付新华¹, 刘国宁², 孙凤祥¹, 辛鹿¹, 陈倩倩¹
1.潍坊医学院 生物化学与分子生物学教研室, 山东 潍坊 261053;2.潍坊市环境监控中心, 山东 潍坊 261041

摘要:
为系统地了解莱州湾单环刺螠（Urechis unicinctus）遗传多样性，作者基于线粒体COI序列利用基因测序技术研究了莱州湾3个单环刺螠不同群体的遗传多样性。实验结果显示，84个莱州湾单环刺螠样本有变异位点数125个，其中包括43个单一变异位点，82个简约信息位点；48个单倍型，单倍型多样性0.977；核苷酸多样性指数0.01048；平均核苷酸差异数量13.725；莱州湾单环刺螠遗传多样性水平较高；个体聚类并没有体现出和地理位置相关。
关键词:莱州湾单环刺螠(Urechis unicinctus)线粒体COI遗传多样性
DOI：10.11759/hykx20190510001
分类号:X55
基金项目:山东省渤海海洋生态修复及能力建设项目（20140601）；潍坊医学院博士基金（2017BSQD46）

Analysis of the genetic diversity of Urechis unicinctus in Laizhou Bay based on the mitochondrial COI gene sequence
FU Xin-hua¹, LIU Guo-ning², SUN Feng-xiang¹, XIN Lu¹, CHEN Qian-qian¹
1.Weifang Medical University, Department of Biochemistry and Molecular Biology, 261053, China;2.Weifang Environmental Monitoring Center, 261041, China
Abstract:
Urechis unicinctus has a unique taxonomic status and a broad economic prospect. The mitochondrial COI gene sequences of U. unicinctus from three populations in Laizhou Bay of Shandong province were evaluated to analyze the genetic diversity of this species. A total of 70 pieces of fragments with a length of 1 309 bp were obtained in this analysis. There were 115 mutation sites in U. unicinctus, including 43 single mutation loci, 82 minimalism information loci, 77 double mutation minimalism information loci, and 5 triple mutation loci. The diversity index of the haplotypes was 0.977, and the nucleotide diversity index was 0.01048. The mean difference in the number of nucleotides was 13.725. These results indicate that U. unicinctus in Laizhou Bay has a high genetic diversity. The findings of this study provide basic information that could be useful for the conservation and development of U. unicinctus in Laizhou Bay of Shandong province.
Key words:Laizhou BayUrechis unicinctusmitochondrial COIgenetic diversity





PDF全文下载地址:

点我下载PDF