Bo, Yuntai1; Wang, Kang2; Wu, Yuanyuan1; Cao, Haiyang1; Cui, Yulin3
; Wang, Lan4,5发表期刊JOURNAL OF APPLIED PHYCOLOGY
ISSN0921-8971
2020-07-14
页码7
关键词Tisochrysis luteaGenetic transformationChloroplastBiolistic method
DOI10.1007/s10811-020-02159-4
通讯作者Wang, Lan(WL_312@163.com)
英文摘要Tisochrysis luteais a haptophyte microalga commonly used as a commercial feed for juvenile fish and shellfish larvae. Genetic modification is of considerable importance for developing the potential economic value ofT. lutea. However, the genetic transformation system ofT. luteahas not yet been established, which limits functional genomic studies and strain improvement of this important microalgal species. In the current study, a chloroplast transformation vector harboring the phosphinothricin resistance gene (bar) as a selectable marker was established, and two short peptide-encoding genes (ant1 andant2) driven by the endogenouspsbAandrbcLpromoters were cloned in this vector. The recombinant plasmid was transformed using a biolistic method into thetrnI/trnAlocus of the chloroplast genome via homologous recombination. After continuous selection on phosphinothricin, the integration of foreign genes and the expression of specific products in the transformants were detected using polymerase chain reaction (PCR), Southern blotting, and western blot analysis. This is the first report of establishment of a stable transformation system inT. lutea, which is a prerequisite for functional genomics and applied research on this species.
资助机构National Natural Science Foundation of China; Natural Science Foundation of Shandong Province, China; Project of Innovation & Development of Marine Economy
收录类别SCI
语种英语
关键词[WOS]CULTIVATION MODE; CHLAMYDOMONAS; DNA
研究领域[WOS]Biotechnology & Applied Microbiology; Marine & Freshwater Biology
WOS记录号WOS:000548460700001
引用统计被引频次：1[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/28629
专题海岸带生物学与生物资源利用重点实验室
海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
通讯作者Wang, Lan作者单位1.Yantai Marine Environm Monitoring Ctr Stn SOA, Yantai 264006, Peoples R China
2.Shandong Univ Technol, Sch Life Sci, Zibo 255049, Shandong, Peoples R China
3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resource Utilizat, Yantai 264003, Shandong, Peoples R China
4.Shandong Prov Key Lab Marine Ecol & Environm & Di, Qingdao 266000, Peoples R China
5.North China Sea Marine Forecasting Ctr SOA, 27 Yunling Rd, Qingdao 266000, Shandong, Peoples R China
推荐引用方式
GB/T 7714Bo, Yuntai,Wang, Kang,Wu, Yuanyuan,et al. Establishment of a chloroplast transformation system inTisochrysis lutea[J]. JOURNAL OF APPLIED PHYCOLOGY,2020:7.
APABo, Yuntai,Wang, Kang,Wu, Yuanyuan,Cao, Haiyang,Cui, Yulin,&Wang, Lan.(2020).Establishment of a chloroplast transformation system inTisochrysis lutea.JOURNAL OF APPLIED PHYCOLOGY,7.
MLABo, Yuntai,et al."Establishment of a chloroplast transformation system inTisochrysis lutea".JOURNAL OF APPLIED PHYCOLOGY (2020):7.
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