中文关键词:金纳米棒荧光探针Hg²⁺ 英文关键词:Gold nanorodsFluorescent probeHg²⁺ 基金项目:国家自然科学基金项目（21804141）资助

作者	单位	E-mail
李瑞军	中国药科大学理学院 分析化学教研室 南京 211198
朱长龙	中国药科大学理学院 分析化学教研室 南京 211198
季一兵	中国药科大学理学院 分析化学教研室 南京 211198	jiyibing@msn.com

摘要点击次数:365 全文下载次数:0 中文摘要: 建立了一种以牛血清白蛋白功能化的金纳米棒（BSA-Cys-GNRs）为荧光探针检测Hg²⁺的新方法。以半胱氨酸作为连接臂成功将牛血清白蛋白修饰在金纳米棒表面，通过紫外可见吸收光谱、荧光光谱、红外光谱和荧光倒置显微镜等多种分析方法对材料进行表征。研究发现，在295nm波长光激发下，BSA-Cys-GNRs探针在338nm显示强荧光，而Hg²⁺能够有效地猝灭BSA-Cys-GNRs的荧光。对一系列影响猝灭效果的因素进行考察，得出pH 4.0、孵育时间5.0min为最佳检测条件。Mn²⁺、K⁺、Ni²⁺、Na⁺、Cr³⁺、Cd²⁺、Mg²⁺、Cu²⁺、Ca²⁺、Al³⁺和Zn²⁺对BSA-Cys-GNRs的荧光信号没有明显的影响。当Hg²⁺的浓度为0.04444~8.888μmol·L^-1时，荧光猝灭效率与Hg²⁺的浓度之间存在良好的线性关系，检测限为8.08nmol·L^-1。将该方法用于环境水样中Hg²⁺的检测，回收率为98.9%~105.0%。 英文摘要: Bovine serum albumin (BSA) functionalized gold nanorods (BSA-Cys-GNRs) as a fluorescence probe for the detection of Hg²⁺ has been developed in this work. BSA was successfully modified on the surface of gold nanorods with cysteine as the linker. The materials were characterized by UV-Vis, FL, FT-IR and inverted fluorescence microscopy. The BSA-Cys-GNRs displays strong fluorescence emission peak at 338 nm under excitation at 295 nm. The fluorescence of BSA-Cys-GNRs is strongly quenched upon addition of Hg²⁺ ions. Effective detection conditions are pH 4.0 and incubating time 5.0 min. Mn²⁺, K⁺, Ni²⁺, Na⁺, Cr³⁺, Cd²⁺, Mg²⁺, Cu²⁺, Ca²⁺, Al³⁺ and Zn²⁺ are examined under identical conditions, and all of them do not induce any noticeable responses to BSA-Cys-GNRs. There is a good linear dependence of fluorescence intensity on Hg²⁺ concentration in the range of 0.04444~8.888 μmol·L^-1 with the detection limit of 8.08 nmol·L^-1. Furthermore, the method has been applied for the determination of Hg²⁺ in water samples, and the recoveries are in the range of 98.9%~105.0%. 查看全文查看/发表评论下载PDF阅读器 相关附件：版权转让声明书审稿意见回复文件附件1 -->