吴素煌^1,2,3, 岳洋^1,3, 王晶^1,3, 张全斌^1,3
1.中国科学院 海洋研究所 实验海洋生物学重点实验室, 山东 青岛 266071;2.中国科学院大学, 北京 100049;3.中国科学院 海洋大科学研究中心, 山东 青岛 266071

摘要:
海带采用乙醇提取色素，通过柱层析分离纯化其中的岩藻黄素，并最终通过高效制备液相色谱制备了三种岩藻黄素的异构体。通过紫外光谱（UV）、核磁共振氢谱（¹H-NMR）以及质谱（MS）对岩藻黄素及其异构体进行了结构表征和鉴定。采用乳腺癌细胞MDA-MB-231模型，对岩藻黄素的体外抗肿瘤活性进行了研究。结果表明，岩藻黄素对乳腺癌细胞生长有显著的抑制作用，其中13-顺和13'-顺岩藻黄素组分对癌细胞的抑制活性最强，IC₅₀值达到23.71 μg/mL。全反式岩藻黄素和9'-顺岩藻黄素两种异构体IC₅₀值分别为45.30和27.09 μg/mL。qPCR试验表明岩藻黄素各异构体可以通过抑制癌细胞NF-κB家族基因表达而抑制乳腺癌细胞生长。
关键词:岩藻黄素顺反异构体乳腺癌抗肿瘤
DOI：10.11759/hykx20190308001
分类号:R284
基金项目:山东省重点研发计划项目（2016HYSP0002）；中国科学院王宽诚率先人才计划产研人才扶持项目

Isolation, identification and the in vitro anti-breast cancer effects of fucoxanthin stereoisomers from Laminaria japonica
WU Su-huang^1,2,3, YUE Yang^1,3, WANG Jing^1,3, ZHANG Quan-bin^1,3
1.Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;2.University of Chinese Academy of Sciences, Beijing 100049, China;3.Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China
Abstract:
Pigments from Laminaria japonica were extracted using ethyl acetate, and fucoxanthin stereoisomers were purified by column chromatography. Finally, three fucoxanthin stereoisomers were prepared by preparative high performance liquid chromatography. The structures of the fucoxanthin stereoisomers were characterized by ultraviolet (UV) spectroscopy, mass spectrometry (MS), and nuclear magnetic resonance (NMR). The in vitro antitumor activity of the fucoxanthin stereoisomers was analyzed using MDA-MB-231 breast cancer cell line. Results demonstrated that the fucoxanthin stereoisomers possessed significant inhibitory effects against the growth of breast cancer cells. The 13-cis and 13'-cis fucoxanthin mixtures exhibited the highest inhibitory activity, with the IC₅₀ value being 23.71 μg/mL. Moreover, the IC₅₀ values of all trans-fucoxanthin and 9'-cis fucoxanthin mixtures were 45.30 and 27.09 μg/mL, respectively. Furthermore, the qPCR results suggested that the fucoxanthin stereoisomers inhibit the growth of breast cancer cells by downregulating the expression of the NF-κB pathway-related genes.
Key words:fucoxanthinstereoisomerbreast cancerantitumor activity





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