副教授


郭学武，男，工学博士，副研究员，硕士生导师，天津市“131”人才计划第三层次人选。中国微生物学会会员。
教育经历：
2010/9 - 2014/6，天津大学，应用化学，工学博士
2002/9 - 2006/3，中国科学院微生物研究所，生物化学与分子生物学专业，硕士
1998/9 - 2002/6，湖南师范大学，生物科学，学士
工作经历：
2014/12 -至今，天津科技大学，生物工程学院，副研究员
2009/12 -2014/11，天津科技大学，生物工程学院，助理研究员
2006/8 - 2009/11，天津科技，生物工程学院，研究实习员
主讲课程：
本科生：发酵过程检测与控制；研究生：生物过程检测与控制
科研领域及方向
[1]现代酿造技术与食品安全；[2]生物基化学品与合成生物学；[3]酵母生物学
科研情况
主持并参与国家级和省部级等项目十余项，主持的
[1] 国家自然科学基金项目：高核酸酿酒酵母RNA合成的分子遗传机制研究，**、2017 -2020，主持
[2] 国家自然科学基金项目：全局转录因子σ定向进化提高肺炎克雷伯氏菌发酵木糖生产2,3-丁二醇能力的研究及其调控机理，**、2015-2017 ，主持
[3] 中国博士后科学基金面上项目：放线菌在白酒生产中的作用及其机制研究，2017M611169，2017-2019
[4] 天津市应用基础及前沿技术研究计划： 适量高产酯酿酒酵母优良基因工程菌种的选育，12JCQNJC06500、2012 -2015，主持
[5]国家科技支撑项目子课题：食品安全朔源控制及预警技术研究与推广应用示范---酵母安全生产与质量控制，主持
[6] 横向课题：安徽文王酿酒股份有限公司菌种鉴定与相关标准制定，主持
主要学术成果
先后发表各类科研论文70篇，参编教材或专著2部，授权或申请发明专利20项。发表的主要学术论文有：
1.Guo, XW*; Zhang, Y; Li, LL; Guo, J; Wu, DG; Chen, YF; Xiao, DG, Improved xylose tolerance and 2,3-butanediol production of Klebsiella pneumoniae by directed evolution of rpoD and the mechanisms revealed by transcriptomics, Biotechnol Biofuels, 2018,11:307 doi: 10.1186/s13068-018-1312-8
2.Li P, Gao Y, Wang C, Zhang CY, Guo X, Xiao D. Effect of ILV6 Deletion and Expression of aldB from Lactobacillus plantarum in Saccharomyces uvarum on Diacetyl Production and Wine Flavor. J Agric Food Chem. 2018,66(32):8556-8565. doi: 10.1021/acs.jafc.8b02356.
3.Li F, Yin C, Sun L, Li Y, Guo X, Song H. Synthetic Klebsiella pneumoniae-Shewanella oneidensis Consortium Enables Glycerol-Fed High-Performance Microbial Fuel Cells. Biotechnol J. 2018;13(5):e**. doi: 10.1002/biot..
4.Guo J, Huang S, Chen Y, Guo X, Xiao D. Heterologous expression of Spathaspora passalidarum xylose reductase and xylitol dehydrogenase genes improved xylose fermentation ability of Aureobasidium pullulans. Microb Cell Fact. 2018;17(1):64. doi: 10.1186/s12934-018-0911-1.
5.Li W, Chen SJ, Wang JH, Zhang CY, Shi Y, Guo XW, Chen YF, Xiao DG. Genetic engineering to alter carbon flux for various higher alcohol productions by Saccharomyces cerevisiae for Chinese Baijiu fermentation. Appl Microbiol Biotechnol. 2018; 102(4):1783-1795. doi: 10.1007/s00253-017-8715-5.
6.Guo J, Huang S, Chen Y, Guo X, Xiao D. Discovering the role of the apolipoprotein gene and the genes in the putative pullulan biosynthesis pathway on the synthesis of pullulan, heavy oil and melanin in Aureobasidium pullulans. World J Microbiol Biotechnol. 2018;34(1):11. doi: 10.1007/s11274-017-2398-z.
7.Guo, XW*; Wang, YZ; Guo, J; Wang, Q; Zhang, Y ; Chen, YF; Zhang, CY; Xiao, DG, Efficient production of 2,3-butanediol from cheese whey powder (CWP) solution by Klebsiella pneumoniae through integrating pulsed fed-batch fermentation with a two-stage pH control strategy, Fuel, 2017, 203: 469-477 DOI: 10.1016/j.fuel.2017.04.138
8.Li P, Guo X*, Shi T, Hu Z, Chen Y, Du L, Xiao D*. Reducing diacetyl production of wine by overexpressing BDH1 and BDH2 in Saccharomyces uvarum. J Ind Microbiol Biotechnol. 2017;44(11):1541-1550. doi: 10.1007/s10295-017-1976-2.
9.Shi TT, Li P, Chen SJ, Chen YF, Guo XW*, Xiao DG*. Reduced production of diacetyl by overexpressing BDH2 gene and ILV5 gene in yeast of the lager brewers with one ILV2 allelic gene deleted. J Ind Microbiol Biotechnol. 2017;44(3):397-405. doi: 10.1007/s10295-017-1903-6.
10.Song L, Chen Y, Du Y, Wang X, Guo X, Dong J, Xiao D. Saccharomyces cerevisiae proteinase A excretion and wine making. World J Microbiol Biotechnol. 2017;33(11):210. doi: 10.1007/s11274-017-2361-z.
11.Guo J, Wang Y, Li B, Huang S, Chen Y, Guo X, Xiao D. Development of a one-step gene knock-out and knock-in method for metabolic engineering of Aureobasidium pullulans. J Biotechnol. 2017, 251:145-150. doi: 10.1016/j.jbiotec.2017.04.029.
12.Yu H, Guo J, Chen Y, Fu G, Li B, Guo X, Xiao D. Efficient utilization of hemicellulose and cellulose in alkali liquor-pretreated corncob for bioethanol production at high solid loading by Spathaspora passalidarum U1-58. Bioresour Technol. 2017;232:168-175. doi: 10.1016/j.biortech.2017.01.077.
13.Chen Y, Song L, Han Y, Liu M, Gong R, Luo W, Guo X, Xiao D. Decreased proteinase A excretion by strengthening its vacuolar sorting and weakening its constitutive secretion in Saccharomyces cerevisiae. J Ind Microbiol Biotechnol. 2017;44(1):149-159. doi: 10.1007/s10295-016-1868-x.
14.Guo XW, Li YZ, Guo J, Wang Q, Huang SY, Chen YF, Du LP, Xiao DG. Reduced production of ethyl carbamate for wine fermentation by deleting CAR1 in Saccharomyces cerevisiae. J Ind Microbiol Biotechnol. 2016;43(5):671-9. doi: 10.1007/s10295-016-1737-7.
15.Chen Y, Luo W, Gong R, Xue X, Guan X, Song L, Guo X, Xiao D. Improved ethyl caproate production of Chinese liquor yeast by overexpressing fatty acid synthesis genes with OPI1 deletion. J Ind Microbiol Biotechnol. 2016;43(9):1261-70. doi: 10.1007/s10295-016-1795-x.
16.Guo J, Li J, Chen Y, Guo X, Xiao D. Improving Erythritol Production of Aureobasidium pullulans from Xylose by Mutagenesis and Medium Optimization. Appl Biochem Biotechnol. 2016;180(4):717-727.
17.Shi, TT; Guo, XW ; Li, P; Zhou, Z; Xiao, DG, Diacetyl content reduction in industrial brewer's yeast through ILV2 disruption and BDH1 expression, European Food Research and Technology, 2016, 242(6): 919-926 DOI: 10.1007/s00217-015-2598-4
18.Zhang, CY; Li, W; Wang, DS; Guo, XW; Ma, LJ; Xiao, DG, Production of 2,3-butanediol by Enterobacter cloacae from corncob-derived xylose, Turkish Journal of Biology, 2016, 40(4): 856-865 DOI: 10.3906/biy-1506-66
19.Guo X, Guan X, Wang Y, Li L, Wu D, Chen Y, Pei H, Xiao D. Reduction of biogenic amines production by eliminating the PEP4 gene in Saccharomyces cerevisiae during fermentation of Chinese rice wine. Food Chem. 2015;178:208-11. doi: 10.1016/j.foodchem.2015.01.089.
20.Chen, YF; Huang, WL; Fu, GX; Guo, J; Liu, MM; Guo, XW; Xiao, DG, A genetic transformation protocol for the xylose-fermenting yeast Spathaspora passalidarum, Engineering in Life Sciences,2015, 15(5): 550-555 DOI: 10.1002/elsc.
21.Dong J, Xu H, Zhao L, Chen Y, Zhang C, Guo X, Hou X, Chen D, Zhang C, Xiao D. Enhanced acetate ester production of Chinese liquor yeast by overexpressing ATF1 through precise and seamless insertion of PGK1 promoter. J Ind Microbiol Biotechnol. 2014;41(12):1823-8. doi: 10.1007/s10295-014-1522-4.
22.Guo X, Cao C, Wang Y, Li C, Wu M, Chen Y, Zhang C, Pei H, Xiao D. Effect of the inactivation of lactate dehydrogenase, ethanol dehydrogenase, and phosphotransacetylase on 2,3-butanediol production in Klebsiella pneumoniae strain. Biotechnol Biofuels. 2014;7(1):44. doi: 10.1186/1754-6834-7-44
23.Guo XW, Zhang YH, Cao CH, Shen T, Wu MY, Chen YF, Zhang CY, Xiao DG. Enhanced production of 2,3-butanediol by overexpressing acetolactate synthase and acetoin reductase in Klebsiella pneumoniae. Biotechnol Appl Biochem. 2014;61(6):707-15. doi: 10.1002/bab.1217
24.Zhang CY, Peng XP, Li W, Guo XW, Xiao DG. Optimization of 2,3-butanediol production by Enterobacter cloacae in simultaneous saccharification and fermentation of corncob residue. Biotechnol Appl Biochem. 2014;61(5):501-9. doi: 10.1002/bab.1198.
25.Chen Y, Li F, Guo J, Liu G, Guo X, Xiao D. Enhanced ethyl caproate production of Chinese liquor yeast by overexpressing EHT1 with deleted FAA1. J Ind Microbiol Biotechnol. 2014;41(3):563-72. doi: 10.1007/s10295-013-1390-3.
26.Chen, YF; Guo, J ; Li,; Liu, MM; Zhang, XX; Guo, XW; Xiao, DG, Production of pullulan from xylose and hemicellulose hydrolysate by Aureobasidium pullulans AY82 with pH control and DL-dithiothreitol addition, Biotechnology and Bioprocess Engineering, 2014, 19(2): 282-288 DOI: 10.1007/s12257-013-0715-4
27.Chen, YF; Zhang, XX; Zhang, SJ; Qin, WJ; Guo, CH; Guo, XW; Xiao, DG,Enhanced enzymatic xylose/cellulose fractionation from alkaline liquor-pretreated corn cob by surfactant addition and separate fermentation to bioethanol, Turkish Journal of Biology, 2014,38(4): 478-484 DOI: 10.3906/biy-1310-16
28.Zou J, Guo X, Shen T, Dong J, Zhang C, Xiao D. Construction of lactose-consuming Saccharomyces cerevisiae for lactose fermentation into ethanol fuel. J Ind Microbiol Biotechnol. 2013;40(3-4):353-63. doi: 10.1007/s10295-012-1227-5.
29.Wu D, Guo X, Lu J, Sun X, Li F, Chen Y, Xiao D. A rapid and efficient one-step site-directed deletion, insertion, and substitution mutagenesis protocol. Anal Biochem. 2013;434(2):254-8. doi: 10.1016/j.ab.2012.11.028.
30.Dong BY, Chen YF, Zhao CC, Zhang SJ, Guo XW, Xiao DG. Simultaneous determination of furfural, acetic acid, and 5-hydroxymethylfurfural in corncob hydrolysates using liquid chromatography with ultraviolet detection. J AOAC Int. 2013;96(6):1239-44.
31.Zhang, JW; Zhang, CY; Dai, LH; Dong, J; Liu, YL; Guo, XW; Xiao, DG, Effects of overexpression of the alcohol acetyltransferase-encoding gene ATF1 and disruption of the esterase-encoding gene IAH1 on the flavour profiles of Chinese yellow rice wine, International Journal of Food Science & Technology, 2012, 47(12): 2590-2596 DOI: 10.1111/j.1365-2621.2012.03140.x
32.Guo, XW; Wang, RS ; Chen, YF; Xiao, DG, Intergeneric yeast fusants with efficient ethanol production from cheese whey powder solution: Construction of a Kluyveromyces marxianus and Saccharomyces cerevisiae AY-5 hybrid, Engineering in Life Sciences, 2012,12(6): 656-661 DOI: 10.1002/elsc.
33.Lu, J; Dong, J; Wu, DG; Chen, YF; Guo, XW; Shi, Y; Sun, X; Xiao, DG, Construction of recombinant industrial brewer's yeast with lower diacetyl production and proteinase A activity, European Food Research and Technology, 2012, 235(5): 951-961 DOI: 10.1007/s00217-012-1821-9
34.Chen Y, Yang X, Zhang S, Wang X, Guo C, Guo X, Xiao D. Development of Saccharomyces cerevisiae producing higher levels of sulfur dioxide and glutathione to improve beer flavor stability. Appl Biochem Biotechnol. 2012;166(2):402-13. doi: 10.1007/s12010-011-9436-3.
35.Guo X, Zhou J, Xiao D. Improved ethanol production by mixed immobilized cells of Kluyveromyces marxianus and Saccharomyces cerevisiae from cheese whey powder solution fermentation. Appl Biochem Biotechnol. 2010;160(2):532-8. doi: 10.1007/s12010-008-8412-z.
36.Xiao D, Wu S, Zhu X, Chen Y, Guo X. Effects of soya fatty acids on cassava ethanol fermentation. Appl Biochem Biotechnol. 2010;160(2):410-20. doi: 10.1007/s12010-008-8344-7.
联系方式
办公地点：天津经济技术开发区第十三大街29号天津科技大学生物工程学院，
邮政编码：300457
办公电话：
传 真：
Email： guoxuewu@tust.edu.cn