宋琦^1,2, 李晓明¹, 胡雪怡¹, 杨遂群¹, 王斌贵^1,3
1.中国科学院海洋研究所 实验海洋生物学重点实验室, 山东 青岛 266071;2.中国科学院大学, 北京 100049;3.中国科学院海洋大科学研究中心, 山东 青岛 266071

摘要:
对海鞘内生真菌棒曲霉Aspergillus clavatus AS-107的次级代谢产物进行了化学研究。利用反相和正相硅胶柱层析、制备薄层层析（pTLC）以及葡聚糖凝胶柱层析等色谱方法从其发酵产物中分离得到7个喹唑啉酮生物碱类单体化合物，并综合其理化性质和波谱数据鉴定了它们的化学结构，分别是norquinadoline A（1）、quinadoline A（2）、epi-fiscalin D（3）、fiscalin B（4）、quinadoline B（5）、prelapatin B（6）和tryptoquivalines L（7）。对所有化合物进行了抗水产致病菌实验，测试结果表明化合物2对嗜水气单胞菌（Aeromonas hydrophilia）具有一定的抑制作用，其MIC值为16 μg/mL；化合物6对副溶血性弧菌（Vibrio parahaemolyticus）、哈氏弧菌（V.harveyi）和嗜水气单胞菌（Aeromonas hydrophilia）有一定的抑制活性，其MIC值分别为16、32和32 μg/mL。
关键词:海鞘内生真菌次级代谢产物生物碱抗菌活性
DOI：10.11759/hykx20190321005
分类号:O629
基金项目:国家自然科学基金委员会-山东省海洋科学研究中心联合基金（U1606403）

Chemical constituents of an endophytic fungus derived from ascidian Aspergillus clavatus AS-107
SONG Qi^1,2, LI Xiao-ming¹, HU Xue-yi¹, YANG Sui-qun¹, WANG Bin-gui^1,3
1.Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;2.University of the Chinese Academy of Sciences, Beijing 100049, China;3.Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China
Abstract:
Chemical investigation of Aspergillus clavatus AS-107, an endophytic fungus derived from ascidian, was performed. Seven alkaloids were obtained from the fermentation products of the strain by preparative TLC, Sephadex LH-20 and column chromatography with silica gel. Structures of the seven compounds were determined by analysis of their physical and chemical properties and spectroscopic data to be norquinadoline A (1), quinadoline A (2), epi-fiscalin D (3), fiscalin B (4), quinadoline B (5), prelapatin B (6), and tryptoquivalines L (7). Antimicrobial experiments showed that compound 2 has potent antimicrobial activity against Aeromonas hydrophilia with an MIC value of 16 μg/mL. Compound 6 has a certain inhibitory activity against Vibrio parahaemolyticus, V. harveyi, and Aeromonas hydrophilia, with MIC values of 16, 32, and 32 μg/mL.
Key words:ascidianendophytic fungussecondary metabolitealkaloidantimicrobial activity





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