刘 慧¹, 杨振男¹, 刘 岚^1,2, 陈碧双^1,2
1.中山大学 海洋科学学院;2.中山大学 南海资源开发与保护协同创新中心

摘要:
研究了海洋真菌介导邻-、间-、对- 溴苯乙酮的不对称还原反应。具体考察了Rhodotorulamucilageinosa GIM 2.157 对邻-、间-、对- 溴苯乙酮的催化活性和对映选择性, 并综合考察了反应温度、pH、反应时间和底物浓度等因素对还原产物的产率及立体选择性的影响。确定最佳反应条件: 反应温度为25℃, 反应体系的pH 为7, 反应时间为24 h, 底物浓度为10 mmol/L。在最佳条件下, 产率以及立体选择性均>99%。
关键词:海洋真菌邻-、间-、对- 溴苯乙酮不对称还原生物催化
DOI：10.11759/hykx20170711002
分类号:
基金项目:高校基本科研业务费中山大学青年教师培育项目(17lgpy58);国家自然科学基金(41706148); 广东省自然科学基金(2017A030310232);海洋经济创新发展区域示范专项(2018ZX09735010)

Asymmetric bioreduction of o-, m-, and p-bromo acetophenone by whole cells of marine-derived fungi
LIU Hui,YANG Zhen-nan,LIU Lan,CHEN Bi-Shuang
Abstract:
In this study, the catalytic activity and enantioselectivity of Rhodotorula mucilageinosa GIM 2.157 toward the bioreduction of o-, m-, and p-bromoacetophenone to chiral alcohols were investigated. The effects of reaction temperature, pH, reaction time, and substrate concentration on the yield and enantioselectivity of the product, (S)-1-(4-bromophenyl)ethanol, were evaluated. The determined optimum reaction conditions are as follows: 25℃ reaction temperature, reaction system pH of 7, 24 h reaction time, and 10 mmol/L substrate concentration. Under the optimum conditions, the desired chiral alcohols were achieved with yield and ee up to > 99%.
Key words:marine fungio-m-p-bromo acetophenoneasymmetric bioreductionbiocatalysis





PDF全文下载地址:

点我下载PDF