黄凯旋, 陈亨, 徐帅帅, 刘莎莎, 吕颂辉
暨南大学 赤潮与海洋生物学研究中心, 广东 广州 510632

摘要:
利用快速光响应曲线（RLCs）技术研究了不同光背景下的底栖甲藻Coolia tropicalis的光合作用特性，比较了RLCs的不同序列（正序、反序和非序）和不同光照时间（10，30和60 s）对光合参数的影响。结果表明：正序RLCs由于序列内非光化学淬灭（NPQ）的快速累积，造成曲线的不饱以及光合参数最大相对电子传递速率（rETR_max）和半饱和光强（E_k）的高估。反序和非序RLCs均出现光饱和或光抑制，步长30 s和60 s的光合参数在不同光背景下的趋势基本一致。在底栖甲藻的光合生理研究中，可考虑采用步长30 s的反序RLCs进行测定。
关键词:叶绿素荧光快速光响应曲线底栖甲藻非光化学淬灭光合生理参数
DOI：10.11759/hykx20190505002
分类号:Q178.53
基金项目:国家自然科学青年基金项目（41706126）

Application of optimized rapid light curves in the study of photoacclimation characteristics of benthic dinoflagellates
HUANG Kai-xuan, CHEN Heng, XU Shuai-shuai, LIU Sha-sha, LV Song-hui
Research Center for Harmful Algae and Marine Biology, Jinan University, Guangzhou 510362, China
Abstract:
To obtain a better understanding of the photoacclimation of benthic dinoflagellates under different light history, we compared the construction (up-, down-, and non-sequences) and duration (10s, 30s, and 60s) of rapid light curves (RLCs) to reflect the photosynthetic status of the benthic dinoflagellate, Coolia tropicalis, under six gradients of light history. Results demonstrated that curves with up-sequence failed to saturate, and their maximal rETR (rETR_max) and light saturation coefficient (E_k) were overrated due to non-photochemical quenching rapidly accumulating during the sequence. In contrast, curves obtained using the durations of 30s and 60s with down- and non-sequences exhibited saturation or photoinhibition, and their parameters showed similar trends under six light gradients. Therefore, a duration of 30s with down-sequence RLCs can be used for the photosynthetic study of benthic dinoflagellates.
Key words:chlorophyll fluorescencerapid light curvesbenthic dinoflagellatesnon-photochemical quenchingphotosynthetic parameter





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