菜薹BrWRKY57的特性及其对BrPPH1和BrNCED3的调控

曾泽湘¹, 肖显梅¹, 谭小丽¹, 范中奇^2,*(), 陈建业^1,*()

¹华南农业大学园艺学院,南方园艺产品保鲜教育部工程研究中心/广东省果蔬保鲜重点试验室,广州 510642
²福建农林大学食品科学学院,亚热带特色农产品采后生物学福建省高校重点试验室,福州 350002

收稿日期:2020-07-17出版日期:2021-03-25发布日期:2021-04-02
通讯作者:范中奇,陈建业E-mail:ffanzqi@163.com;chenjianye@scau.edu.cn

基金资助:国家自然科学基金面上项目(31671897)

Characteristics of the Transcription Factor BrWRKY57 and Its Regulation on BrPPH1 and BrNCED3

ZENG Zexiang¹, XIAO Xianmei¹, TAN Xiaoli¹, FAN Zhongqi^2,*(), CHEN Jianye^1,*()

¹Engineering Research Center of Southern Horticultural Products Preservation,Ministry of Education/Guangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables,College of Horticulture,South China Agricultural University,Guangzhou 510642,China
²Key Laboratory of Postharvest Biology for Subtropical Special Agricultural Products of Fujian Province,College of Food Science,Fujian Agriculture and Forestry University,Fuzhou 350002,China

Received:2020-07-17Online:2021-03-25Published:2021-04-02
Contact:FAN Zhongqi,CHEN Jianye E-mail:ffanzqi@163.com;chenjianye@scau.edu.cn

摘要/Abstract

摘要： 从菜薹叶片中分离获得1个WRKY转录因子,命名为BrWRKY57。氨基酸序列比对及进化树分析发现BrWRKY57与拟南芥AtWRKY57同源性较高,含有1个WRKY保守结构域,且同属于WRKY转录因子家族Ⅱc亚族。实时荧光定量PCR分析表明,BrWRKY57随着菜薹叶片衰老表达水平升高,外源脱落酸（abscisic acid,ABA）处理显著诱导其表达。亚细胞定位和转录活性分析表明,BrWRKY57定位于细胞核,且在酵母和烟草体内都具有转录激活活性。双荧光素酶瞬时表达试验显示,BrWRKY57可以激活叶绿素降解相关基因BrPPH1和ABA合成相关基因BrNCED3的启动子活性。以上研究结果表明,BrWRKY57参与菜薹叶片衰老过程可能与影响叶绿素降解和ABA合成相关基因的表达有关。
中图分类号:
S634.5

引用本文

曾泽湘, 肖显梅, 谭小丽, 范中奇, 陈建业. 菜薹BrWRKY57的特性及其对BrPPH1和BrNCED3的调控[J]. 园艺学报, 2021, 48(3): 518-530.
ZENG Zexiang, XIAO Xianmei, TAN Xiaoli, FAN Zhongqi, CHEN Jianye. Characteristics of the Transcription Factor BrWRKY57 and Its Regulation on BrPPH1 and BrNCED3[J]. Acta Horticulturae Sinica, 2021, 48(3): 518-530.





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