姓名 尹海芳 职称 教授 所在
部门 细胞生物学系 研究
方向 基因治疗与药物运输
办公室 基础医学研究中心401 办公
电话 电子
邮箱 haifangyin@tmu.edu.cn
教育背景
1993.9-1997.7 内蒙古民族大学 农学 学士 农学
1997.9-2000.7 沈阳农业大学 理学 硕士 食品微生物
2000.9-2003.7 中国农业大学 理学 博士 生化与分子生物学

工作经历
2010.9-至今 天津医科大学 教授
2009.11-2011.11牛津大学Somerville学院 初级研究员（兼职）
2005.4-2010.8 牛津大学 博士后/课题组长
2004.4-2005.3 英国帝国理工大学 博士后
研究领域
主要从事基因治疗和药物运输，包括运动系统相关疾病和肝癌的治疗及药物运输研究。讲授主要课程包括：基因工程原理、医学细胞生物学、干细胞生物学和细胞信号转导分子机理及早期临床接触等本科生和研究生课程。

研究成果（本人具有代表性的论著、论文及主持的科研项目）
论著
及编
著 　

论文 　1.Xue Dong, Xianjun Gao, Yi Dai, Ning Ran, HaiFang Yin* .Serum exosomes can restore cellular function in vitro and be used for diagnosis in dysferlinopathy. (2017) Theranostics (Accepted).
　2.Zhen Lu, Bingfeng Zuo,Renwei Jing, Xianjun Gao, Quan Rao, Zhili Liu, Han Qi, Hua Guo, HaiFang Yin*. Dendritic cell-derived exosomes elicit tumor regression in autochthonous hepatocellular carcinoma mouse models. (2017) Journal of Hepatology 67(4): 739-748
　3.Gang Han, Ben Gu, Limin Cao, Xianjun Gao, Qingsong Wang, Yiqi Seow, Ning Zhang, Matthew JA Wood, HaiFang Yin*. Hexose enhances oligonucleotide delivery and exon-skipping in mdx mice. (2016) Nature Communications 7:10981.
　4.Limin Cao, Gang Han, Caorui Lin, Ben Gu, Xianjun Gao, Hong M Moulton, Yiqi Seow, HaiFang Yin*. Fructose promotes uptake and activity of oligonucleotides with different chemistries in a context-dependent manner in mdx mice. (2016) Molecular Therapy – Nucleic Acids 5(6):e329. doi: 10.1038/mtna.2016.46.
　5.Quan Rao, Bingfeng Zuo, Zhen Lu, Abin You, Xianjun Gao, Chenxuan Wu, Zhi Du, HaiFang Yin*. Tumor-derived exosomes elicit tumor suppression in murine hepatocellular carcinoma models and human in vitro. (2016) Hepatology 64(2):456-72. doi: 10.1002/hep.28549.
　6.Quan Rao, Abin You, Zhenglong Guo, Bingfeng Zuo, Xianjun Gao, Ti Zhang, Zhi Du, Chenxuan Wu, HaiFang Yin*.Intrahepatic Tissue Implantation Represents a Favorable Approach for Establishing Orthotopic Transplantation Hepatocellular Carcinoma Mouse Models. (2016) PLoS ONE 11(1):e**.
　7.Abin You, Manqing Cao, Zhigui Guo, Bingfeng Zuo, Junrong Gao, Hongyuan Zhou, Huikai Li, Yunlong Cui, Feng Fang, Wei Zhang, Tianqiang Song, Qixiang Li, Xiaolin Zhu, HaiFang Yin*, Huichuan Sun*, Ti Zhang*. Metformin sensitizes sorafenib to inhibit postoperative recurrence and metastasis of hepatocellular carcinoma in orthotopic mouse models. (2016) Journal of Hematology & Oncology 9:20. Doi: 10.1186/s13045-016-0253-6.
　8.Xianjun Gao, Xiaoyong Shen, Xue Dong, Ning Ran, Gang Han, Limin Cao, Ben Gu, HaiFang Yin*. Peptide Nucleic Acid Promotes Systemic Dystrophin Expression and Functional Rescue in Dystrophin-deficient mdx Mice. (2015) Molecular Therapy – Nucleic Acids 4: e255.
　9.Qingsong Wang, Gang Han, Jianhua Ye, Xianjun Gao, Hongjing Niu, Jingwen Zhao, Yawen Chai, Ning Li, HaiFang Yin*. Characterization of the polycystic kidney disease 2 gene promoter. (2014) Genomics 104(6Pt B):512-519.
　10.Limin Cao, Gang Han, Ben Gu, HaiFang Yin*. Wild-type mouse models to screen antisense oligonucleotides for exon-skipping efficacy in Duchenne muscular dystrophy. (2014) PLoS One9(11):e111079.
　11.Xianjun Gao, Jingwen Zhao, Gang Han, Yajie Zhang, Xue Dong, Limin Cao, Qingsong Wang, Hong M Moulton, HaiFang Yin*. Effective dystrophin restoration by a novel muscle-homing peptide –PMO conjugate in dystrophin-deficient mdx mice. (2014) Molecular Therapy 22(7): 1333-1341.
　12.Lu Yang, Hongjing Niu, Xianjun Gao, Qingsong Wang, Gang Han, Limin Cao, Chunquan Cai, Jan Weiler, HaiFang Yin*.Effective exon skipping and dystrophin restoration by 2’-O-methoxyethyl antisense oligonucleotide in dystrophin-deficient mice. (2013) PLoS One 8(4):e61584
　13.HaiFang Yin*, Prisca Boisguerin, Hong M Moulton, Corinne Betts, Yiqi Seow, Jordan Boutilier, Qingsong Wang, Anthony Walsh, Bernard Lebleu and Matthew JA Wood. Context dependence effects of chimeric peptide morpholino conjugates contribute to dystrophin exon-skipping efficiency. (2013) Molecular Therapy-Nucleic Acids 2: e124; doi:10.1038/mtna.2013.51.
　14.Corinne Betts, Suzan Hammond, HaiFang Yin, Matthew JA Wood. Optimizing tissue-specific antisense oligonucleotide-peptide conjugates. (2012) Methods Mol Biol. 867:415-35-38.
　15.Anthony S. Walsh, HaiFang Yin, Christoph M. Erben, Matthew Wood, Andrew J. Turberfield. DNA Cage delivery to Mammalian Cells. (2011) ACS Nano. 5(7):5427-32.
　16.HaiFang Yin, Amer F Saleh, Corinne Betts, Patrizia Camelliti, Yiqi Seow, Shirin Ashraf, Andrey Arzumanov, Michael Gait and Matthew Wood. Pip5 transduction peptides direct high efficiency oligonucleotide-mediated dystrophin exon skipping in heart and phenotypic correction in mdx mice. (2011) Molecular Therapy 19(7):1295-303. doi: 10.1038/mt.2011.79.
　17.Lydia Alvarez-Erviti, Yiqi Seow, HaiFang Yin, Corinne Betts, Samira Lakhal, Matthew Wood. Exosome-targeted systemic delivery of siRNA to the brain. Nature Biotechnology (2011) 29(4):341-5. doi:10.1038/nbt.1807.
　18.Qingsong Wang, HaiFang Yin, Jin He, Jianhua Ye, Fangrong Ding, Shaohua Wang, Xiaoxiang Hu,Qingyong Meng, Ning Li. cDNA cloning of porcine PKD2 gene and RNA interference in LLC–PK1 cells. (2011) Gene 476(1-2):38-45. Epub 2011 Jan 28.
　19.HaiFang Yin*, Hong M Moulton, Corinne Betts and Matthew Wood. CPP directed oligonucleotide exon skipping in animal models of Duchenne muscular dystrophy. (2011) Methods Mol Biol. 683:321-38, Springer/Humana Press.
　20.HaiFang Yin*, Alastair Crisp, Aurelie Goyenvalle, Corinne Betts, Hong M Moulton, Yiqi Seow, Arran Babbs, Thomas Merritt, Amer F. Saleh, Michael J Gait, Daniel J. Stuckey, Kieran Clarke, Kay E Davies and Matthew JA Wood. Diaphragm rescue alone prevents heart dysfunction in dystrophic mice. (2011) Human Molecular Genetics 20(3):413-21. Epub 2010 Nov 9.
　21.HaiFang Yin*, Hong M Moulton, Corinne Betts, Thomas Merritt, Yiqi Seow,Shirin Ashraf, QingSong Wang, Jordan Boutilier and Matthew Wood. Functional and phenotypic rescue of dystrophin-deficient mdx mice by a chimeric peptide-PMO oligomer. (2010) Molecular Therapy 18(10):1822-9, Epub 2010 August 10
　22.Yiqi Seow, HaiFang Yin, Matthew Wood. Identification of novel muscle targeting peptides in mdx mice. (2010) Peptides 31(10):1873-7, Epub 2010 Jul 16
　23.HaiFang Yin*, QingSong Wang, Patrizia Camelliti, Corinne Betts, Hong M Moulton, Hyunil Lee, Amer F Saleh, Michael J Gait and Matthew Wood. In vitro evaluation of novel antisense oligonucleotides is predictive of in vivo exon skipping activity for DMD.(2010) Journal of Gene Medicine 12(4):354-364, Epub March 16.
　24.Matthew Wood, Michael J Gait and HaiFang Yin. RNA-targted splice-correction therapy for neuromuscular disease. (2010) Brain 133(Pt4): 957-972, Epub 2010 Feb 11.
　25.HaiFang Yin*, Corinne Betts, Amer F Saleh, Gabriella Ivanova, Hyunil Lee, Yiqi Seow, Dalsoo Kim, Michael J Gait and Matthew Wood. Optimization of peptide nucleic acid antisense oligonucleotide for local and systemic dystrophin correction in mdx mouse.(2010) Molecular Therapy 18(4):819-827, Epub 2010 Jan 12.
　26.HaiFang Yin, Hong M Moulton, Corinne Boyd, Yiqi Seow, Jordan Boutilier, Patrick Iverson, Ryszard Kole and Matthew Wood. A muscle-targeting peptide domain directs systemic antisense oligonucleotide-induced splice correction of the DMD gene and functional restoration in dystrophin-deficient mdx mice. (2009) Human Molecular Genetics 18(22):4405-4414. Epub 2009 August 18.
　27.Matthew Wood, HaiFang Yin and Michael Gait. Muscle and heart targeted oligonucleotide splice correction for duchenne muscular dystrophy. (2009) Human Gene Therapy 20(11):1500-1501.
　28.Gabriela D. Ivanova, Andrey Arzumanov, Rachida Abes, HaiFang Yin, Matthew Wood, Bernard Lebleu and Michael J. Gait. Improved cell-penetrating peptide-PNA conjugates for splicing redirection in HeLa cells and exon skipping in mdx mouse muscle. (2008) Nucleic Acids Research 36(20):6418-28. Epub 2008 Oct 8.
　29.HaiFang Yin, Hong M. Moulton, Yiqi Seow, Corinne Boyd, Jordan Boutilier, Patrick Iverson and Matthew Wood. Cell-penetrating peptide conjugated antisense oligonucleotides restore systemic muscle and cardiac dystrophin expression and function. (2008) Human Molecular Genetics 17(24):3909-18. Epub 2008 Sep 10.
　30.HaiFang Yin, QiLong Lu, Matthew Wood. Effective exon skipping and restoration of dystrophin by peptide nucleic acid antisense oligonucletides in mdx mice. (2008) Molecular Therapy 16(1):38-45.
（#:第一作者；*:通讯作者）

科研
项目 　2017-2020 杜兴肌肉萎缩症（DMD）新型药物治疗及非人灵长类动物模型评价-课题负责人（国家重点研发计划 – 646万元）
　2017-2020新型生物纳米结构在dysferlin肌病靶向治疗的研究- 课题负责人（国家自然科学基金委-61万元）
　2014-2017新型运输辅助剂在杜兴肌肉萎缩症中的应用基础研究-课题负责人（天津市应用基础与前沿技术研究计划重点项目-20万元）
　2014-2016 中加健康研究合作计划项目：反义寡核苷酸介导外显子跳读在杜兴肌肉萎缩症转基因猪模型上的靶向治疗研究-课题负责人（国家自然科学基金委-100万元）
　2014-2017靶向心肌的反义寡核苷酸介导的多重外显子跳读在杜兴肌肉萎缩症上的研究-课题负责人（加拿大Jesse’s Journey- 10.5万加元）
　2013-2016新型生物纳米结构在肝细胞癌靶向治疗中的应用研究-课题负责人（国家自然科学基金委-65万元）
　2012-2016 肝炎等重大人类疾病的灵长类模型研制–课题负责人（国家重大科学研究计划目标导向课题-1134万元）
　2011-2013新型反义寡核酸药物在杜兴肌肉萎缩症基因治疗上的应用研究-课题负责人（国家自然科学基金委-37万元）

荣誉奖励
2017 天津市青年科技奖
2016 天津市“131”创新型人才团队带头人“药物运输创新团队”
2016 天津市****
2012 天津市“131”创新人才工程第一层次人选
2010 教育部新世纪优秀人才“新型靶向药物研发”

其他事项