李智立
课题组负责人：李智立，男，教授，理学博士，博士生导师
研究方向：慢性疾病早期预警及其预后评估新技术新方法
主要研究内容：
血清/血浆成分分析方法及其应用：
? 肿瘤个体化诊断及预后标志物筛选；
?生物大分子复合物分析方法研究及其应用；
?糖蛋白糖链结构分析方法研究及其应用;
?代谢物分析方法研究及其应用.
质谱成像及其应用：
?原位研究代谢物、蛋白质变化与疾病发生之间的关系；
?原位研究单细胞代谢物、蛋白质变化与细胞表型之间的关系。
主要技术手段：质谱技术(9.4T FTICR MS和LTQ等）、新型色谱分离技术、凝胶分离技术等。
科研合作及技术服务：为了充分利用本课题组已建立的技术平台和方法，课题组在蛋白质鉴定、完整蛋白质分子量测定、二硫键分析、N-和C-端测序、糖基化修饰和代谢物鉴定等方面提供技术服务或合作研究。
招募自愿者：经过多年对血液成分变化与慢性疾病发生或肿瘤预后之间关系的研究，本课题组发现一组与慢性疾病发生和恶性肿瘤复发相关的新型个体化标志物 (国际专利申请号: PCT/CN2012/071113和国家专利号：ZL2.1)。经中国医学科学院基础医学研究所伦理委员会批准(项目编号：004-2013)，本课题组长期开展大人群的“血液成分变化与健康状态关系研究”。
入选标准：年龄20-70岁，健康人群或患有单一慢性疾病患者或肿瘤患者。
参加者：健康人群每年提供一次血液(3-4毫升)；恶性肿瘤患者或其它慢性疾病患者将依据病情适当增加检测次数。我们将提供给参加者血液中上述标志物含量的信息。详细内容请利用下面的联系方式了解。

本课题组提供博士后和课题组项目聘用人员岗位，有意者请电话或邮件联系。
联系方式：
中国医学科学院基础医学研究所/北京协和医学院 基础学院
生物物理学及结构生物学系
北京市东城区东单三条五号，100005
电话：
E-mail：lizhili@ibms.pumc.edu.cn; zhililily@aliyun.com


近5年发表的研究论文：
1. Mo Zhang, Qing Wang, YupinXu, Lei Guo, Zhizhen Lai and Zhili Li*, Graphitic carbon nitridequantum dots as analytical probe for viewing sialic acid on the surface ofcells and tissues, Analytica Chimica Acta, 2020, 1095,204-211.
2. Dan Zhang, Xiaoou Li,Xiaofeng Liu, Yanmin Wang, Mo Zhang, Qin Wang, Tianjing Chen, Zhili Li*, Disease-specific IgG Fcglycosylation ratios as personalized biomarkers to differentiate non-small celllung Cancer from benign lung diseases, Proteomics Clinical Applications,2020,DOI:10.1002/prca.
3. Na Li, Yuming Qiu, YunpengWu, Mo Zhang, Zhizhen Lai, Qing Wang, Yuying Du, Lei Guo, Shuai Liu, Zhili Li*, Association of serum totalfatty acids with type 2 diabetes, Clinica Chimica Acta, 2020, 500,59-68.
4. Yunpeng Wu, RuiZhou,Zhizhen Lai1, Wei Wang, Na Li, Yuying Du, Lei Guo, Yuming Qiu, Qing-Tao Wang*,and Zhili Li*，Monitoring novel modifiedhemoglobin using mass spectrometry contributes to accurate blood glucosemanagement of the Han Chinese population, Clinica Chimica Acta, 2019, 489,124-129.
5. Tianjing Chen, Chengyan He,Mo Zhang, Xiaoou Li, Xiaofeng Liu, Yujie Liu, Dan Zhang, and Zhili Li*, Disease-specific haptoglobin-βchain N-glycosylation asbiomarker to differentiate non-small cell lung cancer from benign lungdiseases, Journal of Cancer, 2019, 10, 5628-5637.
6. Y. H. Sun, J. Li, H. J. Shu,Z. L. Li, J. M. Qian, Serum immunoinflammation?related protein complexesdiscriminate between inflammatory bowel disease and colorectal cancer, Clinicaland Translational Oncology, 2019, 21, 1680-1686.
7. Shuai Guo, Yanmin Wang, DanZhou, Yupin Xu, Tianjing Chen, Zhili Li*, Association of alterationof nucleosides and nucleotides with gastric cancer microenvironment, InternationalJournal of Mass Spectrometry, 2018, 434, 37–42.
8. Yupin Xu, Mo Zhang, QingWang, Zhili Li*, In situ detecting changes in membranelipid phenotypes of macrophages cultured in different cancer microenvironmentsusing mass spectrometry, Analytica Chimica Acta, 2018, 1026,101-108.
9. YujieLiu, Yunpeng Wu, Yanmin Wang, Mo Zhang, and Zhili Li*，Increased Levels of Serum Protein ComplexesAre Associated with Type 2 Diabetes，International Journal of Medical Sciences, 2018, 15(3), 210-216.
10.Mo Zhang, Yujie Liu, Dan Zhang,Tianjing Chen, Zhili Li*, Facileand Selective Enrichment of Intact Sialoglycopeptides Using Graphitic CarbonNitride, Analytical Chemistry, 2017, 89(15) , 8064-8069.
11. Shuai Guo, Dan Zhou, Mo Zhang, TiejunLi, Yujie Liu, Yupin Xu, Tianjing Chen, ZhiliLi*, Monitoring changes of docosahexaenoic acid-containing lipids duringthe recovery process of traumatic brain injury in rat using mass spectrometryimaging, Scientific Reports, 2017, 7. e5054.
12.Yumei Guo, Junling Ren, Xiaoou Li,Xiaofeng Liu, Ning Liu, Yanmin Wang, ZhiliLi*, Simultaneous Quantification of Serum Multi-Phospholipids as PotentialBiomarkers for Differentiating Different Pathophysiological states of lung,stomach, intestine, and pancreas, Journal of Cancer, 2017, 8(12),2191-2204.
13.Dan Zhou, Shuai Guo, Mo Zhang, YujieLiu, Tianjing Chen, Zhili Li*,Mass spectrometry imaging of small molecules in biological tissues usinggraphene oxide as a matrix, Analytica Chimica Acta, 2017, 962,52-59.
14.Xiaodong Wang, Yujie Liu, Fenjie Li, Zhili Li*, Poplarcatkin: a natural biomaterial for highly specific and efficient enrichment ofsialoglycopeptides, ChineseChemical Letter, 2017, 28,1018-1026.
15.Chenchen Xu, Dan Zhou, Yixin Luo, ShuaiGuo, Tao Wang, Jie Liu*, Yuehua Liu*, ZhiliLi*, Tissue and serum lipidome shows altered lipidcomposition with diagnostic potential in mycosis fungoides, Oncotarget, 2017, 8,48041-48050.
16. JunlingRen, Dan Zhang, Yujie Liu, Ruiqing Zhang, Huiling Fang, Shuai Guo, Dan Zhou, MoZhang, Yupin Xu, Ling Qiu*, Zhili Li*,Simultaneous Quantification of Serum Nonesterified and Esterified Fatty Acidsas Potential Biomarkers to Differentiate Benign Lung Diseases from Lung Cancer, ScientificReports, 2016, 6, e34201.
17. DanZhang, Bingchao Chen, Yanmin Wang, Peng Xia, Chengyan He, Yujie Liu, RuiqingZhang, Mo Zhang, Zhili Li* Disease-specificIgG Fc N-glycosylation as personalized biomarkers to differentiate gastriccancer from benign gastric diseases, Scientific Reports, 2016,6, e25957, DOI: 10.1038/srep25957.
18.ManwenHe, Shuai Guo, Junling Ren, Zhili Li*,In Situ Characterizing Membrane Lipid Phenotype of Human Lung Cancer Cell LinesUsing Mass Spectrometry Profiling, Journalof Cancer, 2016, 7(7), 810-816.
19. YapingZhang, Chengyan He, Ling Qiu, Yanmin Wang, Xuzhen Qin, Yujie Liu, Zhili Li*, Serum unsaturated free fattyacids: a potential biomarker panel for early-stage detection of colorectalcancer, Journal of Cancer, 2016, 7(4), 477-483.
20. YujieLiu, Yuanjun Liu, Dan Zhang, Ruiqing Zhang, Zhili Li*, Kapokfiber: a natural biomaterial for highly specific and efficient enrichment ofsialoglycopeptides, Analytical Chemistry,2016, 88(2),1067-1072.
21. Yujie Liu, Dan Zhang, Yuhang Cheng*, Zhili Li*, Elevated serumimmunoinflammation-related protein complexes are associated with psychosis, PsychiatryResearch, 2015, 230, 96-101.
22. YapingZhang, Ling Qiu, Chengyan He, Yanmin Wang, Yujie liu, Dan Zhang, Zhili Li^*, Serum unsaturatedfree fatty acids: a potential biomarker panel for differentiating benignthyroid diseases from thyroid cancer, Journal of Cancer, 2015,6(12), 1276-1281.
23.ShuaiGuo, Yanmin Wang, Dan Zhou, and Zhili Li*, Electric Field-Assisted Matrix Coating MethodEnhances the Detection of Small Molecule Metabolites for Mass SpectrometryImaging, AnalyticalChemistry, 2015, 87(12), 5860-5865.
24.ManwenHe, Shuai Guo, Zhili Li*, In situcharacterizing membrane lipid phenotype of breast cancer cells using massspectrometry profiling, Scientific Reports, 2015, 5, 11298.
25.ManwenHe, Shuai Guo, Zhili Li*, Dynamicallyin situ monitoring lipid changes in DHA-treated breast cells by matrix-assistedlaser desorption/ionization Fourier transform ion cyclotron resonance massspectrometry, Rapid Commun Mass Spectrom, 2015, 29(10), 987-990.
26. Gaoguang Song, Yujie Liu, Yanying Wang,Guanjun Ren, Shuai Guo, Junling Ren, Li Zhang*, ZhiliLi*, Personalized biomarkers to monitor disease progression in advanced non-small-cell lung cancerpatients treated with icotinib,Clinica Chimica Acta, 2015, 440, 44-48.
27. YapingZhang, Ling Qiu,Yanmin Wang, Chengyan He, Xuzhen Qin, Yujie liu, Zhili Li*, Unsaturated free fatty acids: a potentialbiomarker panel for early detection of gastric cancer, Biomarkers, 2014, 19(8),667-673.
28.ShuaiGuo, Yanmin Wang, Dan Zhou, Zhili Li*, Significantly increased monounsaturated lipidsrelative to polyunsaturated lipids in six types of cancer microenvironment areobserved by mass spectrometry imaging, ScientificReports, 2014, 4, 5959.
29. YapingZhang, Chengyan He, Ling Qiu, Yanmin Wang, Li Zhang, Xuzhen Qin, Yujie Liu, DanZhang, Zhili Li*, Serum Unsaturated Free Fatty Acids: Potential Biomarkersfor Early Detection and Disease Progression Monitoring of Non-SmallCell Lung Cancer, Journal of Cancer,2014, 5(8), 706-714.
30. YapingZhang, Lina Song, Ning Liu, Chengyan He*, Zhili Li*, Decreasedserum levels of free fatty acids are associated with breast cancer, ClinicaChimica Acta, 2014, 437,31-37.
31. Shuai Guo, Ling Qiu, Yanming Wang, Xuzhen Qin, Hui Liu,Manwen He, Yaping Zhang, Zhili Li*and Xiaohong Chen*, Tissue imaging and serumlipidomic profiling for screening potential biomarkers of thyroid tumors bymatrix-assisted laser desorption/ionization-Fourier transform ion cyclotronresonance mass spectrometry, Analytical and Bioanalytical Chemistry, 2014, 406, 4357-4370.
32.Yaping Zhang, Ling Qiu, Yanmin Wang, Xuzhen Qin, Zhili Li*, High-throughput andhigh-sensitivity quantitative analysis of serum unsaturated fatty acids by chip-based nanoelectrospray ionization-Fourier transformion cyclotron resonance mass spectrometry: early stage diagnostic biomarkers of pancreatic cancer. Analyst, 2014,139 (7), 1697-1706.
33.Yanying Wang, Gaoguang Song, Yanmin Wang, Ling Qiu, XuzhenQin, Hui Liu, Fang Li, Xiaodong Wang, Fenjie Li, Shuai Guo, Yaping Zhang, Zhili Li*, Elevated serum levels ofcirculating immunoinflammation-related protein complexes are associated withcancer. Journal of Proteome Research, 2014, 13 (2), 710–719.
34. Guoqiang Chen, Hexiang Li, Ling Qiu, Xuzhen Qin, Hui Liu, Zhili Li^*, Change in fucosylated IgG₂ Fc-glycoforms in pancreatitis and pancreatic adenocarcinoma: A promising diseaseclassification model. Analytical and Bioanalytical Chemistry, 2014, 406, 267-273.
35. Yaping Zhang, Yanmin Wang, Shuai Guo, Yumei Guo, Hui Liu, Zhili Li*, Ammonia-treatedN-(1-naphthyl) ethylenediamine dihydrochloride as a novel matrix for rapidquantitative and qualitative determination of serum free fatty acids bymatrix-assisted laser desorption/ionization-Fourier transform ion cyclotronresonance mass spectrometry, Analytica Chimica Acta, 2013,974, 82-89.
36. Fenjie Li,Xiaodong Wang, Yujie Liu, Hui Liu, ZhiliLi* Dephosphorylation of Intact Glycoprotein toGreatly Improve Digestion Efficiency Coupledwith Matrix-Assisted Laser Desorption/Ionization- Fourier Transform IonCyclotron Resonance Mass Spectrometric Analysis. Analytica Chimica Acta, 2013,787, 140-147. .
37.Guoqiang Chen, YanminWang, Xuzhen Qin, Hexiang Li, Yumei Guo, Yanying Wang, Hui Liu, Xiaodong Wang,Gaoguang Song, Fang Li, Fenjie Li, Shuai Guo, Ling Qiu, Zhili Li* Change in IgG1 Fc N-linked glycosylation inhuman lung cancer: Age andsex-related diagnostic potential. Electrophoresis 2013, 34(16),2407-2416.DOI:10.1002/elps..
38.Guoqiang Chen, Li Zhang,Yanying Wang, Yumei Guo, Hui Liu, ZhiliLi*. Probing gender-specific change in IgG1 Fc-galactosylation in non-small cell lungcancer patients treated with epidermal growth factor receptor inhibitor,icotinib hydrochloride, using matrix-assisted laser desorption/ionizationFourier transform ion cyclotron resonance mass spectrometry, Rapid Commun Mass Spectrom 2013, 27(10), 1168-1172.
39.FangLi, Xuzhen Qin, Haiquan Chen, Ling Qiu, Yumei Guo, Hui Liu, Guoqiang ChenGaoguang Song, Xiaodong Wang, Fenjie Li, Shuai Guo, Baohua Wang and Zhili Li*, Lipid profiling for earlydiagnosis and progression of colorectal cancer using direct-infusionelectrospray ionization Fourier transform ion cyclotron resonance massspectrometry, Rapid Communications in Mass Spectrometry, 2013, 27(1), 24-34.
40.YumeiGuo, Xianmin Wang, Ling Qiu, Xuzhen Qin, Hui Liu, Yanying Wang, Fang Li,Xiaodong Wang, Guoqiang Chen, Gaoguang Song, Fenjie Li, Shuai Guo, Zhili Li*,Probing gender-specific lipid metabolites and diagnostic biomarkers for lungcancer using Fourier transform ion cyclotron resonance mass spectrometry, Clinica Chimica Acta,2012, 414, 135-141.
41.XiaodongWang, Fenjie Li, Gaoguang Song, Shuai Guo, Hui Liu, Guoqiang Chen, Zhili Li*, Broad-spectrum four-dimensionalorthogonal electrophoresis: anovel comprehensivelyfeasible system for protein complexomics investigation, Molecular & Cellular proteomics, 2012,11(9), 786-799.
42.GuoqiangChen, Yanmin Wang, Ling Qiu, Xuzhen Qin, Hui Liu, Xiaodong Wang, Yanying Wang,Gaoguang Song, Fang Li, Yumei Guo, Fenjie Li, Shuai Guo, Zhili Li*, HumanIgG Fc-glycosylation profiling reveals association with age, sex, female sexhormones and thyroid cancers, Journalof Proteomics, 2012, 75(10), 2824-2834.

授权或已申请专利：
1. 一组监测癌症疾病进展和或耐药性产生的免疫球蛋白G糖基化修饰标志物，申请号：1.3
2. 确定或辅助监测癌症进展的个体化标志物，专利号：1.3.
3. 一种富集分离唾液酸化糖肽的新材料及其应用, 申请号：9.8
4. 基于脂质代谢谱的个体化肿瘤进展监测标志物及其应用, 申请号：4.1
5. 一种富集唾液酸化糖肽的材料及其应用，申请号：6.6
6. 一种原位质谱分析的常压离子源装置，专利号：ZL8.2
7. 一种用于质谱成像的基质喷涂装置，专利号：ZL7.x
8. 一种富集唾液酸化糖肽、唾液酸化聚糖或唾液酸化糖苷的方法, 专利号：0.9 。
9. 蛋白质复合物及其谱以及其在疾病诊断、进展评估和药效评估中的应用，申请号：PCT/CN2012/071113
10.蛋白质复合物及其在肿瘤诊断和/或预后评估中的应用，专利号：ZL2.1。
11. 一种多发性骨髓瘤特异性蛋白及其专用检测试剂盒，专利号：ZL4.7。
12. 一种糖肽富集分离材料及其应用，申请号：ZL89.x。
13. 一种蛋白质复合物或复合体的三维凝胶电泳分离方法，申请号：6.x。