Effects of nitrogen fertilizer in whole growth duration applied in the middle and late tillering stage on yield and quality of dry direct seeding rice under “solo-stalk” cultivation mode
ZHAO Jie,, LI Shao-Ping, CHENG Shuang, TIAN Jin-Yu, XING Zhi-Peng,*, TAO Yu, ZHOU Lei, LIU Qiu-Yuan, HU Ya-Jie, GUO Bao-Wei, GAO Hui, WEI Hai-Yan, ZHANG Hong-Cheng,*Jiangsu Provincial Key Laboratory of Crop Cultivation and Physiology, Yangzhou University/Jiangsu Collaborative Innovation Center of Modern Industrial Technology for Grain Crops/Rice Industry Engineering Technology Research Institute, Yangzhou 225009, Jiangsu, China
The Agricultural Science and Technology Innovation and Extension Project of Jiangsu Province, the China Agriculture Research System.CARS-01-27 The earmarked fund for Jiangsu Agricultural Industry Technology System.JATS(2019)444 The Agricultural Technology Independent Innovation Fund of Jiangsu Province.CX(20)1012 The Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
Abstract In a rice-wheat cropping system, dry direct seeding rice growth was directly affected by harvest dates of the previous crop of wheat, the return of full wheat straw to the field, and the poor quality of tillage and land preparation. A “solo-stalk” cultivation mode with main stem panicles by late sowing dates, large sowing rates and high basic seedlings was commonly used in dry direct seeding. However, the nitrogen fertilizer management of high-quality and high-yield dry direct-seeding rice for the “solo-stalk” cultivation mode was still lacking in systematic research. With high-quality japonica rice Nanjing 9108, 380×104 hm-2 basic seedlings were realized by mechanical dry direct seeding method. The leaf age treatments of 6, 7, 8, 9, and 10 leaf age and nitrogen application amount treatments of 180 and 225 kg hm-2 were designed with accurate quantitative nitrogen management (total nitrogen was 270 kg hm-2, base fertilizer:tiller fertilizer:spike fertilizer = 3.5:3.5:3.0) at basic seedlings of 380×104 and 300×104 hm-2 as the control. Then dry direct seeding rice yield and quality were systematically determined and compared with the control and “solo-stalk” cultural method with nitrogen fertilizer in whole growth duration applied in middle and late tillering stage. The results showed that rice yield showed a trend of first increased and then decreased with nitrogen application at bigger leaf age. Rice yield was significantly higher than other treatments when applying nitrogen fertilizer at the 8-leaf stage, and the yield was further improved with the increase of nitrogen application amount. Compare with the controls, nitrogen fertilizer in whole growth duration of 180 kg N hm-2 applied one time at 8-leaf stage could significantly increase rice yield by 5.10% and 8.65%, and reduced nitrogen fertilizer by 33.3%, whereas nitrogen fertilizer in whole growth duration of 225 kg N hm-2 applied two time at 8-leaf stage and 7 days later could significantly increase rice yield by 7.46% and 11.09%, and reduced the nitrogen by 16.7%. The reason was that, compared with the control, seed setting rate and 1000-grain weight, effective panicle number was significantly increased resulting in the increasing total spikelet amount per hectare and yield on the basis of maintaining larger panicle type. With nitrogen applied at bigger leaf age, the head rice rate, chalkiness and protein content of rice revealed an increasing trend, but the amylose content and taste value of rice showed a decreasing trend. Compare to the two controls, the processing quality of rice with the head rice rate was increased by 0.67%-2.23% with nitrogen fertilizer in whole growth duration applied at 8-leaf age; the appearance quality was improved with the chalkiness decreased by 3.6%-14.5%; the nutrition quality was better with protein content increased by 3.03%-14.08%; the cooking and eating quality showed a tendency of getting better with amylose content decreased by 4.23%-10.95%; and there was no insignificant difference in taste value. In conclusion, nitrogen fertilizer in whole growth duration applied at suitable leaf age in the middle and late tillering stage could improve the quality and increase the yield of dry direct seeding rice under “solo-stalk” cultural method caused by late sowing dates, large sowing rate, and high basic seedlings in a rice-wheat cropping system. Keywords:“solo-stalk” cultivation mode;base fertilizer without nitrogen fertilizer;nitrogen fertilizer in middle and late tillering stage;nitrogen fertilizer management mode;direct seeding rice;yield;quality
PDF (712KB)元数据多维度评价相关文章导出EndNote|Ris|Bibtex收藏本文 本文引用格式 赵杰, 李绍平, 程爽, 田晋钰, 邢志鹏, 陶钰, 周磊, 刘秋员, 胡雅杰, 郭保卫, 高辉, 魏海燕, 张洪程. “独秆”栽培模式下全程氮肥在分蘖中后期施用对旱直播水稻产量和品质的影响[J]. 作物学报, 2021, 47(6): 1162-1174. doi:10.3724/SP.J.1006.2021.02052 ZHAO Jie, LI Shao-Ping, CHENG Shuang, TIAN Jin-Yu, XING Zhi-Peng, TAO Yu, ZHOU Lei, LIU Qiu-Yuan, HU Ya-Jie, GUO Bao-Wei, GAO Hui, WEI Hai-Yan, ZHANG Hong-Cheng. Effects of nitrogen fertilizer in whole growth duration applied in the middle and late tillering stage on yield and quality of dry direct seeding rice under “solo-stalk” cultivation mode[J]. Acta Agronomica Sinica, 2021, 47(6): 1162-1174. doi:10.3724/SP.J.1006.2021.02052
根据当地水稻换茬时间和及时抢播的要求, 于6月18日采用多功能一体化旱直播机(秸秆还田、施肥、耕整地、播种、镇压和开沟等多道工序一体化作业机)播种, 行距为25 cm, 播种量为195 kg hm-2, 播后进行小区规划, 调查基本苗并定苗至380×104 hm-2。
试验采用裂区设计, 以施氮量(N)为主区, 追肥叶龄期(D)为裂区, 设置2个氮肥水平, 纯氮分别为180 kg hm-2 (N1)和225 kg hm-2 (N2), 各氮肥水平下设置5个追肥叶龄期, 为六叶期(L6)、七叶期(L7)、八叶期(L8)、九叶期(L9)和十叶期(L10)。N1处理在各叶龄期一次性施用尿素150 kg hm-2和45%复合肥750 kg hm-2, N2处理在N1施肥的基础上7 d后追施尿素98 kg hm-2。同时, 在上述主体试验的基础上增设当地直播代表性生产模式对照CK1和CK2, 基本苗分别为380×104 hm-2和300×104 hm-2, 总施氮量270 kg hm-2, 按基肥∶蘖肥∶穗肥=3.5∶3.5∶3.0比例施用, 穗肥按促花肥(倒四叶)、保花肥(倒二叶)等量施入; 磷肥一次性基施, 钾肥分别于耕翻前, 促花肥等量施入。各小区基肥肥料于机械作业前撒施。具体氮、磷、钾肥用量及方式详见表1。
Table 1 表1 表1试验肥料用量和施用时期 Table 1Amount and application stage of fertilizer used in the study (kg hm-2)
氮肥 水平 N level
基肥 Base fertilizer
分蘖肥 Tiller fertilizer
叶龄追肥 Fertilization at leaf age
促花肥 Flower-promoting fertilizer
保花肥 Flower-preserving fertilizer
合计 Total
N
P2O5
K2O
N
N
P2O5
K2O
N
K2O
N
N
P2O5
K2O
N1
22.5
112.5
180
112.5
112.5
180
135
225
N2
22.5
112.5
225
112.5
112.5
225
135
225
CK1
94.5
135
112.5
94.5
40.5
112.5
40.5
270
135
225
CK2
94.5
135
112.5
94.5
40.5
112.5
40.5
270
135
225
N1处理在各叶龄期一次性施用尿素150 kg hm-2和45%复合肥750 kg hm-2; N2处理在N1施肥的基础上7 d后施用尿素98 kg hm-2。N1和N2处理基肥施用过磷酸钙37 kg hm-2和氯化钾179.5 kg hm-2。CK1和CK2施肥方式相同, 氮素以尿素施用, 磷钾肥分别以过磷酸钙和氯化钾施用。 In the N1 treatment, 150 kg hm-2 of urea and 45% compound fertilizer 750 kg hm-2 were applied at each leaf age; in N2 treatment, 98 kg hm-2 of urea was applied after 7 days on the basis of N1 fertilization. In the N1 and N2 treatments, 37 kg hm-2 of superphosphate and 179.5 kg hm-2 of potassium chloride were applied as the base fertilizer. CK1 and CK2 are applied in the same way, nitrogen is applied with urea, and phosphorus and potassium are applied with superphosphate and potassium chloride, respectively.
N1: 180 kg N hm-2; N2: 225 kg N hm-2; L6、L7、L8、L9、L10分别为六、七、八、九和十叶龄期5个追肥叶龄期。不同小写字母表示在0.05水平差异显著(LSD法)。 Fig. 2Effects of nitrogen fertilizer in whole growth duration applied in the middle and late tillering stage on yield of dry direct seed rice under “solo-stalk” cultivation mode in 2018 and 2019
N1: 180 kg N hm-2; N2: 225 kg N hm-2; L6, L7, L8, L9, and L10 are the five topdressing leaf ages of 6, 7, 8, 9, and 10, respectively. Different lowercase letters indicate significant differences at the 0.05 probability level by LSD test.
Table 2 表2 表2“独秆”栽培模式下全程氮肥在分蘖中后期施用对旱直播水稻产量构成因素的影响 Table 2Effects of nitrogen fertilizer in whole growth duration applied in the middle and late tillering stage on yield components of dry direct seed rice under “solo-stalk” cultivation mode
处理Treatment
基本苗 Basic seedlings (×104 hm-2)
有效穗数 Number of panicle (×104 hm-2)
每穗粒数 Spikelets per panicle
群体颖花量 Total spikelet number (×108 hm-2)
结实率 Seed setting rate (%)
千粒重 1000-grain weight (g)
氮肥水平 N level
追肥叶龄 Leaf age
N1
L6
381.8 a
409.8 e
85.6 cd
3.51 cd
95.66 a
27.2 a
L7
381.9 a
428.6 d
88.4 bc
3.78 c
93.85 ab
27.4 a
L8
382.0 a
455.3 b
93.7 a
4.29 ab
92.85 bc
27.4 a
L9
382.4 a
436.5 cd
83.5 d
3.69 cd
91.66 c
27.2 a
L10
382.8 a
446.2 bc
75.3 e
3.45 d
92.72 bc
27.1 a
N2
L6
382.1 a
429.3 d
88.0 bc
3.69 cd
95.46 a
27.3 a
L7
381.5 a
450.2 b
90.4 ab
4.09 b
94.17 ab
27.1 a
L8
383.2 a
474.2 a
94.5 a
4.54 a
92.59 bc
27.3 a
L9
382.3 a
449.8 b
82.0 d
3.79 c
91.31 c
27.2 a
L10
382.4 a
454.2 b
75.0 e
3.55 cd
92.21 bc
27.2 a
F-value
N
0.23NS
147.00**
1.25NS
32.28*
0.22NS
0.05NS
L
0.70NS
28.14**
59.11**
32.17**
25.96**
0.15NS
N×L
0.48NS
0.79NS
0.76NS
0.55NS
0.26NS
0.13NS
CK1
382.1
419.2
91.6
3.85
90.73
27.2
CK2
303.5
372.7
104.7
3.92
91.52
27.2
处理和缩写同图2。同一列数据不同小写字母表示在0.05水平差异显著(LSD法)。*和**分别表示在0.05和0.01水平差异显著, NS表示差异不显著。 Treatments and abbreviations are the same as those given in Fig. 2. Data followed by different lowercase letters in the same column indicate significant differences at the 0.05 probability level by LSD test. * and * * are significant differences at the 0.05 and 0.01 probability levels, respectively; “NS” is not significant difference.
Table 3 表3 表3“独秆”栽培模式下全程氮肥在分蘖中后期施用对旱直播水稻穗型结构的影响 Table 3Effects of nitrogen fertilizer in whole growth duration applied in the middle and late tillering stages on panicle structure of dry direct seed rice under “solo-stalk” cultural code
处理Treatment
整穗Panicle
一次枝梗Primary branches
二次枝梗Secondary branches
氮肥水平 N level
追肥叶龄 Leaf age
穗长 PL (cm)
着粒密度 GD (grain cm-1)
单穗重 GWPP (g)
一二次枝梗比 RN
一二次枝梗总粒数比 RTG
总粒数 TG
枝梗数 NB
单枝梗着粒数 GPB
总粒数 TG
结实率 SSR (%)
枝梗数 NB
单枝梗着粒数 GPB
总粒数 TG
结实率 SSR (%)
N1
L6
13.8 ab
6.21 de
2.17 c
0.75 a
1.53 a
85.6 c
9.0 a
5.72 a
51.7 a
97.7 b
12.1 de
2.81 b
33.9 d
88.0 b
L7
13.7 ab
6.44 bc
2.20 bc
0.64 cd
1.34 bc
88.4 b
8.5 b
5.94 a
50.5 a
97.4 b
13.3 bc
2.85 b
37.9 bc
87.0 c
L8
13.6 b
6.87 a
2.36 a
0.56 f
1.16 de
93.7 a
8.4 b
6.00 a
50.2 a
96.6 c
14.9 a
2.91 ab
43.5 a
85.9 d
L9
13.7 ab
6.09 e
2.11 d
0.61 e
1.28 cde
83.7 cd
7.8 c
5.99 a
46.9 b
96.6 c
13.0 c
2.84 b
36.8 bcd
87.7 b
L10
13.9 ab
5.39 g
1.91 e
0.74 ab
1.55 a
75.2 e
7.7 c
5.96 a
45.7 bc
97.0 c
10.4 g
2.84 b
29.5 e
88.2 b
N2
L6
14.0 a
6.29 cd
2.20 bc
0.73 ab
1.44 abc
88.1 b
9.1 a
5.74 a
52.0 a
98.3 a
12.4 d
2.92 ab
36.2 cd
88.2 b
L7
13.9 ab
6.49 b
2.24 b
0.63 de
1.28 cde
90.0 b
8.4 b
5.87 a
50.6 a
97.6 b
13.4 b
2.94 ab
39.5 b
86.4 cd
L8
13.9 ab
6.86 a
2.38 a
0.57 f
1.11 e
95.1 a
8.3 b
6.02 a
50.1 a
97.5 b
14.6 a
3.09 a
45.1 a
84.9 e
L9
13.9 ab
5.92 f
2.07 d
0.66 c
1.32 bcd
82.2 d
7.8 c
5.99 a
46.6 bc
97.6 b
11.8 e
3.01 ab
35.6 cd
84.6 e
L10
13.9 ab
5.40 g
1.93 e
0.71 b
1.45 ab
74.8 e
7.7 c
5.73 a
44.3 c
97.8 b
10.8 f
2.82 b
30.6 e
89.2 a
F-value
N
9.9NS
1.27NS
9.15NS
0.55NS
2.59NS
5.14NS
0.13NS
0.25NS
0.27NS
80.18*
4.07NS
8.07NS
3.14NS
171.26**
L
0.88NS
147.44**
183.93**
109.43**
29.80**
169.64**
57.35**
5.97**
28.35**
33.59**
339.47**
3.77*
105.76**
73.40**
N×L
0.96NS
1.2NS
1.41NS
4.99**
0.96NS
2.2NS
0.14NS
1.32NS
0.40NS
6.44**
14.76**
1.37NS
1.71NS
22.74**
CK1
13.9
6.60
2.22
0.67
1.42
91.7
9.0
5.99
53.8
94.05
13.4
2.83
37.9
85.04
CK2
14.2
7.37
2.48
0.63
1.31
104.7
9.9
6.00
59.3
95.28
15.7
2.90
45.4
86.51
处理和缩写同图2。同一列数据不同小写字母表示在0.05水平下差异显著(LSD法),*和**分别表示在0.05和0.01水平差异显著,NS表示差不异显著。 Treatments and partial abbreviations are the same as those given in Fig. 2. PL: panicle length; GD: grain density; GWPP: grain weight per panicle; RN: ratio of No. of branches of primary branches to No. of branches of secondary branches; RTG: ratio of total grains of primary branches to total grains of secondary branches; NB: Number of branches; GPB: grains per branch; TG: total grains; SSR: seed-setting rate. Data followed by different lowercase letters in the same column indicate significant differences at the 0.05 probability level by LSD test. * and * * are significant differences at the 0.05 and 0.01 probability levels, respectively. The “NS” was not significant difference.
Table 4 表4 表4“独秆”栽培模式下全程氮肥在分蘖中后期施用对旱直播水稻茎蘖动态的影响 Table 4Effects of nitrogen fertilizer in whole growth duration applied in the middle and late tillering stages on dynamics of tiller of dry direct seed rice under “solo-stalk” cultivation mode (×104 hm-2)
处理Treatment
六叶期 6-leaf stage
十叶期 10-leaf stage
拔节期 Jointing stage
抽穗期 Heading stage
成熟期 Maturity stage
氮肥水平 N level
追肥叶龄 Leaf age
N1
L6
426.7 a
766.7 a
574.7 d
428.0 e
409.8 e
L7
423.4 a
566.0 b
689.4 ab
474.0 bc
428.6 d
L8
420.0 a
541.4 b
626.0 cd
489.4 b
455.3 b
L9
420.7 a
542.7 b
578.0 d
458.7 cd
436.5 cd
L10
421.4 a
542.0 b
575.4 d
449.4 cde
446.2 bc
N2
L6
425.4 a
801.4 a
601.4 cd
434.7 de
429.3 d
L7
423.4 a
568.0 b
724.0 a
490.7 b
450.2 b
L8
420.0 a
542.0 b
646.7 bc
514.7 a
474.2 a
L9
422.0 a
538.7 b
581.4 d
470.7 bc
449.8 b
L10
426.7 a
544.0 b
583.4 d
456.0 cd
454.2 b
F-value
N
0.84NS
1.28NS
25.69*
29.23*
147.00**
L
0.79NS
84.57**
61.33**
22.29**
28.14**
N×L
0.25NS
0.46NS
0.86NS
0.47NS
0.79NS
CK1
529.4
679.7
545.4
430.0
419.2
CK2
340.7
556.0
393.4
381.4
372.7
处理和缩写同图2。同一列数据不同小写字母表示在0.05水平差异显著(LSD法)。*和**分别表示在0.05和0.01水平差异显著, NS表示差异不显著。 Treatments and abbreviations are the same as those given in Fig. 2. Data followed by different lowercase letters in the same column indicate significant differences at the 0.05 probability level by LSD test. * and * * are significant difference at the 0.05 and 0.01 probability levels, respectively; “NS” is not significant difference.
处理和缩写同图2。不同小写字母表示在0.05水平差异显著(LSD法)。 Fig. 3Effects of nitrogen fertilizer in whole growth duration applied in the middle and late tillering stages on percentage of productive tillers and stems of dry direct seed rice under “solo-stalk” cultivation mode
Treatments and abbreviations are the same as those given in Fig. 2. Data followed by different lowercase letters indicate significant differences at the 0.05 probability level by LSD test.
Table 5 表5 表5“独秆”栽培模式下全程氮肥在分蘖中后期施用对旱直播水稻加工品质和外观品质的影响 Table 5Effects of nitrogen fertilizer in whole growth duration applied in the middle and late tillering stages on processing and appearance quality of dry direct seed rice under “solo-stalk” cultivation mode
处理Treatment
加工品质Processing quality
外观品质Appearance quality
氮肥水平 N level
追肥叶龄 Leaf age
糙米率 BRR (%)
精米率 MRR (%)
整精米率 HMR (%)
长宽比 LWR
透明度 TD
垩白大小 CS (%)
垩白粒率 CGR (%)
垩白度 CD (%)
N1
L6
84.67 a
66.18 a
62.19 a
1.65 a
3.0 a
56.9 a
9.3 a
5.3 f
L7
84.76 a
66.36 a
62.66 a
1.66 a
3.0 a
60.5 a
9.2 a
5.5 ef
L8
84.86 a
66.54 a
63.15 a
1.66 a
3.0 a
59.0 a
11.1 a
6.4 cde
L9
84.96 a
66.68 a
63.89 a
1.66 a
3.0 a
61.6 a
11.7 a
7.2 abc
L10
85.30 a
67.15 a
64.29 a
1.67 a
3.0 a
67.8 a
11.4 a
7.8 ab
N2
L6
84.66 a
66.38 a
62.40 a
1.66 a
3.0 a
61.2 a
9.6 a
5.5 ef
L7
84.76 a
66.97 a
63.01 a
1.66 a
3.0 a
62.1 a
9.6 a
5.9 def
L8
85.13 a
67.11 a
63.74 a
1.66 a
3.0 a
60.2 a
11.6 a
7.0 bcd
L9
85.20 a
67.52 a
64.01 a
1.66 a
3.0 a
63.9 a
12.1 a
7.7 ab
L10
85.30 a
68.04 a
64.64 a
1.67 a
3.0 a
70.3 a
11.7 a
8.2 a
F-value
N
1.13NS
0.59NS
0.29NS
0.07NS
—
0.60NS
0.42NS
8.41NS
L
1.66NS
1.03NS
3.68NS
1.14NS
—
2.94NS
3.61*
22.50**
N×L
0.14NS
0.08NS
0.04NS
0.05NS
—
0.06NS
0.01NS
0.05NS
CK1
84.28
66.34
62.35
1.67
3.0
71.5
10.5
7.5
CK2
84.88
66.72
62.72
1.67
3.0
65.2
11.1
7.2
处理和缩写同图2。同一列数据不同小写字母表示在0.05水平差异显著(LSD法)。*和**分别表示在0.05和0.01水平差异显著, NS表示差异不显著。 Treatments and partial abbreviations are the same as those given in Fig. 2. BRR: brown rice rate; MRR: milled rice rate; HMR: head milled rice rate; LWR: length width rate; TD: transparent degree; CS: chalkiness size; CGR: chalkiness grain rate; CD: chalkiness degree. Data followed by different lowercase letters in the same column indicate significant differences at the 0.05 probability level by LSD test . * and * * are significant difference at the 0.05 and 0.01 probability levels, respectively; “NS” is not significant difference.
Table 6 表6 表6“独秆”栽培模式下全程氮肥在分蘖中后期施用对旱直播水稻蒸煮食味品质和营养品质的影响 Table 6Effects of nitrogen fertilizer in whole growth duration applied in the middle and late tillering stage on cooking, eating and nutritional quality of dry direct seed rice under “solo-stalk” cultivation mode
处理Treatment
食味值 Taste value
外观 Appearance
硬度 Hardness
黏度 Viscosity
蛋白质含量 PC (%)
直链淀粉含量 AC (%)
氮肥水平 N level
追肥叶龄 Leaf age
N1
L6
82.1 a
8.3 a
5.8 e
8.7 a
7.63 e
12.49 a
L7
78.7 a
7.8 a
6.0 d
8.3 a
7.73 de
12.08 ab
L8
74.0 bc
7.1 bc
6.3 bc
7.6 b
7.93 de
11.69 abc
L9
73.8 bc
7.0 bc
6.4 bc
7.6 b
8.13 cde
11.10 bcd
L10
70.8 cd
6.5 cd
6.6 a
7.2 bc
8.28 bcde
10.42 d
N2
L6
81.4 a
8.2 a
5.8 e
8.6 a
7.98 cde
12.15 ab
L7
74.8 b
7.2 b
6.2 cd
7.6 b
8.35 bcd
11.52 abc
L8
73.2 bc
7.0 bc
6.3 bc
7.4 bc
8.62 abc
11.13 bcd
L9
70.8 cd
6.6 cd
6.5 ab
7.1 bc
8.83 ab
10.64 cd
L10
68.7 d
6.3 d
6.7 a
6.8 c
9.13 a
10.15 d
F-value
N
17.27NS
12.98NS
3.27NS
16.69NS
132.13**
51.64*
L
39.29**
38.67**
45.57**
28.14**
5.90**
10.00**
N×L
0.89NS
1.03NS
0.44NS
1.15NS
0.39NS
0.07NS
CK1
72.0
7.0
6.4
7.2
7.70
12.20
CK2
74.1
7.2
6.1
7.6
7.55
12.50
处理和缩写同图2。同一列数据不同小写字母表示在0.05水平差异显著(LSD法)。*和**分别表示在0.05和0.01水平差异显著, NS表示差异不显著。 Treatments and abbreviations are the same as those given in Fig. 2. PC: protein content; AC: amylose content. Data followed by different lowercase letters in the same column indicate significant differences at the 0.05 probability level by LSD test. * and * * are significant difference at the 0.05 and 0.01 probability levels, respectively; “NS” is not significant difference.
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Huang YY, Zhao SS, Fu YC, Sun HD, MaX, Tan LB, Liu FX, Sun XY, Sun HY, GuP, Xie DX, Sun CQ, Zhu ZF. Variation in the regulatory region of FZP causes increases in secondary inflorescence branching and grain yield in rice domestication , 2018,96:716-733. DOI:10.1111/tpj.14062URLPMID:30101570 [本文引用: 1] Inflorescence branching is a key agronomic trait determining rice yield. The primary branch of the ancestral wild rice (Oryza rufipogon Griff.) bears few grains, due to minimal secondary branching. By contrast, Oryza sativa cultivars have been selected to produce large panicles with more secondary branches. Here we showed that the CONTROL OF SECONDARY BRANCH 1 (COS1) gene, which is identical to FRIZZY PANICLE (FZP), plays an important role in the key transition from few secondary branches in wild rice to more secondary branches in domesticated rice cultivars. A 4-bp tandem repeat deletion approximately 2.7 kb upstream of FZP may affect the binding activities of auxin response factors to the FZP promoter, decrease the expression level of FZP and significantly enhance the number of secondary branches and grain yield in cultivated rice. Functional analyses showed that NARROW LEAF 1 (NAL1), a trypsin-like serine and cysteine protease, interacted with FZP and promoted its degradation. Consistently, downregulating FZP expression or upregulating NAL1 expression in the commercial cultivar Zhonghua 17 increased the number of secondary branches per panicle, grain number per panicle and grain yield per plant. Our findings not only provide insights into the molecular mechanism of increasing grain number and yield during rice domestication, but also offer favorable genes for improving the grain yield of rice.
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