Han, Yijing1,2,3; Zhang, Qianqian1,2
; Chen, Lizhu4; Yang, Dinglong1,2; Zhao, Jianmin1,2,5发表期刊DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN0145-305X
2021-08-01
卷号121页码:10
关键词Ruditapes philippinarumExtracellular trapsReactive oxygen speciesMitochondriaAntibacterial activity
DOI10.1016/j.dci.2021.104094
通讯作者Yang, Dinglong(dlyang@yic.ac.cn); Zhao, Jianmin(jmzhao@yic.ac.cn)
英文摘要The formation of extracellular traps (ETs) is an important innate immune mechanism that serves to combat different invading pathogens. In this study, zymosan significantly induced the formation of ETs in the hemocytes of Ruditapes philippinarum, and this effect was accompanied by translocation of the mitochondria to the cell surface. Zymosan stimulation clearly induced an increase in intracellular ROS and MPO production and an overexpression of ROS-related genes (PI3K, AKT and HIF). In response to the ROS burst, the mitochondrial membrane potential decreased, and the mitochondrial permeability transition pore opened. Conversely, mitochondrial superoxide inhibitor (Mito-TEMPO) significantly inhibited the formation of ETs, suggesting that mitochondrial ROS were necessary for the formation of ETs. In addition, we found that zymosan-induced ETs showed antibacterial activities against gram-negative and gram-positive bacteria, such as Vibrio anguillarum, Vibrio harveyi, Escherichia coli and Micrococcus luteus. Taken together, these findings elucidated a new antibacterial approach for R. philippinarum and highlighted the role of mitochondria in the formation of zymosaninduced ETs.
资助机构National Natural Science Foundation of China; Natural Science Foundation of Shandong Province; Yantai Science and Technology Development Project; Youth Innovation Promotion Association, Chinese Academy of Sciences
收录类别SCI
语种英语
关键词[WOS]VIBRIO-TAPETIS; MANILA CLAMS; DNA; RELEASE; MYELOPEROXIDASE; IDENTIFICATION; BACTERIA; CARP
研究领域[WOS]Fisheries; Immunology; Veterinary Sciences; Zoology
WOS记录号WOS:000663315400003
引用统计
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/29434
专题支撑部门_中国科学院牟平海岸带环境综合试验站
海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心
通讯作者Yang, Dinglong; Zhao, Jianmin作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai 264003, Shandong, Peoples R China
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res & Dev Ctr Efficient Utilizat Coastal Bioresou, Yantai 264003, Shandong, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Shandong Marine Resource & Environm Res Inst, Yantai 264006, Shandong, Peoples R China
5.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Shandong, Peoples R China
推荐引用方式
GB/T 7714Han, Yijing,Zhang, Qianqian,Chen, Lizhu,et al. Mitochondria are essential for antibacterial extracellular trap formation mediated by zymosan in hemocytes of Ruditapes philippinarum[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2021,121:10.
APAHan, Yijing,Zhang, Qianqian,Chen, Lizhu,Yang, Dinglong,&Zhao, Jianmin.(2021).Mitochondria are essential for antibacterial extracellular trap formation mediated by zymosan in hemocytes of Ruditapes philippinarum.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,121,10.
MLAHan, Yijing,et al."Mitochondria are essential for antibacterial extracellular trap formation mediated by zymosan in hemocytes of Ruditapes philippinarum".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 121(2021):10.
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