陈秀兰 电 话：** 传 真： E-Mail：CXL0423@sdu.edu.cn

教育背景

1998.9-2002.7 山东大学微生物技术国家重点实验室 博士

1989.9-1992.7 山东师范大学生物系 硕士

1985.9-1989.7 山东师范大学生物系 本科

工作经历

起止时间 单位名称 专业技术职务

2004.9-至今 山东大学 教授

2002.9-2004.8 山东大学 副教授

1998.9-2002.7 山东大学 在职博士研究生

1992.7-1998.8 山东省农业科学院 助理研究员

主要研究方向

（1）海洋微生物学

（2）海洋微生物酶学

（3）海洋微生物酶生物技术

承担主要课题

1. 2014-2016 M23家族金属蛋白酶Pseudoalterin的制备及其抑制耐药性金黄葡萄球菌的应用评价, 国家海洋863

2. 2013-2016 深海细菌新型M12家族弹性蛋白酶myroilysin 对胶原蛋白的膨胀机制及应用, 国家自然基金

3. 2012-2015 海洋细菌分泌的PL18家族褐藻酸裂解酶的成熟机制及其N端结构域的功能, 国家自然基金

研究成果

1. Li CY, Wei TD, Zhang SH, Chen XL, Gao X, Wang P, Xie BB, Su HN, Qin QL, Zhang XY, Yu J, Zhang HH, Zhou BC, Yang GP, Zhang YZ*. Molecular insight into bacterial cleavage of oceanic dimethylsulfoniopropionate into dimethyl sulfide. Proc Natl Acad Sci U S A. 2014,111:1026-1031.

2. Ran LY, Su HN, Zhou MY, Wang L, Chen XL, Xie BB, Song XY, Shi M, Qin QL, Pang X, Zhou BC, Zhang YZ*, Zhang XY*. Characterization of a Novel Subtilisin-like Protease Myroicolsin from Deep sea bacterium Myroides profundi D25 and Molecular insight into its Collagenolytic Mechanism. The Journal of Biological Chemistry, 2014, 289(9): 6041-6053.

3. Zi-Chao Yu, Dian-Li Zhao, Li-Yuan Ran, Zi-Hao Mi, Zhao-Yu Wu, Xi-Ying Zhang, Hai-Nan Su, Mei Shi, Xiao-Yan Song, Bin-Bin Xie, Qi-Long Qin, Bai-Cheng Zhou, Xiu-Lan Chen*, Yu-Zhong Zhang, Development of a genetic system for the deep-sea psychrophilic bacterium Pseudoalteromonas sp. SM9913. Microbial cell factories, 2014, 13: 13.

4. Ran LY, Su HN, Zhao GY, Gao X, Zhou MY, Wang P, Zhao HL, Xie BB, Zhang XY, Chen XL, Zhou BC, Zhang YZ*. Structural and Mechanistic Insights into Collagen Degradation by a Bacterial Collagenolytic Serine Protease in the Subtilisin Family. Molecular Microbiology, 2013, 90(5): 997-1010.

5. Guo-Yan Zhao, Ming-Yang Zhou, Hui-Lin Zhao, Xiu-Lan Chen*, Bin-Bin Xie, Xi-Ying Zhang, Hai-Lun He, Bai-Cheng Zhou, Yu-Zhong Zhang. Tenderization effect of cold-adapted collagenolytic protease MCP-01 on beef meat at low temperature and its mechanism. Food Chemistry，2012, 134:1738-1344.

6. Dong S, Yang J, Zhang XY, Shi M, Song XY, Chen XL*, Zhang YZ.， Cultivable alginate lyase-excreting bacteria associated with the arctic brown alga laminaria. Mar Drugs. 2012 Nov 6;10(11):2481-91.

7. Wu Ji-Wei, Chen Xiu-Lan*, Extracellular metalloproteases from bacteria. Appl Microbiol Biotechnol. 2011 Oct;92(2):253-62. Epub 2011 Aug 16.

8. Dian-Li Zhao, Zi-Chao Yu, Ping-Yi Li, Zhao-Yu Wu, Xiu-Lan Chen*, Mei Shi, Yong Yu, Bo Chen, Bai-Cheng Zhou and Yu-Zhong Zhang, Characterization of a cryptic plasmid pSM429 and its application for heterologous expression in psychrophilic Pseudoalteromonas, Microbial Cell Factories, 2011, 10:30 doi:10.1186/1475-2859-10-30

9. Han Zheng, Bing Guo, Xiu-Lan Chen*, Sou-Jin Fan*, Yu-Zhong Zhang, Improvement of the bread quality of wheat flour by glycoside hydrolase family 10 xylanases, Applied Microbiology and Biotechnology, 2011 Apr;90(2):509-15. Epub 2011 Jan 20

10. Jian-Wei Li, Sheng Dong, Jie Song, Chun-Bo Li, Xiu-Lan Chen*, Bin-Bin Xie and Yu-Zhong Zhang Purification and Characterization of a Bifunctional Alginate Lyase from Pseudoalteromonas sp. SM0524 Marine. Drugs, 2011, 9, 109-123; doi:10.3390/md**

11. Xiang Gao, Jue Wang, Da-Qi Yu, Fei Bian, Bin-Bin Xie, Xiu-Lan Chen, Bai-Cheng Zhou, Lu-Hua Lai, Zhi-Xin Wang, Jia-Wei Wu*, Yu-Zhong Zhang*, Structural Basis for the Autoprocessing of Zinc Metalloproteases in the Thermolysin Family. Proc Natl Acad Sci U S A. 2010, 107(41):17569-74.

12. Qi-Long Qin, Xi-Ying Zhang, Xu-Min Wang, Gui-Ming Liu, Xiu-Lan Chen, Bin-Bin Xie, Hong-Yue Dang, Bai-Cheng Zhou, Jun Yu and Zhang Yu-Zhong*, The complete genome of Zunongwangia profunda SM-A87 reveals its adaptation to the deep-sea environment and ecological role in sedimentary organic nitrogen degradation, BMC Genomics, 2010, 11:247 doi:10.1186/1471-2164-11-247

13. Yu-Kai Wang, Guo-Yan Zhao (co-first author), Yang Li, Xiu-Lan Chen, Bin-Bin Xie, Hai-Nan Su, Yao-Hui Lv, Hai-Lun He, Hong Liu, Jun Hu, Bai-Cheng Zhou, Zhang Yu-Zhong*, Mechanistic insight into the function of the C-terminal PKD domain of the collagenolytic serine protease deseasin MCP-01 from deep-sea Pseudoalteromonas sp. SM9913: Binding of the PKD domain to collagen results in collagen swelling but does not unwind the collagen triple helix, The Journal of Biological Chemistry, 2010, 285(19):14285-91

14. Yu-Kai Wang , Hai-Lun He, Guo-Fan Wang, Hao Wu 1, Bai-Cheng Zhou, Xiu-Lan Chen*, Yu-Zhong Zhang, Oyster (Crassostrea gigas) Hydrolysates Produced on a Plant Scale Have Antitumor Activity and Immunostimulating Effects in BALB/c Mice, Maine Drugs, 2010, 8:255-268

15. Bin-Bin Xie, Fei Bian, Xiu-Lan Chen, Hai-Lun He, Jun Guo, Xiang Gao, Yin-Xin Zeng, Bo Chen, Bai-Cheng Zhou, and Yu-Zhong Zhang*, Cold adaptation of zinc metalloproteases in the thermolysin family from deep-sea and Arctic sea-ice bacteria revealed by catalytic and structural properties and molecular dynamics: New insights into relationship between conformational flexibility and hydrogen-bonding, Journal of Biological Chemistry，2009, 284: 9257-9269.

16. Hai-Lun He, Xiu-Lan Chen(co-first author), Xi-Ying Zhang, Cai-Yun Sun, Bai-Cheng Zou, Yu-Zhong Zhang*, Novel Use for Osmolyte Trimethylamine N-oxide: Retaining the Psychrophilic Characters of Cold-adapted Protease Deseasin MCP-01 and simultaneously Improving Its Thermostability, Marine Biotechnology, 2009, 11: 710-716.

17. Bing Guo, Xiu-Lan Chen *, Cai-Yun Sun, Bai-Cheng Zhou and Yu-Zhong Zhang, Gene cloning, expression and characterization of a new cold-active and salt-tolerant endo-β-1,4-xylanase from marine Glaciecola mesophila KMM 241, Applied Microbiology and Biotechnology, 2009, 84(6): 1107-15.

18. Bing-Qiang Yan, Xiu-Lan Chen(co-first author), Xiao-Yan Hou, Hailun He, Bai-Cheng Zhou, Yu-Zhong Zhang *, Molecular Analysis of the Gene Encoding a Cold-Adapted Halophilic Subtilase from Deep-sea Psychrotolerant Bacterium Pseudoalteromonas sp. SM9913: Cloning, Expression, Characterization and Function Analysis of the C-terminal PPC Domains, Extremophiles, 2009 , 13(4): 725-33.

19. Xiu-Lan Chen, Bin-Bin Xie, Fei Bian, Guo-Yan Zhao, Hui-Lin Zhao, Hai-Lun He, Bai-Cheng Zhou, Yu-Zhong Zhang*, Myroilysin, a Novel Bacterial M12 Metalloprotease with Elastinolytic Activity and Synergistic Role with Collagenase in Collagen Hydrolysis by Swelling Collagen: Implication for Its Ecological Function in Biodegradation of Deep-Sea High-Molecular-Weight Organic Nitrogen, Applied and Environmental Microbiology, 2009, 75, 1838-44

20. Ming-Yang Zhou, Xiu-Lan Chen(co-first author), Hui-Lin Zhao, Hong-Yue Dang, Xi-Wu Luan, Xi-Ying Zhang, Hai-Lun He, Bai-Cheng Zhou, Yu-Zhong Zhang*, Diversity of both the Cultivable Protease-producing Bacteria and Their Extracellular Proteases in the Sediments of the South China Sea, Microbial Ecology, 2009, 58(3): 582-90.

21. Min Zhang, Xiu-Lan Chen(co-first author), Zhi-Hua Zhang, Cai-Yun Sun, Lei-Lei Chen, Hai-Lun He, Bai-Cheng Zhou, Yu-Zhong Zhang*, Characterization of Endo-beta-mannanase MAN5 from Bacillus sp. MSJ-5 and Its Application in Preparation of Oligosaccharides from Konjac Flour, Applied and Microbiology Biotechnology, 2009, 83(5): 865-73.

22. Guo-Yan Zhao, Xiu-Lan Chen (co-first author), Hui-Lin Zhao, Bin-Bin Xie, Bai-Cheng Zhou, Yu-Zhong Zhang*, Hydrolysis of insoluble collagen by deseasin MCP-01 from deep-sea Pseudoalteromonas sp. SM9913: Collagenolytic characters, collagen-binding ability of C-terminal PKD domain and Implication for its novel role in deep-sea sedimentary particulate organic nitrogen degradation, Journal of Biological Chemistry，2008, 283 (52): 36100-36107.

23. Xiu-Lan Chen, Bin-Bin Xie, Jing-Tao Lu, Hai-Lun He, Yu-Zhong Zhang*，A novel type of subtilase from the psychrotolerant bacterium Pseudoalteromonas sp. SM9913: catalytic and structural properties of deseasin MCP-01. Microbiology-SGM，2007, 153: 2116-2125.

24. Xiu-Lan Chen, Yuzhong Zhang*, Jing-Tao Lu, Bin-Bin Xie, Cai-Yun Sun, Bing Guo，Autolysis of a novel multidomain subtilase—Cold-adapted deseasin MCP-01 is pH-dependent and the surface loops in its catalytic domain, the linker, and the P-proprotein domain are susceptible to proteolytic attack, Biochem. Biophys. Res. Commun., 2007, 358(3): 704-709.