Wang, Wenjing; Sheng, Yanqing
发表期刊WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
ISSN0959-3993
2021-01
卷号37期号:1页码:3
关键词AmpicillinCarotenoid biosynthesisChlorella sorokinianaFatty acid biosynthesisPorphyrin and chlorophyll metabolism
DOI10.1007/s11274-020-02985-1
英文摘要Ampicillin sodium salt (AMP) is commonly and effectively used to prevent bacterial infection in algal culture, but the response of algal strains to AMP has not been investigated. In this study, Chlorella sorokiniana was selected to evaluate the influence of AMP on algae. AMP enhanced the contents of chlorophyll and two fatty acids, myristic acid (C22:1N9) and tetracosanoic acid (C6:0), but inhibited the growth, carotenoid production, and contents of 16 fatty acids in C. sorokiniana. A global transcriptome analysis from experimental data identified 3 825 upregulated and 1 432 downregulated differentially expressed genes (DEGs) in C. sorokiniana. The upregulated DEGs, such as hemB/alaD, mmaB/pduO, cox15/ctaA, fxN, cpoX/hemF, and earS/gltX, were enriched in the porphyrin and chlorophyll metabolism pathways, whereas the downregulated DEGs, including lcyB (crtL1), crtY (lcyE, crtL2), lut1 (CYP97C1), z-isO, crtZ and crtisO (crtH), were enriched in the carotenoid biosynthesis pathway, and the downregulated DEGs, abH, fadD, fabF, acsL, fabG, and accD were enriched in the fatty acid biosynthesis pathway. Thus, the use of AMP to obtain an axenic strain revealed that AMP might affect the regulatory dynamics and the results of the metabolic process in C. sorokiniana. The data obtained in the study provide foundational information for algal purification and aseptic processing.Graphic abstract
资助机构National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31600370, 41373100]; Science and Technology Plan of Yantai City [2018ZHGY080, 2018ZHGY083]; Regional Key Project of STS of the Chinese Academy of Sciences [KFJ-STS-QYZX-057]
收录类别SCI
语种英语
关键词[WOS]RNA-SEQ DATA; AXENIC CULTURES; GENE-TRANSFER; BACTERIA; CAROTENOIDS; ALGAE; ANTIBIOTICS; CULTIVATION; EXPRESSION; EVOLUTION
研究领域[WOS]Biotechnology & Applied Microbiology
WOS记录号WOS:000606495400002
引用统计
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/30506
专题中科院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心
作者单位Chinese Acad Sci, Res Ctr Coastal Environm Engn Technol Shandong Pr, Yantai Inst Coastal Zone Res, Yantai, Shandong, Peoples R China
推荐引用方式
GB/T 7714Wang, Wenjing,Sheng, Yanqing. Ampicillin used in aseptic processing influences the production of pigments and fatty acids in Chlorella sorokiniana[J]. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,2021,37(1):3.
APAWang, Wenjing,&Sheng, Yanqing.(2021).Ampicillin used in aseptic processing influences the production of pigments and fatty acids in Chlorella sorokiniana.WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,37(1),3.
MLAWang, Wenjing,et al."Ampicillin used in aseptic processing influences the production of pigments and fatty acids in Chlorella sorokiniana".WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 37.1(2021):3.
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