岑卫^1,2, 胡梦珠^1,2, Rinkevich Baruch³, 胡国斌^1,2
1.中国海洋大学, 海洋生命学院, 山东 青岛 266003;2.中国海洋大学, 海洋生物多样性与进化研究所, 山东 青岛 266003;3.以色列国家海洋研究所, 海洋和湖沼学研究所, 海法 31080 以色列

摘要:
细胞系的构建在生物学领域具有重要意义，与其他动物（如昆虫）不同，经过数十年的发展，海洋无脊椎动物细胞研究仍停留在原代培养水平，没有建立起克隆化的永生细胞系。本文主要介绍了近年来海洋无脊椎动物细胞培养的研究进展，还介绍了一个基本原理，即海洋无脊椎动物永生细胞系的建立，可以通过捕获细胞的干性而实现。捕获细胞干性的第一条途径是利用转录因子等直接由体细胞制备海洋动物的诱导多能干细胞（iPS细胞）。第二条途径是获取海洋动物的成体干细胞（ASC）。这些细胞在维持机体活性、再生、无性克隆、体内增殖等方面起重要作用。当培养条件正确时，海洋无脊椎动物iPS细胞和ASC将会保持其原始状态，利用干细胞自我复制的固有能力、不分化地进行增殖而形成永生细胞系。
关键词:海洋无脊椎动物细胞培养iPS细胞干细胞永生细胞系
DOI：10.11759/hykx20200613001
分类号:Q178.53;Q813.11
基金项目:山东省自然科学基金（ZR2019MC051），中央高校基本科研业务费（201822025）

Establishment of cell lines from marine invertebrates by in-ducing cellular stemness
CEN Wei^1,2, HU Meng-zhu^1,2, RINKEVICH Baruch³, HU Guo-bin^1,2
1.College of Marine Life Science, Ocean University of China, Qingdao 266003, China;2.Institute of Evolu-tion & Marine Biodiversity, Ocean University of China, Qingdao 266003, China;3.Oceanographic and Limnological Research, National Institute of Oceanography of Israel, Tel-Shikmona, P. O. Box 8030, Haifa 31080, Israel
Abstract:
Establishment of cell lines is of great significance in the field of biology. Contrary to the field of insect research, in which development of various cell lines has become routine, the marine invertebrate cell culture research has remained at the primary culture stage. Not a single marine invertebrate immortal cell line has been established yet even after decades of research. This paper discusses the research progress in the field of marine invertebrate cell culture in the recent years. It also discusses the basic principle behind the development of immortal cell lines from marine invertebrates through induction of cellular stemness. At first, induced pluripotent stem (iPS) cells of marine animals must be prepared. The technology used for developing iPS cells of mammals and other vertebrates is quite developed and involves dedifferentiation of somatic cells into iPS cells using transcription factors. Another method that can be used for developing iPS cells involves the use of adult stem cells (ASCs) from marine animals. There are a large number of ASCs in marine invertebrates; the ASCs play an important role in many processes, such as organism development and reproduction. Under certain culture conditions, iPS cells and ASCs of marine invertebrates can form immortal cell lines using characteristics of self-generated stem cells.
Key words:marine invertebratescell culturesiPS cellsstem cellsimmortal cell line





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