李 宁^1,2,3, 郭传龙^1,2,3, 王立军^1,2, 李晓伟^1,2,3, 刘 华^1,2,3, 徐 琦^1,2,3, 史大永^1,2,3
1.中国科学院 实验海洋生物学重点实验室 中国科学院 海洋研究所;2.海洋药物与生物制品功能实验室, 青岛海洋科学与技术国家实验室;3.中国科学院大学

摘要:
为合理利用海洋资源、探究海昆桑(昆布-桑叶-山药-枸杞-肉桂复合)提取物的降血糖作用, 通过腹腔注射链脲佐菌素STZ构建2型糖尿病大鼠模型, 随机分为模型组、阳性对照组、海昆桑乙醇提取物低、中、高剂量组以及水提物高剂量组, 分别以0.5% CMC-Na液、二甲双胍0.08 g/(kg·d)、海昆桑乙醇提取物0.2 g/(kg·d)、0.4 g/(kg·d)、0.8 g/(kg·d)、海昆桑水提物0.8 g/(kg·d)灌胃, 另随机取10只正常大鼠作为正常对照组(0.5% CMC-Na 液), 持续给药4 周。给药后每周对各组大鼠的一般情况变化进行分析, 并利用其模型对海昆桑提取物进行空腹血糖试验以及糖耐量试验。结果显示, 海昆桑提取物可以改善2 型糖尿病高血糖症状, 且以乙醇提取物高剂量组降血糖作用为最佳(P<0.01)。实验结果说明,海昆桑提取物具有降低血糖的作用, 为降糖药物的研发或海洋保健食品的研制提供了新途径。
关键词:海洋资源海昆桑提取物降血糖2 型糖尿病
DOI：10.11759/hykx20171115001
分类号:
基金项目:山东省重点研发计划(2016GSF115009)

Hypoglycemic activity of Haikunsang mixture on rats with type 2 diabetes mellitus
LI Ning,GUO Chuan-long,WANG Li-jun,LI Xiao-wei,LIU Hua,XU Qi,SHI Da-yong
Abstract:
To investigate the potential for utilizing a marine resource, we investigated the hypoglycemia activity of extracts derived from a mixture of Haikunsang in rats with type 2 diabetes mellitus. We divided the streptozotocin (STZ)-induced rats into a model group, positive control group, and low, medium, and high dose groups of alcoholic extracts of Haikunsang, as well as a high dose group of aqueous extracts of Haikunsang. These groups were fed by gavage with 0.5% CMC-Na, 0.08 g·kg^-1·d^-1 metformin, 0.2, 0.4, 0.8 g·kg^-1·d^-1 alcoholic extracts of Haikunsang, and 0.8 g·kg^-1·d^-1 aqueous extracts of Haikunsang. As a normal control group, we randomly selected another 10 healthy rats, which were fed by gavage with 0.5% CMC-Na. We consecutively treated both the diabetic and healthy rats for 4 weeks, and individually recorded their body weights, food intakes, psychiatric status, and other changes. We found the diabetic rats to exhibit typical symptoms. After Haikunsang extract administration for 4 weeks, we recorded their blood glucose concentrations via an oral glucose tolerance test (OGTT). We found the blood glucose concentrations of most rats had significantly decreased in the high dose groups that had received alcoholic extracts of Haikunsang (0.8 g·kg^-1·d^-1, P<0.01). These results suggest that extracts derived from a mixture of Haikunsang play a regulatory role in the blood glucose levels of diabetic rats, thereby providing a new approach to the development of hypoglycemic drugs and marine health foods.
Key words:marine resourceHaikunsangextractshypoglycemia activitytype 2 diabetes mellitus





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