Ge, Baosheng1,2; Wang, Haonan1,2; Li, Jie1,2,3
; Liu, Hengheng1,2; Yin, Yonghao1,2; Zhang, Naili4; Qin, Song3发表期刊MARINE DRUGS
2020-04-01
卷号18期号:4页码:16
关键词collagen hydrogelfish skincharacterizationwound dressing
DOI10.3390/md18040178
通讯作者Ge, Baosheng(gebaosheng@upc.edu.cn); Zhang, Naili(znl-0@163.com); Qin, Song(sqin@yic.ac.cn)
英文摘要Collagen plays an important role in the formation of extracellular matrix (ECM) and development/migration of cells and tissues. Here we report the preparation of collagen and collagen hydrogel from the skin of tilapia and an evaluation of their potential as a wound dressing for the treatment of refractory wounds. The acid-soluble collagen (ASC) and pepsin-soluble collagen (PSC) were extracted and characterized using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), differential scanning calorimetry (DSC), circular dichroism (CD) and Fourier transform infrared spectroscopy (FTIR) analysis. Both ASC and PSC belong to type I collagen and have a complete triple helix structure, but PSC shows lower molecular weight and thermal stability, and has the inherent low antigenicity. Therefore, PSC was selected to prepare biomedical hydrogels using its self-aggregating properties. Rheological characterization showed that the mechanical strength of the hydrogels increased as the PSC content increased. Scanning electron microscope (SEM) analysis indicated that hydrogels could form a regular network structure at a suitable PSC content. Cytotoxicity experiments confirmed that hydrogels with different PSC content showed no significant toxicity to fibroblasts. Skin repair experiments and pathological analysis showed that the collagen hydrogels wound dressing could significantly accelerate the healing of deep second-degree burn wounds and the generation of new skin appendages, which can be used for treatment of various refractory wounds.
资助机构National Natural Science Foundation of China; major basic research plan of Shandong Province; Shandong Province Higher Educational Science and Technology Program; Natural Science Foundation of Shandong Province
收录类别SCI
语种英语
关键词[WOS]PEPSIN-SOLUBLE COLLAGEN; CROSS-LINKING; I COLLAGEN; ACID; FIBROBLASTS; SCAFFOLD; GELATIN; EXTRACTION; BIOCOMPATIBILITY; BIOMATERIALS
研究领域[WOS]Pharmacology & Pharmacy
WOS记录号WOS:000533909300029
引用统计被引频次：14[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/29059
专题海岸带生物学与生物资源利用重点实验室
海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
通讯作者Ge, Baosheng; Zhang, Naili; Qin, Song作者单位1.China Univ Petr East China, State Key Lab Heavy Oil Proc, Qingdao 266580, Peoples R China
2.China Univ Petr East China, Ctr Bioengn & Biotechnol, Qingdao 266580, Peoples R China
3.Chinese Acad Sci, Yantai Inst Coastal Zone, Yantai 264003, Peoples R China
4.Binzhou Med Univ, Sch Basic Med Sci, Dept Human Anat, 346 Guanhai Rd, Laishan 264003, Yantai, Peoples R China
推荐引用方式
GB/T 7714Ge, Baosheng,Wang, Haonan,Li, Jie,et al. Comprehensive Assessment of Nile Tilapia Skin (Oreochromis niloticus) Collagen Hydrogels for Wound Dressings[J]. MARINE DRUGS,2020,18(4):16.
APAGe, Baosheng.,Wang, Haonan.,Li, Jie.,Liu, Hengheng.,Yin, Yonghao.,...&Qin, Song.(2020).Comprehensive Assessment of Nile Tilapia Skin (Oreochromis niloticus) Collagen Hydrogels for Wound Dressings.MARINE DRUGS,18(4),16.
MLAGe, Baosheng,et al."Comprehensive Assessment of Nile Tilapia Skin (Oreochromis niloticus) Collagen Hydrogels for Wound Dressings".MARINE DRUGS 18.4(2020):16.
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