徐利婷^1,2,3,4, 马岩^1,3,4, 张一梦^1,2,3,4, 管方^1,3,4, 翟晓凡^1,3,4, 董续成^1,2,3,4, 段继周^1,3,4, 侯保荣^1,3,4
1.中国科学院海洋研究所, 山东 青岛 266071;2.中国科学院大学, 北京 100049;3.青岛海洋科学与技术国家试点实验室, 山东 青岛 266237;4.中国科学院海洋大科学研究中心, 山东 青岛 266071

摘要:
本研究选用一株野生型Pseudomonas sp.为试验菌株，选用X70管线钢作为代表性金属材料，通过细菌生长曲线测定、腐蚀失重测量、扫描电镜（SEM）观察等方法，初步研究在杀菌剂四羟甲基硫酸磷（THPS）耐受条件下Pseudomonas sp.对X70管线钢腐蚀行为的影响。结果表明，75 μL/L的THPS浓度在Pseudomonas sp.的耐受范围内，而且能够轻微促进X70试片表面的腐蚀；不添加THPS的含菌体系中Pseudomonas sp.能抑制X70试片表面的腐蚀；而在添加了75 μL/L THPS的含菌体系中，Pseudomonas sp.显著促进X70试片的腐蚀，并且试片表面附着的细菌数量相比于无杀菌剂的对照体系有所增加。因此，该研究表明在特定浓度范围内，与没有添加杀菌剂的含菌体系相比，杀菌剂THPS使Pseudomonas sp.从抑制腐蚀改变为促进腐蚀。
关键词:假单胞菌THPS耐药性腐蚀生物膜
DOI：10.11759/hykx20200313001
分类号:TG171
基金项目:中国科学院战略先导专项（XDA13040403），国家自然科学青年基金（41806090，41806163），山东省重点研发计划（2018GHY115029）

Effect of Pseudomonas sp. on the corrosive behavior of metal materials under biocide resistance
XU Li-ting^1,2,3,4, MA Yan^1,3,4, ZHANG Yi-meng^1,2,3,4, GUAN Fang^1,3,4, ZHAI Xiao-fan^1,3,4, DONG Xu-cheng^1,2,3,4, DUAN Ji-zhou^1,3,4, HOU Bao-rong^1,3,4
1.Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;2.University of Chinese Academy of Sciences, Beijing 100049, China;3.Pilot National Laboratory for Marine Science and Technology(Qingdao), Qingdao 266237, China;4.Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China
Abstract:
In this study, we selected a wild-type Pseudomonas sp. as the test strain and X70 pipeline steel as the representative metal material. The purpose of this study is to examine the effect of Pseudomonas sp. on the corrosive behavior of X70 steel under the addition of tetrakis (hydroxymethyl) phosphonium sulfate (THPS). We also assessed bacterial growth curve and measured the corrosion weight loss. Additionally, we performed morphological analyses by using scanning electron microscopy. The results showed that the corrosion rate of X70 test coupons was slightly promoted when the concentration of THPS was 75 μL/L, which was within the tolerance concentration scope of Pseudomonas sp. Similarly, Pseudomonas sp. in an environment without THPS inhibited the corrosive surface of X70 test coupons. However, Pseudomonas sp. significantly promoted the corrosion of X70 coupons in the bacterial THPS system. Meanwhile, as compared with the control group without THPS, there was an increase in the number of bacteria attached on the surface of the X70 coupons. In conclusion, this study revealed that the biocide THPS could change Pseudomonas sp. from inhibiting corrosion to promoting corrosion in a specific concentration range, as compared with the bacteria-containing system without biocide.
Key words:Pseudomonas sp.THPSbiocide resistancecorrosionbiofilm





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