李璐^1,2,3,4, 张朋艳^1,2,3,4, 姚建亭^1,2,3, 段德麟^1,2,3
1.中国科学院海洋研究所, 山东 青岛 266071;2.中国科学院实验海洋生物学重点实验室, 山东 青岛 266071;3.青岛海洋科学与技术试点国家实验室实验海洋生物学与生物技术实验室, 山东 青岛 266071;4.中国科学院大学, 北京 100049

摘要:
利用cDNA末端快速克隆（RACE）技术，获得海带（Saccharina japonica） CRY-DASH基因（SjCRY-DASH）全长序列，结构分析发现，其ORF区长1779 bp，编码592个氨基酸。进行氨基酸同源序列比对，其与其他藻类和高等植物间存在两个重要辅基MTHF和FAD结合的保守域。通过不同光质照射诱导海带幼孢子体，发现蓝光、白光诱导1 h后，均能使SjCRY-DASH转录水平上升，且SjCRY-DASH对蓝光的响应更强烈。本研究结果为研究大型褐藻-海带CRY-DASH受光诱导调控功能打下基础。
关键词:蓝光海带(Saccharina japonica)CRY-DASH基因孢子体
DOI：10.11759/hykx20170222003
分类号:S917.3
基金项目:国家海洋公益性行业科研专项（201405040）；青岛海洋科学与技术国家实验室计划项目（2015ASKJ02）

Cloning and transcriptional analysis of CRY-DASH from Saccharina japonica (Laminariales, Phaeophyceae)
LI Lu^1,2,3,4, ZHANG Peng-yan^1,2,3,4, YAO Jian-ting^1,2,3, DUAN De-lin^1,2,3
1.Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;2.CAS Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;3.Marine Biology and Biotechnology Laboratory, Pilot National Laboratory for Marine Science and Technology(Qingdao), Qingdao 266071, China;4.University of Chinese Academy of Sciences, Beijing 100049, China
Abstract:
In the present work, we characterized the full-length cDNA of CRY-DASH from Saccharina japonica (designated as SjCRY-DASH) using the rapid amplification of cDNA ends technique. The open reading frame length of SjCRY-DASH was 1779 bp, and the gene encoded a protein of 592 amino acids. Using multiple alignments of protein sequences, conserved residues interacting with two crucial cofactors, MTHF and FAD, were detected between SjCRY-DASH and several DASH-type proteins of other algae and higher plants. Juvenile sporophytes were induced by light of different wavelengths. Both blue and white light upregulated the transcriptional level of SjCRY-DASH within 1 h, although the gene showed stronger responses to blue light than to white light. Our results lay a foundation for future studies on light induction and regulation by CRY-DASH of S. japonica.
Key words:blue lightSaccharina japonicaCRY-DASHsporophytes





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