Han, Yijing2,3; Chen, Lizhu4; Yang, Dinglong1,2
发表期刊JOURNAL OF INVERTEBRATE PATHOLOGY
ISSN0022-2011
2021-07-01
卷号183页码:6
关键词Ruditapes philippinarumUbiquitinAntibacterial activityBacteriostatic activity
DOI10.1016/j.jip.2021.107602
通讯作者Yang, Dinglong(dlyang@yic.ac.cn)
英文摘要In the present study, a ubiquitin (designated as RpUbi) was identified and characterized from clam Ruditapes philippinarum. Phylogenetic analysis strongly suggested that RpUbi was a member of the ubiquitin family. In nonstimulated clams, RpUbi transcripts were constitutively expressed in all examined tissues, especially in the gills and hemocytes. After Vibrio anguillarum challenge, expression of RpUbi mRNA in hemocytes was significantly upregulated. Recombinant RpUbi (rRpUbi) showed high antibacterial activity against Gram-positive and Gramnegative bacteria. Notably, membrane integrity and electrochemical assay indicated that rRpUbi could invade the inner layer. Moreover, DNA migration could be inhibited by rRpUbi in a concentration-dependent manner. In general, our results suggested that RpUbi played an important role in host defense against invading bacteria, perhaps through a DNA-binding process.
资助机构Strategic Priority Research Program of the Chinese Academy of Sciences; National Natural Science Foundation of China; Natural Science Foundation of Shandong Province; Yantai Science and Technology Development Project; Youth Innovation Promotion Association of CAS
收录类别SCI
语种英语
关键词[WOS]BROWN RING DISEASE; MANILA CLAM; MOLECULAR CHARACTERIZATION; CONCERTED EVOLUTION; POLYUBIQUITIN GENE; EXPRESSION; PROTEIN; IDENTIFICATION; PURIFICATION; MEMBRANES
研究领域[WOS]Zoology
WOS记录号WOS:000670119900003
引用统计
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/29453
专题海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心
通讯作者Yang, Dinglong作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai 264003, Peoples R China
2.Chinese Acad Sci, Res & Dev Ctr Efficient Utilizat Coastal Bioresou, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Shandong Marine Resource & Environm Res Inst, Yantai 264006, Peoples R China
推荐引用方式
GB/T 7714Han, Yijing,Chen, Lizhu,Yang, Dinglong. Antibacterial activity and mechanism of a type-I ubiquitin from the clam Ruditapes philippinarum[J]. JOURNAL OF INVERTEBRATE PATHOLOGY,2021,183:6.
APAHan, Yijing,Chen, Lizhu,&Yang, Dinglong.(2021).Antibacterial activity and mechanism of a type-I ubiquitin from the clam Ruditapes philippinarum.JOURNAL OF INVERTEBRATE PATHOLOGY,183,6.
MLAHan, Yijing,et al."Antibacterial activity and mechanism of a type-I ubiquitin from the clam Ruditapes philippinarum".JOURNAL OF INVERTEBRATE PATHOLOGY 183(2021):6.
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