Liu, Guohua1; Liu, Xiaoling1; Liu, Wei2
; Gao, Kangning3; Chen, Xiaoli4; Wang, En-Tao5; Zhao, Zhenjun1; Du, Wenxiao1; Li, Yan1,2发表期刊FRONTIERS IN MICROBIOLOGY
ISSN1664-302X
2021-05-04
卷号12页码:11
关键词rhizobiaBradyrhizobiumdiversityphylogenyVignadistribution
DOI10.3389/fmicb.2021.665839
通讯作者Zhao, Zhenjun(zzjor@sina.com); Du, Wenxiao(duwenxiao2007@163.com); Li, Yan(liyan0709@hotmail.com)
英文摘要Vigna minima is a climbing annual plant widely distributed in barren wilderness, grass land, and shrub bush of China and other countries such as Japan. However, the rhizobia nodulating with this plant has never been systematically studied. In order to reveal the biodiversity of nodulating rhizobia symbiosis with V. minima, a total of 874 rhizobium isolates were obtained from root nodules of the plant spread in 11 sampling sites of Shandong Peninsula, China, and they were designated as 41 haplotypes in the genus Bradyrhizobium based upon recA sequence analyses. By multilocus sequence analysis (MLSA) of five housekeeping genes (dnaK, glnII, gyrB, recA, and rpoB), the 41 strains representing different recA haplotypes were classified into nine defined species and nine novel genospecies. Bradyrhizobium elkanii, Bradyrhizobium ferriligni, and Bradyrhizobium pachyrhizi were the predominant and universally distributed groups. The phylogeny of symbiotic genes of nodC and nifH showed similar topology and phylogenetic relationships, in which all the representative strains were classified into two clades grouped with strains nodulating with Vigna spp., demonstrating that Vigna spp. shared common nodulating groups in the natural environment. All the representative strains formed nodules with V. minima in a nodulation test performed in green house conditions. The correlation between V. minima nodulating rhizobia and soil characteristics analyzed by CANOCO indicates that available nitrogen, total nitrogen, and organic carbon in the soil samples were the main factors affecting the distribution of rhizobia isolated in this study. This study systematically uncovered the biodiversity and distribution characteristics of V. minima nodulating rhizobia for the first time, which provided novel information for the formation of the corresponding rhizobium community.
资助机构National Natural Science Foundation of China; Joint Fund of Jilin Province and Chinese Academy of Sciences for High-Tech Industrialization; Key Research and Development Program of Hebei Province; IPN, Mexico
收录类别SCI
语种英语
关键词[WOS]MULTILOCUS SEQUENCE-ANALYSIS; SP NOV.; SUBTROPICAL REGION; GENETIC DIVERSITY; ROOT-NODULES; UNGUICULATA L.; SALT TOLERANCE; BRADYRHIZOBIUM; RADIATA; BACTERIUM
研究领域[WOS]Microbiology
WOS记录号WOS:000651404100001
引用统计被引频次：1[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/29328
专题海岸带生物学与生物资源利用重点实验室
海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
通讯作者Zhao, Zhenjun; Du, Wenxiao; Li, Yan作者单位1.Yantai Univ, Coll Life Sci, Yantai, Peoples R China
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai, Peoples R China
3.Shijiazhuang Univ, Coll Resources & Environm, Shijiazhuang, Hebei, Peoples R China
4.Fruit Trees Work Stn Penglai, Yantai, Peoples R China
5.Inst Politecn Nacl, Escuela Nacl Ciencias Biol, Dept Microbiol, Mexico City, DF, Mexico
推荐引用方式
GB/T 7714Liu, Guohua,Liu, Xiaoling,Liu, Wei,et al. Biodiversity and Geographic Distribution of Rhizobia Nodulating With Vigna minima[J]. FRONTIERS IN MICROBIOLOGY,2021,12:11.
APALiu, Guohua.,Liu, Xiaoling.,Liu, Wei.,Gao, Kangning.,Chen, Xiaoli.,...&Li, Yan.(2021).Biodiversity and Geographic Distribution of Rhizobia Nodulating With Vigna minima.FRONTIERS IN MICROBIOLOGY,12,11.
MLALiu, Guohua,et al."Biodiversity and Geographic Distribution of Rhizobia Nodulating With Vigna minima".FRONTIERS IN MICROBIOLOGY 12(2021):11.
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