田宇,
张帅,
孙林,
北京师范大学生命科学学院，北京师范大学北京市抗性基因资源与分子发育重点实验室， 100875，北京
基金项目:国家自然科学基金资助项目 (31871243)；北京师范大学自主科研学科交叉重大资助项目 (310421101)
详细信息
通讯作者:孙林（1978—），男，副教授，博士，博士生导师. 研究方向：分子遗传学和基因组学. E-mail：sunlin@bnu.edu.cn
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出版历程
收稿日期:2020-11-03
网络出版日期:2021-01-21
刊出日期:2021-06-30
RNA expression analysis of MSL complex components in Drosophila aneuploid embryogenesis
Haizhu QI,Yu TIAN,
Shuai ZHANG,
Lin SUN,
College of Life Sciences, Beijing Key Laboratory of Gene Resource and Molecular Development, Beijing Normal University, 100875, Beijing, China
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摘要
摘要:MSL (male specific lethal) 复合体作为调节果蝇剂量补偿的关键元件，在非整倍体基因表达调控过程中发挥了重要作用．为深入探究非整倍体胚胎发育过程的分子调控机制，运用TSA-FISH (tyramide signal amplification- fluorescence in situ hybridization) 技术比较分析正常二倍体胚胎与常染色体三体胚胎中的MSL复合体组分基因表达模式，探究复合体重要组分在果蝇非整倍体胚胎不同时期的表达水平，以及亚细胞定位的变化．研究结果表明，在MSL复合体尚未组装的非整倍体胚胎发育早期，即母体表达阶段，多数复合体组分基因就已存在转录本水平上的差异，而这种表达差异主要源自于母体的非单倍体配子．随着发育的进行，染色体片段的增加导致基因组内剂量平衡发生变化，由此产生的反式剂量效应将引发MSL复合体各组分表达水平的差异变化，而这种差异将持续作用于后续非整倍体胚胎发育的各个时期．
关键词:非整倍体/
MSL复合体/
TSA-FISH/
反式剂量效应
Abstract:Unbalanced gene dosage of aneuploidy usually results in some detrimental consequences in development, or even in death．The male specific lethal (MSL) complex, which is thought to play a fundamental role in regulating dosage compensation in Drosophila , also has great impact on gene expression in aneuploidy．To explore the molecular mechanism in embryogenesis, gene expression of MSL complex components was compared between normal diploid and autosomal trisomy embryos．TSA-FISH (tyramide signal amplification-fluorescence in situ hybridization) technology was used to detect changes in subcellular localization of transcripts. In early stage of aneuploid embryo development or maternal expression stage when no MSL complex is assembled, most complex component genes already showed differences at the transcript level．Such differences mainly derived from maternal non-haploid gametes．As embryo development proceeds, gene dosage imbalance due to increased chromosome fragmentation produced inverse dosage effect leading to differential expression of the MSL complex components．Consequently, it will continue to affect subsequent development of aneuploid embryos at later stages．
Key words:aneuploidy/
MSL complex/
TSA-FISH/
inverse dosage effect
