潘 滢¹, 郑利兵¹, 王 军¹
厦门大学 海洋与地球学院

摘要:
简单重复序列中间区域(inter-simple sequence repeat, ISSR)是一种微卫星类分子标记。为明确条纹斑竹鲨(Chiloscyllium plagiosum)中国群体的遗传多样性和遗传关系, 作者利用ISSR技术对福建厦门(XM)、福建平潭(PT)、广东湛江(ZJ)、海南海口(HK)和台湾(TW)5个土著地理群体的条纹斑竹鲨进行了遗传多样性和遗传关系分析。从56对ISSR引物中筛选出13对多态性引物, 扩增共获得81个重复性的位点, 其中多态性位点57个, 多态位点百分率为70.37%。5个群体的多态位点百分率、Nei’s基因多样性和Shannon’s信息指数分别为38.27%～58.02%, 0.1353～0.2155和0.2032～0.3193。UPGMA聚类分析表明PT 群体首先和XM群体聚类, 再与TW群体聚类, 最后与ZJ群体和HK群体形成的分支聚类, 即形成了与地理距离远近相关的基因交流模式。
关键词:条纹斑竹鲨(Chiloscyllium plagiosum)ISSR遗传多样性地理群体
DOI：10.11759/hykx20170925003
分类号:
基金项目:国家自然科学基金项目(40876080)

Genetic structure analysis of five populations of Chiloscyllium plagiosum using inter-simple sequence repeat molecular markers
PAN Ying,ZHENG Li-bing,WANG Jun
Abstract:
Inter-simple sequence repeat (ISSR) is a molecular marker of microsatellite. In this study, the genetic diversity and the genetic relationship of five native populations of the whitespotted bamboo shark Chiloscyllium plagiosum sampled from Xiamen (XM) and Pingtan (PT) in Fujian, Zhanjiang (ZJ) in Guangdong, Haikou (HK) in Hainan, and Taiwan (TW) were analyzed using the ISSR technique. A total of 13 polymorphic primers were selected from 56 ISSR primers. Using PCR amplification, 81 loci with great reproducibility, including 57 polymorphic loci, were acquired, indicating 70.37% of polymorphic loci. The percentage of polymorphic loci, the Shannon diversity indices, and the Nei’s gene diversity values of the five populations were 38.27%–58.02%, 0.1353–0.2155, and 0.2032–0.3193, respectively. The UPGMA tree constructed using the MEGA5.0 software showed that PT and XM populations clustered together initially and then clustered with TW population and finally joined to the branch clustered by ZJ and HK populations, which resulted in the gene exchange pattern correlating to the geographical distance.
Key words:Chiloscyllium plagiosuminter-simple sequence repeat (ISSR)genetic diversitygeographical populations





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