个人简介 高文运，博士，生物有机化学教授
通信地址：西北大学生命科学学院，西安市太白北路229号,邮编 710069
Tel: -852
Fax:
科研兴趣：
1、萜类化合物生物合成研究；
2、中草药有效成分的提取分离、结构鉴定及抗菌活性研究；
3、以萜类化合物生物合成的MEP途径中的酶为靶标的新抗菌素筛选。
教育经历
1995，9至1998，7
博士研究生，中国科学院上海药物研究所有机化学/天然产物化学专业
1985，9至1992，6
学士及硕士研究生，兰州大学化学系，分析化学专业
研究工作经历
2006，6至今
西北大学生命科学学院，萜类化合物MEP生物合成途径中的中间体研究
2003，11至2006，6
博士后研究，犹他大学化学系（美国），生物有机化学
Prof. C. Dale Poulter.
2000，9至 2003，10
洪堡奖学金及客座研究，马丁－路德大学生物中心（德国），生物有机化学
Prof. Dr. Meinhart H. Zenk.
1998，7至2000，7
博士后研究，中国科学院上海有机化学研究所，有机合成
林国强 院士，教授
1992，7至1995，8
兰州大学化学系，分析化学

承担的科研项目 科研项目：
1、萜类化合物MEP生物合成途径中的中间体转化过程及亚合成途径的研究，国家自然科学基金；
2、教育部留学回国人员科研启动项目。

主要研究成果 近期发表的论文：
1.Zhou, Y.F., Cui, Z., Li, H., Tian, J., Gao, Wenyun, Optimized enzymatic preparation of 1-deoxy-D-xylulose 5-phosphate. Bioorg. Chem. 2010. 38, 120-123.
2.Li, H., Tian, J., Wang, H., Yang, S.Q., Gao, Wenyun, An improved preparation of D-glyceraldehyde 3-phosphate and its use in the synthesis of 1-deoxy-D-xylulose 5-phosphate. Helv. Chim. Acta 2010, 93, 1745-1750.
3.Li, H., Yang, S.Q., Wang, H.,Tian, J., Gao, Wenyun, Biosynthesis of lamalbid in L. barbatum. Phytochemistry, 2010, 71, 1690-1694.
4.高文运, 同位素标记及未标记的1-脱氧-D-木酮糖-5-磷酸和2-甲基-D-赤藓糖醇-4-磷酸的合成方法，有机化学，2010，30，23-37。
5.Wenyun Gao, Yimin Li, Shanhao Jiang, Dayuan Zhu, Two lycopodium alkaloid N-oxides from Huperzia serrata, Helvetica Chimica Acta, 2008, 91, 1031-1035.
6.Wenyun Gao, R. Loeser, M. Raschke, M. A. Dessoy, M. Fulhorst, H. Alpermann, L. A. Wessjohann, M. H. Zenk, (E)-4-Hydroxy-3-methylbut-2-enyl diphosphate: an intermediate in the formation of terpenoids in plant chromoplasts. Angew. Chem., Int.l Ed., 2002, 41, 2604-2607.
7.U.-I. Flügge, Wenyun Gao, Transport of Isoprenoid Intermediates Across Chloroplast Envelope Membranes, Plant Biol (Stuttg) 2005, 7, 91-97.
8.Gao, Wenyun; Raschke, Maja; Alpermann, Henriette; Zenk, Meinhart H. A facile enzymatic synthesis of isotopically labeled 2-methyl-D-erythritol 2, 4-cyclo-diphosphate by spinach chloroplast stroma. Hel. Chim. Acta, 2003, 86, 3568-3577.
9.J. E. Page, G. Hause, M. Raschke, Wenyun Gao, J. Schmidt, M. H. Zenk, T. M. Kutchan, Functional Analysis of the Final Steps of the 1-Deoxy-D-xylulose 5-phosphate (DXP) Pathway to Isoprenoids in Plants Using Virus-Induced Gene Silencing, Plant Physiology, 2004, 134, 1401–1413.
10.Brandt, Wolfgang; Dessoy, Marco A.; Fulhorst, Michael; Gao, Wenyun; Zenk, Meinhart H.; Wessjohann, Ludger A. A proposed mechanism for the reductive ring opening of the cyclodiphosphate mecpp, a crucial transformation in the new DXP/MEP pathway to isoprenoids based on modeling studies and feeding experiments. ChemBioChem, 2004, 311-323.
11.高文运，李医明，蒋山好，朱大元， Three lycopodium alkaloid N-oxides from Huperzia serrata, Planta Med. 2000, 66: 664.
12.高文运，李医明，蒋山好，朱大元，A new lycopodium alkaloid from the whole plants of Huperzia serrata, Chin. J. Chem. 2000, 18: 614.
13.高文运，李医明，朱大元，Phenylic glycosides and -lactone glycoside from the sprouts of Crocus sativus,, Planta Med. 1999, 65: 425.
14.高文运，李医明，王保德，朱大元，Huperzine h, a new lycopodium alkaloid from Huperzia serrata, Chin. Chem. Lett., 1999, 10: 463.
15.李医明，蒋山好，高文运，朱大元，Iridoid glycosides from Scrophularia ningpoensis, Phytochemistry, 1999, 50: 101.
16.李医明，蒋山好，高文运，朱大元，Phenylpropanoid glycosides from Scrophularia ningpoensis, Phytochemistry, 2000, 54: 923.
近期申请专利：
1.高文运，李恒，田洁，廖娟，王雪娇，王辉，杨少青，化学法-酶法制备1-脱氧-D-木酮糖的方法，发明专利申请号：71。
2.高文运，李恒，田洁，王辉，杨少青，一种从植物提取物中筛选1-脱氧-D-木酮糖5-磷酸还原异构化酶抑制剂的方法，发明专利申请号：53。
3.高文运，田洁，廖娟，李恒，王学娇，戴少波，化学法-酶法制备1-脱氧-D-木酮糖5-磷酸的方法，发明专利申请号：49。
4.陈超，高文运，张腾霄，吩噻嗪类染料修饰的磁性微粒的制备方法和用途，申请号：3.7。
5.高文运，陈超，净化血液及血液制品的方法，申请号：5.6。
6.陈超，高文运，陈佳一，血红蛋白类氧载体冻干制剂的制备方法，申请号：1.4。
Dr. Wen-Yun Gao
Professor of Bioorganic/Organic Chemistry
College of Life Sciences, Northwest University
229 North Taibai Road, Xi’an, 710069, P. R. China
E-mail: gaowenyun@nwu.edu.cn
Tel: 86- ext. 852 (lab), Fax: 86-
RESEARCH INTERESTS:
?Biosynthesis of terpenoids by Mevonolate/MEP pathways
?Preparation of enzymes and enzyme-catalyzed reactions
?Isolation and structure elucidation of bioactive natural products
FUNDS:
?National Scientific Foundation of China
?Department of Education, China
RESEARCH, TEACHING AND WORK EXPERIENCE
July 2006?Present, Professor of bioorganic chemistry, College of Life Sciences, Northwest University
Nov. 2003?June 2006, Postdoctoral fellow, University of Utah, Bioorganic Chemistry
Mentor: Prof. C. Dale Poulter
Sept. 2000?Oct. 2003, Alexander von Humboldt research fellow and guest scientist,
Biocenter, Martin-Luther University, Germany, Bioorganic Chemistry
Mentor: Prof. Dr. M. H. Zenk
July, 1998?June, 2000, Research associate, Shanghai Institute of Organic Chemistry (SIOC), Chinese Academy of Sciences, Organic synthesis
Mentor: Prof. Guoqiang Lin
June, 1992?August, 1995, Lecturer, Lanzhou University, Analytical Chemistry
EDUCATION
?PhD, Organic Chemistry, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 1998
?BS/MS, Analytical Chemistry, Lanzhou University, 1985-1992
AWARDS/HONORS
?Alexander von Humboldt Scholarship from 2001 to 2003.
SELECTED PUBLICATIONS:
1.Zhou, Y.F., Cui, Z., Li, H., Tian, J., Gao, Wenyun, Optimized enzymatic preparation of 1-deoxy-D-xylulose 5-phosphate. Bioorg. Chem. 2010. 38, 120-123.
2.Li, H., Tian, J., Wang, H., Yang, S.Q., Gao, Wenyun, An improved preparation of D-glyceraldehyde 3-phosphate and its use in the synthesis of 1-deoxy-D-xylulose 5-phosphate. Helv. Chim. Acta 2010, 93, 1745-1750.
3.Li, H., Yang, S.Q., Wang, H.,Tian, J., Gao, Wenyun, Biosynthesis of lamalbid in L. barbatum. Phytochemistry, 2010, 71, 1690-1694.
4.Gao, Wenyun, Preparation of isotope labeled/unlabeled 1-deoxy-D-xylulose 5-phosphate and 2-methyl-D-erythreitol 4-phosphate, You Ji Hua Xue, 2010, 30, 23-37.
5.Wenyun Gao, Yimin Li, Shanhao Jiang, Dayuan Zhu, Two lycopodium alkaloid N-oxides from Huperzia serrata, Helvetica Chimica Acta, 2008, 91, 1031-1035.
6.Wenyun Gao, R. Loeser, M. Raschke, M. A. Dessoy, M. Fulhorst, H. Alpermann, L. A. Wessjohann, M. H. Zenk, (E)-4-Hydroxy-3-methylbut-2-enyl diphosphate: an intermediate in the formation of terpenoids in plant chromoplasts. Angew. Chem., Intl. Ed., 2002, 41, 2604-2607.
7.U.-I. Flügge, Wenyun Gao, Transport of Isoprenoid Intermediates Across Chloroplast Envelope Membranes, Plant Biol (Stuttg) 2005, 7, 91-97. (SCI)
8.Gao, Wenyun; M. Raschke; H. Alpermann; M. H. Zenk. A facile enzymatic synthesis of isotopically labeled 2-methyl-D-erythritol 2, 4-cyclo-diphosphate by spinach chloroplast stroma. Helv. Chim. Acta, 2003, 86, 3568-3577.
9.J. E. Page, G. Hause, M. Raschke, Wenyun Gao, J. Schmidt, M. H. Zenk, T. M. Kutchan, Functional Analysis of the Final Steps of the 1-Deoxy-D-xylulose 5-phosphate (DXP) Pathway to Isoprenoids in Plants Using Virus-Induced Gene Silencing, Plant Physiology, 2004, 134, 1401–1413.
10.Brandt, W.; Dessoy, M.A.; Fulhorst, M.; Gao, Wenyun; Zenk, M.H.; Wessjohann, L. A. A proposed mechanism for the reductive ring opening of the cyclodiphosphate mecpp, a crucial transformation in the new DXP/MEP pathway to isoprenoids based on modeling studies and feeding experiments. ChemBioChem, 2004, 311-323.
11.Wenyun Gao, Yiming Li, Shanhao Jiang, Dayuan Zhu, Three lycopodium alkaloid N-oxides from Huperzia serrata, Planta Med. 2000, 66: 664
12.Wenyun Gao, Yiming Li, Shanhao Jiang, Dayuan Zhu, A new lycopodium alkaloid from the whole plants of Huperzia serrata, Chin. J. Chem. 2000, 18: 614
13.Wenyun Gao, Yiming Li, Dayuan Zhu, Phenylic glycosides and -lactone glycoside from the sprouts of Crocus sativus,, Planta Med. 1999, 65:
14.Wenyun Gao, Yiming Li, Baode Wang, Dayuan Zhu, Huperzine h, a new lycopodium alkaloid from Huperzia serrata, Chin. Chem. Lett., 1999, 10 (6): 463
15.Wenyun Gao, Yiming Li, Dayuan Zhu, New anthraquinones from the sprouts of Crocus sativus, Acta Bot. Sin., 1999, 41 (5): 531
16.Yiming Li, Shanhao Jiang, Wenyun Gao, Dayuan Zhu, Iridoid glycosides from Scrophularia ningpoensis, Phytochemistry, 1999, 50: 101
17.Yiming Li, Shanhao Jiang, Wenyun Gao, Dayuan Zhu, Phenylpropanoid glycosides from Scrophularia ningpoensis, Phytochemistry, 2000, 54(8): 923
18.Yiming Li, Shanhao Jiang, Wenyun Gao, Dayuan Zhu, Studies of liposoluble constituents from the roots of S. ningpoensis, Acta Pharma. Sin., 1999, 34, 448.
RECENT PATENTS:
1.Wenyun Gao,Heng Li, Jie Tian, Juan Liao, Xuejiao Wang, Hui Wang, Shaoqing Yang, Chemoenzymatic methods to prepare 1-deoxy-D-xylulose, Zhong Guo Fa Ming Zhuan Li, Application Nr.: 71.
2.Wenyun Gao,Heng Li, Jie Tian, Hui Wang, Shaoqing Yang, Methods for screening of the inhibitors of 1-deoxy-D-xylulose 5-phosphate recuctoisomerase form plant extracts, Zhong Guo Fa Ming Zhuan Li, Application Nr.: 53.
3.Wenyun Gao, Jie Tian, Juan Liao, Heng Li, Xuejiao Wang, Shaobo Dai, Chemoenzymatic methods to prepare 1-deoxy-D-xylulose 5-phosphate, Zhong Guo Fa Ming Zhuan Li, Application Nr.: 49.
4.Chao Chen, Wenyun Gao,Tengxiao Zhang,Preparation of phenothiazinium photosensitizer modified magnetic particles for blood viruses Inactivation,Zhong Guo Fa Ming Zhuan Li, Application Nr.: 3.7.
5.Wenyun Gao,Chao Chen,Methods for blood purification, Zhong Guo Fa Ming Zhuan Li, Application Nr.: 5.6.
6.Chao Chen, Wenyun Gao, Jiayi Chen,Methods for the preparation of lyophilized hemoglobin, Zhong Guo Fa Ming Zhuan Li, Application Nr.: 1.4.
Dr. Wen-Yun Gao
Professor of Bioorganic/Organic Chemistry
College of Life Sciences, Northwest University
229 North Taibai Road, Xi’an, 710069, P. R. China
E-mail: gaowenyun@nwu.edu.cn
Tel: 86- ext. 852 (lab), Fax: 86-
RESEARCH INTERESTS:
?Biosynthesis of terpenoids by Mevonolate/MEP pathways
?Preparation of enzymes and enzyme-catalyzed reactions
?Isolation and structure elucidation of bioactive natural products
FUNDS:
?National Scientific Foundation of China
?Department of Education, China
RESEARCH, TEACHING AND WORK EXPERIENCE
July 2006?Present, Professor of bioorganic chemistry, College of Life Sciences, Northwest University
Nov. 2003?June 2006, Postdoctoral fellow, University of Utah, Bioorganic Chemistry
Mentor: Prof. C. Dale Poulter
Sept. 2000?Oct. 2003, Alexander von Humboldt research fellow and guest scientist,
Biocenter, Martin-Luther University, Germany, Bioorganic Chemistry
Mentor: Prof. Dr. M. H. Zenk
July, 1998?June, 2000, Research associate, Shanghai Institute of Organic Chemistry (SIOC), Chinese Academy of Sciences, Organic synthesis
Mentor: Prof. Guoqiang Lin
June, 1992?August, 1995, Lecturer, Lanzhou University, Analytical Chemistry
EDUCATION
?PhD, Organic Chemistry, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 1998
?BS/MS, Analytical Chemistry, Lanzhou University, 1985-1992
AWARDS/HONORS
?Alexander von Humboldt Scholarship from 2001 to 2003.
SELECTED PUBLICATIONS:
1.Zhou, Y.F., Cui, Z., Li, H., Tian, J., Gao, Wenyun, Optimized enzymatic preparation of 1-deoxy-D-xylulose 5-phosphate. Bioorg. Chem. 2010. 38, 120-123.
2.Li, H., Tian, J., Wang, H., Yang, S.Q., Gao, Wenyun, An improved preparation of D-glyceraldehyde 3-phosphate and its use in the synthesis of 1-deoxy-D-xylulose 5-phosphate. Helv. Chim. Acta 2010, 93, 1745-1750.
3.Li, H., Yang, S.Q., Wang, H.,Tian, J., Gao, Wenyun, Biosynthesis of lamalbid in L. barbatum. Phytochemistry, 2010, 71, 1690-1694.
4.Gao, Wenyun, Preparation of isotope labeled/unlabeled 1-deoxy-D-xylulose 5-phosphate and 2-methyl-D-erythreitol 4-phosphate, You Ji Hua Xue, 2010, 30, 23-37.
5.Wenyun Gao, Yimin Li, Shanhao Jiang, Dayuan Zhu, Two lycopodium alkaloid N-oxides from Huperzia serrata, Helvetica Chimica Acta, 2008, 91, 1031-1035.
6.Wenyun Gao, R. Loeser, M. Raschke, M. A. Dessoy, M. Fulhorst, H. Alpermann, L. A. Wessjohann, M. H. Zenk, (E)-4-Hydroxy-3-methylbut-2-enyl diphosphate: an intermediate in the formation of terpenoids in plant chromoplasts. Angew. Chem., Intl. Ed., 2002, 41, 2604-2607.
7.U.-I. Flügge, Wenyun Gao, Transport of Isoprenoid Intermediates Across Chloroplast Envelope Membranes, Plant Biol (Stuttg) 2005, 7, 91-97. (SCI)
8.Gao, Wenyun; M. Raschke; H. Alpermann; M. H. Zenk. A facile enzymatic synthesis of isotopically labeled 2-methyl-D-erythritol 2, 4-cyclo-diphosphate by spinach chloroplast stroma. Helv. Chim. Acta, 2003, 86, 3568-3577.
9.J. E. Page, G. Hause, M. Raschke, Wenyun Gao, J. Schmidt, M. H. Zenk, T. M. Kutchan, Functional Analysis of the Final Steps of the 1-Deoxy-D-xylulose 5-phosphate (DXP) Pathway to Isoprenoids in Plants Using Virus-Induced Gene Silencing, Plant Physiology, 2004, 134, 1401–1413.
10.Brandt, W.; Dessoy, M.A.; Fulhorst, M.; Gao, Wenyun; Zenk, M.H.; Wessjohann, L. A. A proposed mechanism for the reductive ring opening of the cyclodiphosphate mecpp, a crucial transformation in the new DXP/MEP pathway to isoprenoids based on modeling studies and feeding experiments. ChemBioChem, 2004, 311-323.
11.Wenyun Gao, Yiming Li, Shanhao Jiang, Dayuan Zhu, Three lycopodium alkaloid N-oxides from Huperzia serrata, Planta Med. 2000, 66: 664
12.Wenyun Gao, Yiming Li, Shanhao Jiang, Dayuan Zhu, A new lycopodium alkaloid from the whole plants of Huperzia serrata, Chin. J. Chem. 2000, 18: 614
13.Wenyun Gao, Yiming Li, Dayuan Zhu, Phenylic glycosides and -lactone glycoside from the sprouts of Crocus sativus,, Planta Med. 1999, 65:
14.Wenyun Gao, Yiming Li, Baode Wang, Dayuan Zhu, Huperzine h, a new lycopodium alkaloid from Huperzia serrata, Chin. Chem. Lett., 1999, 10 (6): 463
15.Wenyun Gao, Yiming Li, Dayuan Zhu, New anthraquinones from the sprouts of Crocus sativus, Acta Bot. Sin., 1999, 41 (5): 531
16.Yiming Li, Shanhao Jiang, Wenyun Gao, Dayuan Zhu, Iridoid glycosides from Scrophularia ningpoensis, Phytochemistry, 1999, 50: 101
17.Yiming Li, Shanhao Jiang, Wenyun Gao, Dayuan Zhu, Phenylpropanoid glycosides from Scrophularia ningpoensis, Phytochemistry, 2000, 54(8): 923
18.Yiming Li, Shanhao Jiang, Wenyun Gao, Dayuan Zhu, Studies of liposoluble constituents from the roots of S. ningpoensis, Acta Pharma. Sin., 1999, 34, 448.
RECENT PATENTS:
1.Wenyun Gao,Heng Li, Jie Tian, Juan Liao, Xuejiao Wang, Hui Wang, Shaoqing Yang, Chemoenzymatic methods to prepare 1-deoxy-D-xylulose, Zhong Guo Fa Ming Zhuan Li, Application Nr.: 71.
2.Wenyun Gao,Heng Li, Jie Tian, Hui Wang, Shaoqing Yang, Methods for screening of the inhibitors of 1-deoxy-D-xylulose 5-phosphate recuctoisomerase form plant extracts, Zhong Guo Fa Ming Zhuan Li, Application Nr.: 53.
3.Wenyun Gao, Jie Tian, Juan Liao, Heng Li, Xuejiao Wang, Shaobo Dai, Chemoenzymatic methods to prepare 1-deoxy-D-xylulose 5-phosphate, Zhong Guo Fa Ming Zhuan Li, Application Nr.: 49.
4.Chao Chen, Wenyun Gao,Tengxiao Zhang,Preparation of phenothiazinium photosensitizer modified magnetic particles for blood viruses Inactivation,Zhong Guo Fa Ming Zhuan Li, Application Nr.: 3.7.
5.Wenyun Gao,Chao Chen,Methods for blood purification, Zhong Guo Fa Ming Zhuan Li, Application Nr.: 5.6.
6.Chao Chen, Wenyun Gao, Jiayi Chen,Methods for the preparation of lyophilized hemoglobin, Zhong Guo Fa Ming Zhuan Li, Application Nr.: 1.4.