X, Yang R, Guo X, Yue G, Fan R, Li B, Li Z, Zhang K, Dong Z, Zhang L, Wang J, Jia X, Ling HQ, Zhang A*, Zhang X*, Wang D*. Dissecting and enhancing the contributions of high-molecular-weight glutenin subunits to dough functionality and bread quality. Molecular Plant 2015; 8: 332-334.
Dong L, Liu H, Zhang J, Yang S, Kong G, Chu JSC, Chen N*, Wang D*. Single-molecule real-time transcript sequencing facilitates common wheat genome annotation and grain transcriptome research. BMC Genomics 2015; 16: 1039.
Dong Z*, Feng B, Liang H, Rong C, Zhang K, Cao X, Qin H, Liu X, Wang T, Wang D*. Grain-specific reduction in lipoxygenase activity improves flour color quality and seed longevity in common wheat. Molecular Breeding 2015; 35: 150.
Yang Y, Li S, Zhang K, Dong Z, Li Y, An X, Chen J, Chen Q, Jiao Z, Liu X, Qin H, Wang D*. Efficient isolation of ion beam-induced mutants for homoeologous loci in common wheat and comparison of the contributions of Glu-1 loci to gluten functionality. Theoretical and Applied Genetics 2014; 127: 359-372.
He X, Fang J, Li J, Qu B, Ren Y, Ma W, Zhao X, Li B, Wang D, Li Z, Tong Y. A genotypic difference in primary root length is associated with the inhibitory role of transforming growth factor-beta receptor-interacting protein-1 on root meristem size in wheat. Plant Journal 2014; 77: 931-943.
Cao P, Ren Y, Zhang K, Teng W, Zhao X, Dong Z, Liu X, Qin H, Li Z, Wang D*, Tong Y*. Further genetic analysis of a major quantitative trait locus controlling root length and related traits in common wheat. Molecular Breeding 2014; 33: 975-985.
Dong L, Wang F, Liu T, Dong Z, Li A, Jing R, Mao L, Li Y, Liu X, Zhang K*, Wang D*. Natural variation of TaGASR7-A1 affects grain length in common wheat under multiple cultivation conditions. Molecular Breeding 2014; 34: 937-947.
Ling HQ*, Zhao S, Liu D, Wang J, Sun H, Zhang C, Fan H, Li D, Dong L, Tao Y, Gao C, Wu H, Li Y, Cui Y, Guo X, Zheng S, Wang B, Yu K, Liang Q, Yang W, Lou X, Chen J, Feng M, Jian J, Zhang X, Luo G, Jiang Y, Liu J, Wang Z, Sha Y, Zhang B, Wu H, Tang D, Shen Q, Xue P, Zou S, Wang X, Liu X, Wang F, Yang Y, An X, Dong Z, Zhang K, Zhang X, Luo MC, Dvorak J, Tong Y, Wang J, Yang H, Li Z*, Wang D*, Zhang A*, Wang J*. Draft genome of the wheat A-genome progenitor Triticum urartu. Nature 2013; 496: 87-90.
Zhang K, Wang J, Zhang L, Rong C, Zhao F, Peng T, Li H, Cheng D, Liu X, Qin H, Zhang A, Tong Y, Wang D*. Association analysis of genomic loci important for grain weight control in elite common wheat varieties cultivated with variable water and fertiliser supply. PLoS One 2013; 8: e57853.
Dong Z, Yang Y, Li Y, Zhang K, Lou H, An X, Dong L, Gu YQ, Anderson OD, Liu X, Qin H, Wang D*. Haplotype variation of Glu-D1 locus and the origin of Glu-D1d allele conferring superior end-use qualities in common wheat. PLoS One 2013; 8: e74859.
Dong L, Huo N, Wang Y, Deal K, Luo MC, Wang D, Anderson OD, Gu YQ. Exploring the diploid wheat ancestral A genome through sequence comparison at the high-molecular-weight glutenin locus region. Molecular and Genetic Genomics 2012; 287: 855-866.
Feng B, Dong Z, Xu Z, Wang D*, Wang T*. Molecular characterization of a novel type of lipoxygenase (LOX) gene from common wheat (Triticum aestivum L.). Molecular Breeding 2012; 30: 113-124.
Wu C, Feng J, Wang R, Liu H, Yang H, Rodriguez PL, Qin H, Liu X, Wang D*. HRS1 acts as a negative regulator of abscisic acid signaling to promote timely germination of Arabidopsis seeds. PLoS One 2012; 7: e35764.
Wang L, Li Z, Qian W, Guo W, Gao X, Huang L, Wang H, Zhu H, Wu JW, Wang D, Liu D. Arabidopsis purple acid phosphatase AtPAP10 is predominantly associated with the root surface and plays an important role in plant tolerance to phosphate limitation. Plant Physiology 2011; 157: 1283-1299.
Zhang L, Liu D, Guo X, Yang W, Sun J, Wang D, Sourdille P, Zhang A. Investigation of genetic diversity and population structure of common wheat cultivars in northern China using DArT markers. BMC Genomics 2011; 12: 42.
Zhang X, Liu D, Yang W, Liu K, Sun J, Guo X, Li Y, Wang D, Ling H, Zhang A. Development of a new marker system for identifying the complex members of the low-molecular-weight glutenin subunit gene family in bread wheat (Triticum aestivum L.). Theoretical and Applied Genetics 2011; 122: 1503-1516.
Wang GF, Wei X, Fan R, Zhou H, Wang X, Yu C, Dong L, Dong Z, Wang X, Kang Z, Ling H, Shen QH, Wang D*, Zhang X*. Molecular analysis of common wheat genes encoding three types of cytosolic heat shock protein 90 (Hsp90): functional involvement of cytosolic Hsp90s in the control of wheat seedling growth and disease resistance. New Phytologist 2011; 191: 418-431.
Yu C, Li Y, Li B, Liu X, Hao L, Chen J, Qian W, Li S, Wang G, Bai S, Ye H, Qin H, Shen Q, Chen L, Zhang A, Wang D*. Molecular analysis of phosphomannomutase (PMM) genes reveals a unique PMM duplication event in diverse Triticeae species and the main PMM isozymes in bread wheat tissues. BMC Plant Biology 2010; 10: 214.
Dong L, Zhang X, Liu D, Fan H, Sun J, Zhang Z, Qin H, Li B, Hao S, Li Z, Wang D*, Zhang A*, Ling HQ*. New insights into the organization, recombination, expression and functional mechanism of low molecular weight glutenin subunit genes in bread wheat. PLoS one 2010; 5: e13548.
Feng B, Dong Z, Xu Z, An X, Qin H, Wu N, Wang D*, Wang T*. Molecular analysis of lipoxygenase (LOX) genes in common wheat and phylogenetic investigation of LOX proteins from model and crop plants. Journal of Cereal Science 2010; 52: 387-394.
Tran HT, Qian W, Hurley BA, She YM, Wang D, Plaxton WC. Biochemical and molecular characterization of AtPAP12 and AtPAP26: the predominant purple acid phosphatase isozymes secreted by phosphate-starved Arabidopsis thaliana. Plant, Cell & Environment 2010; 33: 1789-1803.
He Y, Jones HD, Chen S, Chen XM, Wang D, Li KX, Wang DS, Xia LQ. Agrobacterium-mediated transformation of durum wheat (Triticum turgidum L. var. durum cv Stewart) with improved efficiency. Journal of Experimental Botany 2010; 61: 1567-1581.
Jia J, Liu C, Wang D, Chao S, Wang M, Qi X. Mike Gale and cereal genetics research in China. Molecular Breeding 2010; 25: 365-367.
Zhang LY, Liu DC, Guo XL, Yang WL, Sun JZ, Wang D, Zhang A. Genomic distribution of quantitative trait loci for yield and yield-related traits in common wheat. Journal of Integrative Plant Biology 2010; 52: 996-1007.
An XL, Yu CM, Wang D*. Assessment of plant gene functions using viral vectors. In: Wang A, ed. Principles and Practice of Advanced Technology in Plant Virology. Research Signpost 2010; pp 311-330.
Yang H, Liu H, Li G, Feng J, Qin H, Liu X, Xue H, Wang D*. Reduction of root flavonoid level and its potential involvement in lateral root emergence in Arabidopsis thaliana grown under low phosphate supply. Functional Plant Biology 2009; 36: 1-10.
Bu Q, Li H, Zhao Q, Jiang H, Zhai Q, Zhang J, Wu X, Sun J, Xie Q, Wang, D, Li C. The Arabidopsis RING finger E3 ligase RHA2a is a novel positive regulator of abscisic acid signaling during seed germination and early seedling development. Plant Physiology 2009; 150: 463-481.
Ding Z, Li S, An X, Liu X, Qin H, Wang D*. Transgenic expression of MYB15 confers enhanced sensitivity to abscisic acid and improved drought tolerance in Arabidopsis thaliana. Journal of Genetics and Genomics 2009; 36: 17-29.
Liu H, Yang H, Wu C, Feng J, Liu X, Qin H, Wang D*. Overexpressing HRS1 confers hypersensitivity to low phosphate-elicited inhibition of primary root growth in Arabidopsis thaliana. Journal of Integrative Plant Biology 2009; 51: 382-392.
Qin C, Qian W, Wang W, Wu Y, Yu C, Jiang X, Wang D*, Wu P*. GDP-mannose pyrophosphorylase is a genetic determinant of ammonium sensitivity in Arabidopsis thaliana. Proceedings of the National Academy of Sciences of USA 2008; 105: 18308-18313.
Xia Z, Wang Y, Du Z, Li J, Zhao R, Wang D*. A potential nuclear envelope-targeting domain and an arginine-rich RNA binding element identified in the putative movement protein of the GAV strain of Barley yellow dwarf virus. Functional Plant Biology 2008; 35: 40-50.
Li Z, Zhang X*, Zhang H, Cao S, Wang D*, Hao S, Li L, Li H, Wang X. Isolation and characterization of a novel variant of HMW glutenin subunit gene from the St genome of Pseudoroegneria stipifolia. Journal of Cereal Science 2008; 47: 429-437.
Wang J, Sun J, Liu D, Yang W, Wang D, Tong Y, Zhang A. Analysis of Pina and Pinb alleles in the micro-core collections of Chinese wheat germplasm by ecotilling and identification of a novel Pinb allele. Journal of Cereal Science 2008; 48: 836-842.
Yuan Y, Wu H, Wang N, Li J, Zhao W, Du J, Wang D, Ling H. FIT interacts with AtBHLH38 and AtBHLH39 in regulating iron uptake gene expression of iron homeostasis in Arabidopsis. Cell Research 2008; 18: 385-397.
Qian W, Yu C, Qian H, Liu X, Zhang A, Johansen IE, Wang D*. Molecular and functional analysis of phosphomannomutase (PMM) from higher plants and genetic evidence for the involvement of PMM in ascorbic acid biosynthesis in Arabidopsis and Nicotiana benthamiana. Plant Journal 2007; 49: 399-413.
Zhou H, Li S, Deng Z, Wang X, Chen T, Zhang J, Chen S, Ling H, Zhang A, Wang D*, Zhang X*. Molecular analysis of three new receptor-like kinase genes from hexaploid wheat and evidence for their participation in wheat hypersensitive response to stripe rust fungus infection. Plant Journal 2007; 52: 420-434.
Liu X, Qian W, Liu X, Qin H, Wang D*. Molecular and functional analysis of hypoxanthine-guanine phosphoribosyltransferase from Arabidopsis thaliana. New Phytologist 2007; 175: 448-461.
Mo P, Zhu Y, Liu X, Zhang A, Yang C, Wang D*. Identification of two phosphatidylinositol/ phosphatidylcholine transfer protein genes that are predominately transcribed in the flowers of Arabidopsis thaliana. Journal of Plant Physiology 2007; 164: 478-486.
Niu Y, Chen K, Wang J, Liu X, Qin H, Zhang A, Wang D*. Molecular and functional characterization of sphingosine-1-phosphate lyase homolog from higher plants. Journal of Integrative Plant Biology 2007; 49: 323-335.
Cao S, Xu H, Li Z, Wang X, Wang D, Zhang A, Jia X, Zhang X. Identification and characterization of a novel Ag. intermedium HMW-GS gene from T. aestivum-Ag. intermedium addition lines TAI-I series. Journal of Cereal Science 2007; 45: 293-301.
Chen K, Xu M, Li G, Liang H, Xia Z, Liu X, Zhang J, Zhang A, Wang D*. Identification of AtENT3 as the main transporter for uridine uptake in Arabidopsis roots. Cell Research 2006; 16: 377-388.
Liu X, Liu X, Zhang J, Xia Z, Liu X, Qin H, Wang D*. Molecular and functional characterization of sulfiredoxin homologs from higher plants. Cell Research 2006; 16: 287-296.
Zhang J, Zhu H, Liang H, Liu K, Zhang A, Ling HQ, Wang D*. Further analysis of the function of AtBHLH29 in regulating the iron uptake process in Arabidopsis thaliana. Journal of Integrative Plant Biology 2006; 48: 75-84.
Sun X, Wang D*, Hu S, Liu X, Zhang A, Qian W, Hao S. Characterization of the HMW glutenin subunits from Aegilops searsii and identification of a novel variant HMW glutenin subunit. Theoretical and Applied Genetics 2006; 113: 631-641.
Kan Y, Wan Y, Beaudoin F, Leader DJ, Edwards K, Poole R, Wang D, Mitchell RAC, Shewry PR. Transcriptome analysis reveals differentially expressed storage protein transcripts in seeds of Aegilops and wheat. Journal of Cereal Sciences 2006; 44: 75-85.
Yuan YX, Zhang J, Wang D, Ling HQ. AtbHLH29 of Arabidopsis thaliana is a functional ortholog of tomato FER involved in controlling iron acquisition in strategy I plants. Cell Research 2005; 15: 613-21.
Zhu H, Qian W, Lu X, Li D, Liu X, Liu K, Wang D*. Expression patterns of purple acid phosphatase genes in Arabidopsis organs and functional analysis of AtPAP23 predominantly transcribed in flower. Plant Molecular Biology 2005; 59: 581-594.
Wan Y, Yan Z, Liu K, Zheng Y, D’Ovidio R, Shewry PR, Halford NG, Wang D. Comparative analysis of the D genome-encoded high-molecular weight subunits of glutenin. Theoretical and Applied Genetics 2005; 111: 1183-1190.
Liu K, Xia Z, Zhang Y, Wen Y, Wang D, Brandenburg K, Harris F, Phoenix DA. Interaction between the movement protein of barley yellow dwarf virus and the cell nuclear envelope: role of a putative amphiphilic alpha-helix at the N-terminus of the movement protein. Biopolymers 2005; 79: 86-96.
Aparicio F, Thomas CL, Lederer C, Niu Y, Wang D, Maule AJ. Virus induction of heat shock protein 70 reflects a general response to protein accumulation in the plant cytosol. Plant Physiology 2005; 138: 529-536.
Cui X, Li G, Wang D, Hu D, Zhou X. A Begomovirus DNA β-encoded protein binds DNA, functions as a suppressor of RNA silencing, and targets the cell nucleus. Journal of Virology 2005; 79: 10764-10775.
Li W, Wan Y, Liu Z, Liu K, Liu X, Li B, Li Z, Zhang X, Dong Y, Wang D*. Molecular characterization of HMW glutenin subunit allele 1Bx14: further insights in to the evolution of Glu-B1-1 alleles in wheat and related species. Theoretical and Applied Genetics 2004; 109: 1093-1104.
Bai J, Liu K, Jia X, Wang D*. An analysis of homoeologous microsatellites from Triticum urartu and Triticum monococcum. Plant Science 2004; 166: 341-347.
Li G, Liu K, Baldwin S, Wang D*. Equilibrative nucleoside transporters of Arabidopsis thaliana: cDNA cloning, expression pattern and analysis of transport activities. Journal of Biological Chemistry 2003; 278: 35732-35742.
Xie C, Zhang JS, Zhou HL, Li J, Zhang ZG, Wang D, Chen SY. Serine/threonine kinase activity in the putative histidine kinase-like ethylene receptor NTHK1 from tobacco. Plant Journal 2003; 33: 385-393.
Liu Z, Yan Z, Wan Y, Liu K, Zheng Y, Wang D*. Analysis of HMW glutenin subunits and their coding sequences in two diploid Aegilops species. Theoretical and Applied Genetics 2003; 106: 1368-1378.
Roberts IM, Wang D, Thomas CL, Maule AJ. Pea seed-borne mosaic virus seed transmission exploits novel symplastic pathways to infect the pea embryo and is, in part, dependent upon chance. Protoplasma 2003; 222: 31-43.
Li D, Zhu H, Liu K, Leggewie G, Udvardi M, Wang D*. Purple acid phosphatases of Arabidopsis thaliana: comparative analysis and differential regulation by phosphate deprivation. Journal of Biological Chemistry 2002; 277: 27772-27781.
Wan Y, Wang D, Shewry R, Halford G. Isolation and characterization of five novel high molecular weight subunit of glutenin genes from Triticum timopheevi and Aegilops cylindrica. Theoretical and Applied Genetics 2002; 104: 828-839.
Li J, Wang D*. Cloning and in vitro expression of the cDNA encoding a putative nucleoside transporter from Arabidopsis thaliana. Plant Science 2000; 157: 23-32.
Wan Y, Liu KF, Wang D*, Shewry PR. High molecular weight subunits in the Cylindropyrum and Vertebrata section of the Aegilops genus and identification of subunits related to those encoded by the Dx alleles of common wheat. Theoretical and Applied Genetics 2000; 101: 879-884.
Wang D, Macfarlane SA, Maule AJ*. Viral determinants of pea early browning virus seed transmission in pea. Virology 1997; 234: 112-117.
Wang D, Maule AJ*. Contrasting patterns in the spread of two seed‐borne viruses in pea embryos. Plant Journal 1997; 11: 1333-1340.
Aranda MA, Escaler M, Wang D. Maule AJ*. Induction of HSP70 and polyubiquitin expression associated with plant virus?replication. Proceedings of the National Academy of Sciences of USA 1996; 93: 15289-15293.
Wang D, Maule AJ*. Inhibition of host gene expression associated with plant virus replication. Science 1995; 267: 229-231.
Wang D, Maule AJ*. A model for seed transmission of a plant virus: genetic and structural analyses of pea embryo invasion by pea seed-borne mosaic virus. Plant Cell 1994; 6: 777-787.