王可可¹, 田野¹, 姜源庆², 李培峰¹, 刘春颖¹, 杨桂朋¹
1.中国海洋大学 化学化工学院, 山东 青岛 266100;2.国家海洋局 烟台海洋环境监测中心站, 山东 烟台 264006

摘要:
基于全球变暖的背景，研究了温度对三角褐指藻（Phaeodactylum tricornutum）、小新月菱形藻（Nitzschia closterium f. minutissima）和中肋骨条藻（Skeletonema costatum）生长的影响，并初步探讨了温度对海洋硅藻生长过程中吸收或释放一氧化氮（NO）的影响。培养温度分别为20℃和25℃。结果显示，温度升高，中肋骨条藻的比生长速率降低（P < 0.05），而对三角褐指藻和小新月菱形藻的生长没有明显影响。不同种类硅藻对高温的适应能力不同，且在不同生长阶段对NO呈现不同的吸收或释放规律。与20℃培养条件相比，25℃条件下三角褐指藻对NO的净吸收速率在指数生长期、生长平台期和衰亡期分别升高了40.2%、98.2%和16.0%；小新月菱形藻对NO的净吸收速率在生长的平台期升高了5.7倍；中肋骨条藻对NO的净释放速率在生长的平台期升高幅度最大，为11.5倍。结果表明全球变暖对不同种类的硅藻代谢NO的促进程度不同。
关键词:硅藻温度一氧化氮(NO)吸收释放
DOI：10.11759/hykx20190506001
分类号:X55
基金项目:国家自然科学基金（41676065）；国家重点研发计划（2016YFA0601301）；中央高校基本科研业务费专项（201762032）

Effect of temperature on the uptake and release of nitric oxide by marine diatoms
WANG Ke-ke¹, TIAN Ye¹, JIANG Yuan-qing², LI Pei-feng¹, LIU Chun-ying¹, YANG Gui-peng¹
1.College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao 266100, China;2.Yantai Marine Environmental Monitoring Center Station, State Oceanic Administration, Yantai, Shandong 264006, China
Abstract:
In the context of global warming, the effect of temperature on the growth of Phaeodactylum tricornutum, Nitzschia closterium, and Skeletonema costatum were studied. The effect of temperature on the uptake and release of nitric oxide (NO) by marine diatoms during growth was also discussed. Diatom culture experiments were conducted at 20℃ and 25℃. The results showed that when the temperature increased, the specific growth rates of S. costatum decreased (P < 0.05); however, there was no significant influence on the growth of P. tricornutum and N. closterium. Different kinds of diatoms presented different levels of adaptability to high temperature and had different uptake or release patterns of NO at the various growth stages. Compared with the culture condition of 20℃, the net uptake rates of NO from P. tricornutum increased by 40.2%, 98.2%, and 16.0% during the exponential growth phase, the plateau period, and the decay period, respectively, under the culture condition of 25℃. The net uptake rates of NO from N. closterium increased by a factor of 5.7 during the plateau period, and the NO net release rates showed a maximum increase during the plateau period of S. costatum, which increased by a factor of 11.5 at 25℃. This study shows that global warming promotes different levels of NO metabolism in different types of marine diatoms.
Key words:diatomtemperaturenitric oxide (NO)uptakerelease





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