水雯箐
性 别：女
职 称：副教授
所属部门：生物化学和分子生物学系
招生专业：生物化学和分子生物学
研究方向：细胞定量蛋白质组学，药物靶蛋白-小分子相互作用研究

::教师简介::
联系方式：email angelshui@nankai.edu.cnTel.**办公室地址：新生物站A202室 学历：2010年5月至今南开大学生命科学学院 副教授2009年至2010年 Thermo Fisher Scientific 生物技术公司美国总部质谱应用科学家 2004年至2008年 美国加州大学伯克利分校 化学系博士（化学生物学方向）1998年至2004年 复旦大学 化学系学士和硕士（分析化学方向）研究方向：当今的生命科学研究正逐步由定性、描述性、局部性研究，向精确、定量及整体化的方向发展，研究目光从原来注重特定分子，拓展到寻找生物系统的整体规律。定量生物学的飞跃式发展，离不开定量科学和技术的不断创新，其中生物质谱技术被公认为精确、定量、系统性研究生命体系所不可或缺的一项关键技术。质谱定量技术的每一步前进，都意味着对生命体系探测的深度和广度的进一步延伸。本课题组主要从事质谱定量分析技术的发展和生物学应用研究，建立了可精确测量复杂生命体系中的蛋白质和代谢物含量、翻译后修饰的定位和水平、蛋白质-小分子配体相互作用的技术路线。本课题组建立的膜蛋白质组定量分析方法为揭示细胞器的新功能、鉴定细胞特异的膜蛋白标志分子提供了关键技术；建立的无标质谱定量技术实现了对蛋白质翻译后修饰的定点定量的准确分析，为基于结构生物学的分子机制研究指明了方向；正在深入发展的定量检测靶蛋白-小分子相互作用的质谱新技术，有助于提高药物候选分子早期筛选的准确性和效率，也为系统性解析细胞内的酶蛋白与代谢物的互作网络开发出关键的支撑技术。研究成果在PNAS，Cell Research, Analytical Chemistry，Journal of Proteome Research等学术期刊上发表。如果你对发展新生物分析技术、从事分析化学-生物化学-细胞生物学的交叉前沿研究感兴趣，欢迎加入我们团队！主要论文发表：Chen X, Qin S, Chen S, Li J, Li L, Wang Z, Wang Q, Lin J, Yang C, Shui W*. A Ligand-observed Mass Spectrometry Approach Integrated into the Fragment Based Lead Discovery Pipeline. Scientific Reports 2015, 5, 8361.Zhang L, Liu S, Liu N, Zhang Y, Liu M, Li D, Seto E, Yao T, Shui W*, Zhou J*. Proteomic identification and functional characterization of MYH9, Hsc70, and DNAJA1 as novel substrates of HDAC6 deacetylase activity. Protein & Cell 2015, 6, 42-54.Chen J, Luo Y, Li L, Ran J, Wang X, Gao S, Liu M, Li D, Shui W*, Zhou J*. Phosphoregulation of the dimerization and functions of end-binding protein 1. Protein & Cell 2014, 5, 795–799.Wang Y, Li Z, Liu D, Xua J, Wei X, Yan L, Yang C, Lou Z, Shui W*. Assessment of BAK1 activity in different plant receptor-like kinase complexes by quantitative profiling of phosphorylation patterns. Journal of Proteomics, 2014, 108, 484-493.Liu S, Chen X, Yan Z, Qin S, Xu J, Lin J, Yang C, Shui W*. “Exploring Skyline for both MSE-based label free proteomics and HRMS quantitation of small molecules” Proteomics 2014, 14, 169-180.Chen X, Li L, Chen S, Xu Y, Xia Q, Guo Y, Liu X, Tang Y, Zhang T, Chen Y, Yang C*, Shui W*. “Identification of inhibitors of the antibiotic-resistance target NDM-1 using both nanoESI-MS- and ultrafiltration LC-MS-based approaches” Analytical Chemistry 2013, 85, 7957-7965. Xu J, Wei X, Yan L, Liu D, Ma Y, Guo Y, Peng C, Zhou H, Yang C, Lou Z, Shui W*. “Identification and Functional Analysis of Phosphorylation Residues of the Arabidopsis BOTRYTIS-INDUCED KINASE1” Protein Cell 2013, 10, 771-781.Hao J, Li W, Dan J, Ye X, Wang F, Zeng X, Wang L, Wang H, Cheng Y, Liu L*, Shui W*. "Reprogramming- and pluripotency-associated membrane proteins in mouse stem cells revealed by label-free quantitative proteomics"J Proteomics. 2013，86，70-84.Li Y, Wen T, Zhu M, Li L, Wei J, Wu X, Guo M, Liu S, Zhao H, Xia S, Huang W, Wang P, Wu Z, Zhao L, Shui W*, Li Z*, Yin Z*. "Glycoproteomic analysis of tissues from patients with colon cancer using lectin microarrays and nanoLC-MS/MS" Mol Biosyst. 2013, 9(7), 1877-87.Yan L, Ma Y, Liu D, Wei X, Sun Y, Chen X, Zhao H, Zhou J, Wang Z, Shui W*, Lou Z*. “Structural basis for the impact of phosphorylation on plant receptor-like kinase BAK1 activation” Cell Research 2012, 8 1304-1308.Shui, W.*; Lin, S.; Zhang, A.; Chen, Y.; Huang, Y.; Sanders, M. “Driving efficiency in a high-throughput metabolic stability assay through a generic high-resolution accurate mass method and automated data mining” Protein Cell 2011, 8, 680-688. Guo, Y.; Wang, J.; Niu, G.; Shui, W.; Sun, Y.; Zhou, H.; Zhang, Y.; Yang, C.; Lou, Z.; Rao, Z. “A structural view of the antibiotic degradation enzyme NDM-1 from a superbug” Protein Cell 2011, 5, 384-394.Shui, W.*; Petzold, C.; Redding, A.; Liu, J.; Pitcher, A.; Keasling, J.; Bertozzi, C. “Organelle Membrane Proteomics Reveals New Insights on Phagosome Maturation Mediated by Mycobacterial Lipoglycans” Journal of Proteome Research 2011, 10, 339-348. Shui, W.; Gilmore, S.; Sheu, L; Liu, J.; Keasling, J.; Bertozzi, C. “Quantitative Proteomic Profiling of Host-Pathogen Interactions: The Macrophage Response to Mycobacterium tuberculosis Lipids” Journal of Proteome Research 2009, 8, 282-289.Wu, P.; Shui, W.; Carlson, B.; Bertozzi, C. “Site-specific chemical modification of recombinant proteins produced in mammalian cells using the genetically-encoded aldehyde tag” Proceedings of the National Academy of Sciences 2009, 106, 3000-3005.Tang, Y.; Shui, W.; Myers, S.; Kang, Y.; Ouellet, M.; Bertozzi, C.; Keasling, J. “Central Metabolism and Hypoxic Response of Mycobacterium smegmatis Studied by Isotopomer Labeling” Biotechnology Letter 2009, 31, 1233-1240.(co-first)Shui W.; Sheu, L.; Liu, J.; Smart, B.; Keasling, J.; Bertozzi, C. “Membrane Proteomics of Phagosomes Suggests a Connection to Autophagy” Proceedings of the National Academy of Sciences 2008, 105, 16952-16957.Shui, W.; Fan, J.; Yang, P.; Liu, C.; Zhai, J.; Lei, J.; Yan, Y.; Zhao, D.; Chen, X. “Nanopore-based proteolytic reactor for sensitive and comprehensive proteomic analyses” Analytical Chemistry 2006, 78, 4811-4819.Shui, W.; Liu, Y.; Fan, H.; Bao, H.; Liang, S.; Yang, P.; Chen, X.“ Enhancing TOF/TOF-based de novo sequencing capability for high throughput protein identification with amino acid-coded mass tagging” Journal of Proteome Research 2005, 4, 83-90.Fan, J.; Shui, W.;Yang, P.; Wang, X.; Xu, Y.; Wang, H.; Chen, X.; Zhao, D. “Mesoporous silica nanoreactors for highly efficient proteolysis” Chemistry-A European Journal 2005, 11, 5391-5396.(co-first)
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