蒋南芳¹, 李炳玺¹, 李伟达¹, 刘效磊², 王晓倩¹, 常新涛³, 綦慧敏¹
1.潍坊医学院药学院, 山东 潍坊 261053;2.潍坊学院, 山东 潍坊 261061;3.中共莱芜市委市直机关工作委员会, 山东 莱芜 271100

摘要:
利用多糖结构修饰原理，制备磷酸化孔石莼（Ulva pertusa）多糖（PU），并对其进行体外抗氧化活性研究。将多聚磷酸钠与三偏磷酸钠混合后作为磷酸化反应试剂，与孔石莼多糖U反应制备不同磷酸根含量的孔石莼多糖PU。通过磷元素含量分析，确定成功制备不同磷酸根含量的孔石莼多糖衍生物PU，并测定不同磷酸根含量的孔石莼多糖PU与孔石莼多糖U对羟自由基、超氧阴离子清除能力以及金属螯合能力。实验发现通过本方法制备得到的PU6磷酸根含量最高为10.47%。综上所述，不同磷酸根含量的孔石莼多糖体外抗氧化活性不同，与孔石莼多糖U相比，磷酸根含量最高的PU6表现出最强的羟自由基清除作用。在100 μg/mL多糖浓度下，PU6表现出最强的金属鳌合能力。
关键词:孔石莼(Ulva pertusa)多糖磷酸化抗氧化活性
DOI：10.11759/hykx20180720001
分类号:R914
基金项目:国家自然科学基金项目（4120612/D0619）；山东省自然科学基金（ZR2012DL13）；药食同源植物资源开发四川省高校重点实验室开放基金资助（10Y201808）

Antioxidant activity of different phosphate content derivatives of polysaccharide extracted from Ulva pertusa in vitro
JIANG Nan-fang¹, LI Bing-xi¹, LI Wei-da¹, LIU Xiao-lei², WANG Xiao-qian¹, CHANG Xin-tao³, QI Hui-min¹
1.College of pharmacy, Weifang Medical University, Weifang 261053, China;2.Weifang University, Weifang 261061, China;3.work committee for offices directly under the CPC Laiwu municipal committee, Laiwu 271100, China
Abstract:
The phosphorylated derivative of polysaccharide from Ulva pertusa(PU) was prepared by the principle of polysaccharide structure modification. Its antioxidant activity was studied in vitro. Sodium tripolyphosphate and sodium trimetaphosphate were used as phosphorylating reagents. Then, PUs with different phosphate contents were prepared by mixing the polysaccharide from Ulva pertusa (U) and phosphorylating reagents with different conditions. The phosphorus contents of PUs were determined by elemental analysis. PU6 featured the highest phosphate content of 10.47%. We determined the hydroxyl radical scavenging activity, superoxide radical scavenging activity, and metal chelating power of U and PUs. The results indicate that PUs with different phosphorus contents exhibit varying antioxidant activities. Compared with U, PU6 with the highest phosphate content showed a stronger hydroxyl radical activity. PU6 showed the strongest metal chelating power at a concentration of 100 μg/mL.
Key words:Ulva pertusapolysaccharidesphosphorylationantioxidant activity





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