宋宗诚¹, 吴志昊^2,3, 王丽娟^2,3, 胡金伟², 岳新璐¹, 尤锋^2,3
1.威海圣航水产科技有限公司, 山东 威海 264200;2.中国科学院实验海洋生物学重点实验室, 海洋大科学研究中心, 山东 青岛 266071;3.青岛海洋科学与技术试点国家实验室, 海洋生物学与生物技术功能实验室, 山东 青岛 266000

摘要:
大菱鲆（Scophthalmus maximus）是我国重要的海水养殖鱼种，三倍体诱导对其种质改良、产业提升和生态安全具有重要的价值。目前有关大菱鲆三倍体的人工诱导虽已有报道，但对其诱导条件缺乏细致的研究。本文采用冷休克法抑制第二极体排放，通过对诱导处理起始时刻、温度和持续时间3个主要因素的单因子实验，筛选其最佳诱导条件。结果显示，在受精和孵化水温为15.0±0.5℃条件下，受精后6 min，用-2~-1℃处理25 min可获得较好的诱导效果，流式细胞仪检测其诱导率可达100%。随后在最适条件下进行了规模化诱导，获得鱼苗200万尾以上，其诱导率高于98%。跟踪观察发现，三倍体诱导组胚胎的形态及孵化时间与二倍体对照组基本一致。本研究结果可为大菱鲆三倍体的产业化应用提供基础。
关键词:大菱鲆三倍体冷休克规模化
DOI：10.11759/hykx20190903002
分类号:S917.4
基金项目:国家自然科学基金项目（31502156，31772834）；2017年山东省重点研发计划（公益类专项）项目（2017GHY15115）；山东省农业科技园产业提升工程（2017YQ004）

Optimization of artificial induction conditions and large-scale induction of the triploid turbot Scophthalmus maximus
SONG Zong-cheng¹, WU Zhi-hao^2,3, WANG Li-juan^2,3, HU Jin-wei², YUE Xin-lu¹, YOU Feng^2,3
1.Shenghang Aquatic Science and Technology Co. Ltd., Weihai 264200, China;2.Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;3.Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology(Qingdao), Qingdao 266000, China
Abstract:
The turbot (Scophthalmus maximus) is an important marine fish cultured in China. Induction of triploidy in the turbot would be of significant value to germplasm improvement, industrial upgradation, and ecological safety. Although in some studies, artificial induction of triploidy in the turbot has been investigated so far, the induction parameters remain uncertain. In this study, triploidy was induced in the turbot through cold shock to inhibit the emission of the second polar body. A single-factor experiment was conducted to screen for the optimal initiation time, temperature, and duration of treatment. The optimal initiation time for cold shock was found to be 6 min after fertilization at 15.0℃ ±0.5℃. The optimal treatment temperature and treatment duration were approximately from -2℃ to 1℃ and 25 min, respectively, and the induction rate could reach 100%. These optimal induction conditions were then used to induce triploid larvae on a large scale, and >2 million larvae were obtained. The induction rate was found to be 98%~100%. The morphology and hatching period of embryos in the triploid induction groups were essentially the same as those of embryos in the diploid control groups. These results would provide a basis for the industrial application of the triploid turbot.
Key words:turbottriploidcold shocklarge-scale





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