jscheng@tju.edu.cn
未公开
教授
工业微生物发酵过程中细胞生物噪音的基础研究

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大规模培养细胞群体效应(子课题)

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β-内酰胺类抗生素工业发酵的信号转导调控研究

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应用蛋白组技术研究β-内酰胺类抗生素工业发酵过程

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混菌体系细胞间信息交流

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面向低劣生物质利用的人工多细胞体系设计与构建

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信号肽介导次生代谢物生产的系统解析与人工调控

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辅因子介导人工混菌降解磺胺甲恶唑研究

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降解抗生素的人工混菌系统中物质和能量交流

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环脂肽多组分同系物生物合成的人工调节研究

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《鸭传染性浆膜炎二价灭活疫苗（1型RAf63株+2型RAf34株）成果转化
高产菌株利迪链霉菌和选育及发酵。

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乙醇胺提高微藻脂含量的用途

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一种提高利迪链素产量的方法

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一种生物降解磺胺甲恶唑的方法

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利用维生素C提高多粘类芽孢杆菌2,3-丁二醇产量的方法

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青霉菌细胞内外蛋白质组的提取

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一种利迪链霉菌发酵液中抗真菌活性物质的分离纯化方法

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一种产2,3-丁二醇的多粘类芽孢杆菌及用途
公开了一种高产菌株利迪链霉菌和选育及发酵，高产菌株利迪链霉菌(Streptomyces lydicus)E9 CGMCC No.3075的发酵方法是：(1)摇瓶、种子和发酵培养基的配制；(2)摇瓶培养；(3)种子培养；(4)发酵罐培养：将种子培养液接入装有发酵培养基的发酵罐中，在通气，搅拌，温度为25～35℃，pH为4～9的条件下，培养24～96小时，得到高产菌株利迪链霉菌E9发酵液；.....

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公开了乙醇胺提高微藻脂含量的用途，将乙醇胺加入到培养微藻的培养基中。实验证明，向培养微藻的培养基中加入乙醇胺时，总脂含量提高10.2％-15.2％，从脂肪酸的角度分析，加入乙醇胺后提高了油酸(18∶1)的含量，因此还提高了生物柴油的质量。充分说明了乙醇胺在利用微藻生产生物柴油领域有很大的用途。

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本发明公开了一种利用膜生物反应器提高粪产碱杆菌(Alcaligenes faecalis )和脱氮假单胞杆菌(Pseudomonas sp. denitrificans)混菌生物降解磺胺甲恶唑的方法，属于环境污染治理领域。本发明通过在降解磺胺甲恶唑过程中，利用中空纤维膜交换粪产碱杆菌和脱氮假单胞杆菌两个发酵罐中的发酵液，抑制磺胺甲恶唑的再次生成，促进磺胺甲恶唑的生物降解。

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本发明公开了一种利用维生素C提高多粘类芽孢杆菌2,3-丁二醇产量的方法，本发明采用连续添加还原剂的方式，有利于整个发酵过程中生物所处外部环境的氧化还原态势的维持，最终促进了发酵液中葡萄糖的消耗和乙偶姻的转化，提高了(R,R)-2,3-丁二醇的积累

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本发明公开了一种青霉菌细胞内外蛋白质组的提取，包括如下步骤：（1）细胞内蛋白的提取：在LB培养基中接种青霉菌，培养，选8～12个时间点，收集细胞，每份细胞在液氮中研磨成细胞粉末；悬浮于裂解缓冲液中，加入DNaseⅠ和RNaseA的水溶液，加入苯甲基磺酰化氟-异丙醇溶液，超声，离心收集上清液，与丙酮混合，放置；离心，将沉淀物冻干，得到细胞内蛋白组，测定蛋白含量；（2）细胞外蛋白的提取：利用本发明的方法可以方便有效的提取蛋白，为鉴定不同工业发酵条件下蛋白变化奠定了基础。

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本发明公开了一种产2,3-丁二醇的多粘芽孢杆菌及用途，本发明的菌株经形态特征及16S rDNA分子生物学鉴定为多粘芽孢杆菌(Paenibacillus polymyxa) CJX518，现保藏于中国微生物菌种保藏管理委员会普通微生物中心，保藏号为CGMCC 7096，属革兰氏阳性菌。本发明的菌株能实现代谢产物中2,3-丁二醇构型的转化。出发菌株具有较强的(R,R)-2,3-丁二醇生产能力；经高糖驯化处理后，驯化菌株表现出较强的meso-2,3-丁二醇生产能力。
Improving the bioremoval of sulfamethoxazole and alleviating cytotoxicity of its biotransformation by laccase producing syste

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Simultaneous removal of ciprofloxacin, norfloxacin, sulfamethoxazole by co-producing oxidative enzymes system of Phanerochaet

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Exogenous cofactors for the improvement of bioremoval and biotransformation of sulfamethoxazole by Alcaligenes faecalis

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The biodegradation of cefuroxime, cefotaxime and cefpirome by the synthetic consortium with probiotic Bacillus clausii and in

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Artificial consortium that produces riboflavin regulates distribution of acetoin and 2,3-butanediol by Paenibacillus polymyxa

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Improvement of sulfamethoxazole (SMX) elimination and inhibition of formations of hydroxylamine-SMX

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Physiological responses and chromosomal aberration in root tip cells of Allium sativum L. to cerium treatments

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Regulation of extracellular oxidoreduction potential enhanced (R,R)-2,3-butanediol production by Paenibacillus polymyxa CJX51

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Release of proteins: Insights into oxidative response of Taxus cuspidata cells induced by shear stress

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Metabolic analysis reveals the amino acid responses of Streptomyces lydicus to pitching ratios during improving streptolydigin production

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ERK-like MAPK signaling and cytochrome c response to oleic acid in two-liquid-phase suspension cultures of Taxus cuspidata

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Xylose isomerase overexpression along with engineering of the pentose phosphate pathway and evolutionary engineering enable r

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Comparative proteome analysis of robust Saccharomyces cerevisiae insights into industrial continuous and batch fermentation

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Insights into the roles of exogenous glutamate and proline in improving streptolydigin production of Streptomyces lydicus with metabolomic analysis

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Inoculation-density-dependent responses and pathway shifts in Saccharomyces cerevisiae

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Expression of arabinogalactan proteins involved in Taxol production by immobilized Taxus cuspidata cells

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Comprehensive profiling of proteome changes provide insights of industrial Penicillium chrysogenum during pilot and industria

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Metabolome analysis reveals ethanolamine as potential marker for improving lipid accumulation of model photosynthetic organisms

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Medium optimization for antifungal active substance production from Streptomyces lydicus using response surface methodology

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Proteomic insights into adaptive responses of Saccharomyces cerevisiae to the repeated vacuum fermentation

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Investigation of proteomic responses of Streptomyces lydicus to pitching ratios for improving streptolydigin production

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Comparative Metabolomic Study of Penicillium chrysogenum During Pilot and Industrial Penicillin Fermentations

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Comparative metabolomic analysis on industrial continuous and batch ethanol fermentation processes by GC-TOF-MS

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Metabolome analysis of differential responses of diploid and haploid yeast to ethanol stress

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Transcriptome analysis of differential responses of diploid and haploid yeast to ethanol stress

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Genome-wide transcriptional analysis of Saccharomyces cerevisiae during industrial bioethanol fermentation

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Spatial-temporal distribution of nitric oxide involved in regulation of phenylalanine ammonia-lyase activation and taxol prod

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Inoculum size-dependent interactive regulation of metabolism and stress response of Saccharomyces cerevisiae revealed by comp

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混菌生物降解磺胺类药物的研究进展

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产黄青霉菌响应苯氧乙酸的Ca2+信号转导机制研究