王瑞¹, 黄雨辰¹, 熊德东¹, 黄亚¹, 田甜¹, 孟彬¹, 梁健^1,2
1.天津农学院 水产学院, 天津市水产生态及养殖重点实验室, 天津 300384;2.天津市海洋牧场技术工程中心, 天津 300457

摘要:
采用静态急性毒性实验研究了重金属Pb²⁺对青蛤（Cyclina sinensis）的生物急性毒性效应，测定了在96 h Pb²⁺半致死浓度的1/10（TC组）和1/100（SC组）两个浓度胁迫下，血淋巴液中酸性磷酸酶（ACP）、碱性磷酸酶（AKP）和溶菌酶（LSZ）活性的变化。结果显示：Pb²⁺的96 h LC₅₀为7.938 mg/L；SC组ACP活性表现为诱导-抑制趋势，除4 d外均与对照组差异显著（P<0.05），TC组为抑制趋势，为3个组中的最低，与对照组差异显著（P<0.05）；实验组SC组和TC组的AKP、LSZ活性均表现为前期为诱导中后期受抑制的趋势，与对照组差异显著（P<0.05），且TC组活性始终低于SC组，表现出Pb²⁺的胁迫浓度越高酶活性受到的抑制作用越大。以上结果表明，重金属Pb²⁺对青蛤的毒性级别为高毒级，能造成青蛤免疫相关酶的活性受到抑制，影响青蛤的免疫能力，而且这种抑制作用随着环境中Pb²⁺浓度增加而增加。
关键词:青蛤(Cyclina sinensis)Pb²⁺急性毒性免疫相关酶
DOI：10.11759/hykx20190714002
分类号:S917.4
基金项目:天津市大学生创新创业训练计划项目（201810061254）；天津市农业科技成果转化与推广项目（201903010）；国家贝类产业技术体系项目（CARS-49）；国家重点研发计划"蓝色粮仓科技创新"项目（2018YFD0901404）；天津市种业科技重大专项（17ZXZYNC00020）；天津市水产产业技术体系创新团队（ITTFRS2017013）

Acute Pb²⁺ toxicity in Cyclina sinensis and its effect on immune-related enzyme activity in hemolymph
WANG Rui¹, HUANG Yu-chen¹, XIONG De-dong¹, HUANG Ya¹, TIAN Tian¹, MENG Bin¹, LIANG Jian^1,2
1.Tianjin Key Laboratory of Aqua-ecology and Aquaculture, Department of Fishery Sciences, Tianjin Agricultural College, Tianjin 300384, China;2.Tianjin Marine Ranching Technical Engineering Center, Tianjin 300457, China
Abstract:
Acute lead toxicity has emerged as a serious environmental and health concern in China. The present study aimed to evaluate the effects of acute Pb²⁺ toxicity on Cyclina sinensis using static acute toxicity test. Effects of Pb²⁺ on the activity of three immune-related enzymes-acid phosphatase (ACP), alkaline phosphatase (AKP), and lysozyme (LSZ)-present in hemolymph were studied under stress concentration of 1/10 (TC group) and 1/100 (SC group) of LC₅₀ at 96 h. The results of the study indicated that LC₅₀ of Pb²⁺ was 7.938 mg/L at 96 h post exposure. The activity of ACP in the SC group showed an induction-inhibition trend that was significantly different from the control group, except for 4 days (P<0.05). The inhibition trend for ACP in the TC group was significantly different from the control group and was the lowest among the three groups (P<0.05). For both the SC and TC groups, the activities of AKP and LSZ were significantly induced in early stage, followed by inhibition in middle and late stages of lead exposure. This trend was significantly different from that of the control group (P<0.05), and the activity reported in the TC group was lower than that in the SC group at all stages. The results of this study indicated that high Pb²⁺ toxicity in C. sinensis was accompanied by the inhibition of immune-related enzymes, thereby affecting the immunity in barley. Therefore, the inhibition of enzyme activity in C. sinensis increased with the increase in the stress concentration of Pb²⁺.
Key words:Cyclina sinensisPb²⁺acute toxicityimmune-related enzyme





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