施琳妮^1,2, 于永翔², 姜勇³, 张正², 王印庚², 廖梅杰², 荣小军²
1.上海海洋大学水产与生命学院 上海 201306;2.中国水产科学研究院 黄海水产研究所, 青岛海洋科学与技术国家实验室, 海洋渔业科学与食品产出过程功能实验室, 青岛 266071;3.青岛国家海洋科学研究中心, 山东 青岛 266071

摘要:
美人鱼发光杆菌美人鱼亚种（Photobacterium damselae subsp. damselae）可感染多种海水养殖动物发病，为研究不同菌株之间的表型差异性，作者对不同来源的25株美人鱼发光杆菌美人鱼亚种菌株的溶血特性、生理生化特征、药物敏感性以及5种最敏感抗生素的最小抑菌浓度进行了分析。结果表明：（1）25株菌株在TSB培养基上均为白色不透明圆形菌落，TCBS培养基上菌落显绿色，直径为1~2 mm，革兰氏染色均为阴性；（2）25株菌对3%胰胨水、无盐胰胨水、葡萄糖、肌醇、3% NaCl、ONPG以及精氨酸双水解酶等22种生化反应呈现出高度一致性，对于丙二酸盐、赖氨酸脱羧酶、尿素酶以及西蒙氏枸橼酸盐表现出明显差异；（3）所有菌株中仅有2株菌表现出β溶血，23株菌表现出α溶血；（4）25株菌对丁胺卡那、链霉素、复方新诺明、乙酰螺旋霉素以及苯唑青霉素5种抗生素均表现出耐药性；对萘啶酸、氟罗沙星、菌必治、头孢胺噻肟、盖伯斯林以及洛美沙星6种抗生素均表现敏感性；对恩诺沙星等27种抗生素的药物敏感性存在显著差异；（5）5种抗生素的MIC检测结果表明，25株菌对萘啶酸以及环丙沙星的MIC基本一致，浓度分别是2.5~10 μg/mL以及低于2.5μg/mL，对多粘菌素B、氧氟沙星以及洛美沙星3种抗生素差异较大。通过本研究证实不同来源的美人鱼发光杆菌美人鱼亚种菌株表型特征存在明显差异。
关键词:美人鱼发光杆菌美人鱼亚种(Photobacterium damselae subsp. damselae)溶血性抗生素耐药生理生化特征
DOI：10.11759/hykx20190115001
分类号:S947
基金项目:山东省重大科技创新工程项目（2018YFJH0730）

Studies on the phenotypic differences of different Photobacterium damselae subsp. damselae strains
SHI Lin-ni^1,2, YU Yong-xiang², JIANG Yong³, ZHANG Zheng², WANG Yin-geng², LIAO Mei-jie², RONG Xiao-jun²
1.College of Fishers and Life Science, Shanghai Ocean University, Shanghai 201306, China;2.Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao, National Laboratory for Marine Science and Technology, Qingdao 266071, China;3.National Marine Science Research Center, Qingdao 266071, China
Abstract:
Photobacterium damselae subsp. damselae is an important pathogen to cause different kinds of marine animal disease. In this study, 25 strains of this bacterium which isolated from different host and region were selected to analyze their difference of physiology, biochemistry, drug resistance and MIC of 5 antibiotic. The results showed that different phenotypic characteristics existed among the 25 strains. They are:(1) All these strains formed whites and opaque colonies on TSB agar and green colonies on TCBS medium with diameter 1-2mm, and they were all Gram-negative (2) There were 22 biochemical reactions characteristics similar among the 25 strains, including 3% peptone water, salt-free peptone water, glucose, inositol, 3% NaCl, ONPG and arginine dihydrolase et al. The 4 biochemical reaction of malonate, urease, lysine decarboxylase and simmont citrate were different. (3) Only 2 strains showed beta hemolysis, the others 23 strains showed alpha hemolysis,. (4) All 25 strains showed resistance to 5 antibiotics of Amikacin, Streptomycin, SMZ-TMP, Acetylspiramycin and Oxacillin, and susceptivity to 6 antibiotics of Nalidixic acid, Fleroxacin, Cetriaxone Sodium, Cefotaxime, Ceftizoxime and Lomefloxacin. The differences of drug susceptivity existed in the others 27 antibiotics including Enrofloxacin and so on. (5)The MIC of these 25 strains were almost the same to 2 antibiotics of Nalidix acid and Ciprofloxacin, but significant difference to 3 antibiotics of Polymyxin B, Ofloxacin and Lomefloxacin. This study confirmed that the phenotypic characteristics of Photobacterium damselae subsp. damselae strains which isolated from different host and region were significantly different.
Key words:Photobacterium damselae subsp. damselaeHemolysisPhenotypic characteristics





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