Wei, Qianyu; Mu, Changkao; Wang, Chunlin; Zhao, Jianmin
发表期刊FISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
2018-10
卷号81页码:502-508
关键词Ruditapes philippinarumc-type lysozymeTissue distributionInnate immunity
研究领域Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences
DOI10.1016/j.fsi.2018.07.036
产权排序[Wei, Qianyu; Mu, Changkao; Wang, Chunlin] Ningbo Univ, Minist Educ, Key Lab Appl Marine Biotechnol, Ningbo 315211, Zhejiang, Peoples R China; [Zhao, Jianmin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China; [Wei, Qianyu] Ningbo Univ, Collaborat Innovat Ctr Zhejiang Marine High Effic, Ningbo 315211, Zhejiang, Peoples R China
作者部门海岸带生物资源高效利用研究与发展中心
英文摘要Lysozyme is an antibacterial enzyme that is widely distributed in nature and plays an important role in the prevention of bacterial infections. In this study, a c-type lysozyme (designated as "RpCLYZ") was cloned and characterized from the manila clam, Ruditapes philippinarum. The full-length cDNA was 619 bp with an open reading frame (ORF) of 447 bp, and encoded a polypeptide of 148 amino acids with a calculated molecular mass of 17.0 kDa and an isoelectric point of 4.83. RpCLYZ was found to share high sequence similarity with c-type lysozymes from other invertebrates. The mRNA transcript of RpCLYZ was universally expressed in a wide range of tissues, especially in gills and mantle. Challenge with Vibrio anguillarum, significantly induced mRNA expression of RpCLYZ, which reached a maximum level 48 h after bacterial challenge. Recombinant RpCLYZ (rRpCLYZ) exhibited antibacterial activities against both Gram-positive and Gram-negative bacteria. Additionally, the optimal pH and temperature for rRpCLYZ activity were determined to be 4.5 and 20 degrees C, respectively. These results suggest that RpCLYZ participates in innate immune responses against bacterial invasion.
文章类型Article
资助机构National Natural Science Foundation of China [41476126]; Chinese Academy of Sciences [XDA11020702, XDA11020305, KZZD-EW-14]; Youth Innovation Promotion Association CAS [2016196]; K C Wong Magana Fund in Ningbo University
收录类别SCI
语种英语
关键词[WOS]MYTILUS-EDULIS; CLONING; MUSSEL
研究领域[WOS]Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences
WOS记录号WOS:000443665900060
引用统计被引频次：4[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/24536
专题海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心
海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
作者单位1.Ningbo Univ, Minist Educ, Key Lab Appl Marine Biotechnol, Ningbo 315211, Zhejiang, Peoples R China;
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China;
3.Ningbo Univ, Collaborat Innovat Ctr Zhejiang Marine High Effic, Ningbo 315211, Zhejiang, Peoples R China
推荐引用方式
GB/T 7714Wei, Qianyu,Mu, Changkao,Wang, Chunlin,et al. Molecular characterization, expression, and antibacterial activity of a c-type lysozyme isolated from the manila clam, Ruditapes philippinarum[J]. FISH & SHELLFISH IMMUNOLOGY,2018,81:502-508.
APAWei, Qianyu,Mu, Changkao,Wang, Chunlin,&Zhao, Jianmin.(2018).Molecular characterization, expression, and antibacterial activity of a c-type lysozyme isolated from the manila clam, Ruditapes philippinarum.FISH & SHELLFISH IMMUNOLOGY,81,502-508.
MLAWei, Qianyu,et al."Molecular characterization, expression, and antibacterial activity of a c-type lysozyme isolated from the manila clam, Ruditapes philippinarum".FISH & SHELLFISH IMMUNOLOGY 81(2018):502-508.
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