Han, Yijing1,2,3; Hu, Gege1; Chen, Yuying4; Chen, Lizhu4; Yu, Daode5; Zhang, Qianqian1,2
; Yang, Dinglong1,2发表期刊FRONTIERS IN MARINE SCIENCE
2021-07-16
卷号8页码:10
关键词Ruditapes philippinarumdefensinextracellular trapsantimicrobial effectmembrane permeabilization
DOI10.3389/fmars.2021.690879
通讯作者Yu, Daode(yudaode@shandong.cn); Yang, Dinglong(dlyang@yic.ac.cn)
英文摘要Although defensins have been isolated from a variety of metazoan, their role in cellular immunity has not been answered. In the study, we found that the hemocytes of the Manila clams Ruditapes philippinarum release defensin (designated as Rpdef3) in response to Vibrio parahaemolyticus challenge. The antimicrobial Rpdef3 was proved to be involved in the extracellular traps (ETs) that hemocytes released in response to Vibrio challenge. Scanning electron microscopy observation proved the patterns how ETs eliminate invading bacteria. Furthermore, Rpdef3 involved in ETs had broad-spectrum antimicrobial effect on both Gram-negative bacteria and Gram-positive bacteria. ELISA assay revealed that Rpdef3 could bind lipopolysaccharides and peptidoglycan in a dose-dependent manner. As concerned to the antibacterial mechanisms, Rpdef3 can cause bacterial membrane permeabilization, leading to cell death. As a result, Rpdef3 might contribute to the trap and the elimination of invading Vibrio in clam ETs. Taken together, our study suggest that the formation of ETs is a defense mechanism triggered by bacterial stimulation, coupled with antibacterial defensin.
资助机构National Natural Science Foundation of China; Natural Science Foundation of Shandong Province; Yantai Science and Technology Development Project; Youth Innovation Promotion Association of CAS
收录类别SCI
语种英语
关键词[WOS]NEUTROPHIL EXTRACELLULAR TRAPS; ESCHERICHIA-COLI; ANTIBACTERIAL ACTIVITIES; MEMBRANE; PEPTIDES; SHRIMP; MECHANISMS; HEMOCYTES; JAPONICUS; MOTILITY
研究领域[WOS]Environmental Sciences & Ecology; Marine & Freshwater Biology
WOS记录号WOS:000680690300001
引用统计
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/29528
专题支撑部门_中国科学院牟平海岸带环境综合试验站
海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心
通讯作者Yu, Daode; Yang, Dinglong作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai, Peoples R China
2.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao, Peoples R China
3.Univ Chinese Acad Sci, Beijing, Peoples R China
4.Shandong Marine Resource & Environm Res Inst, Yantai, Peoples R China
5.Marine Sci Res Inst Shandong Prov, Qingdao, Peoples R China
推荐引用方式
GB/T 7714Han, Yijing,Hu, Gege,Chen, Yuying,et al. Antimicrobial Defensin and DNA Traps in Manila Clam Ruditapes philippinarum: Implications for Their Roles in Immune Responses[J]. FRONTIERS IN MARINE SCIENCE,2021,8:10.
APAHan, Yijing.,Hu, Gege.,Chen, Yuying.,Chen, Lizhu.,Yu, Daode.,...&Yang, Dinglong.(2021).Antimicrobial Defensin and DNA Traps in Manila Clam Ruditapes philippinarum: Implications for Their Roles in Immune Responses.FRONTIERS IN MARINE SCIENCE,8,10.
MLAHan, Yijing,et al."Antimicrobial Defensin and DNA Traps in Manila Clam Ruditapes philippinarum: Implications for Their Roles in Immune Responses".FRONTIERS IN MARINE SCIENCE 8(2021):10.
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