石 祥¹, 肖 容¹, 邱立国¹, 周海龙¹
海南大学 热带农林学院

摘要:
凡纳滨对虾(Litopenaeus vannamei)是世界上最重要的对虾养殖品种之一。为比较凡纳滨对虾不同生长发育时期应答氨氮胁迫的敏感性差异, 作者对其不同生长发育时期24 h半数致死浓度(LC₅₀)进行测定, 并分析其胁迫效应。结果表明, 24 hLC₅₀值在虾的不同生长发育时期差异显著(P<0.05), 不同时期凡纳滨对虾的LC₉₀与LC₅₀变化趋势相同。在溞状幼体Ⅱ期(Z2)LC₅₀值最低, 为17.811 mg/L, 在仔虾第V期(P5)时, LC₅₀值最高, 为44.808 mg/L。根据LC₅₀和LC₉₀的变化趋势可以看出, 仔虾比幼体期的耐受性强。本试验结果不仅为凡纳滨对虾不同生长发育时期的苗种培育提供了重要的基础数据,而且为凡纳滨对虾耐氨氮品系选育提供了重要指导。
关键词:凡纳滨对虾氨氮胁迫不同生长发育时期敏感性
DOI：10.11759/hykx20170723001
分类号:
基金项目:海南省自然科学基金项目(20164159); 国家自然科学基金项目(41161077)

Comparative study on sensitivity of ammonia nitrogen at different life stages of Litopenaeus vannamei
SHI Xiang,XIAO Rong,QIU Li-guo,ZHOU Hai-long
Abstract:
Litopenaeus vannamei is one of the world’s most commercially valuable shrimps. In this study, we conducted an ammonia nitrogen stress test on L. vannamei to estimate the 24-h half-lethal concentration (24 h LC₅₀) values at different larvae stages. The results showed that the 24 h LC₅₀ values were significantly different at different developmental stages of the shrimp (P < 0.05). Ammonia nitrogen stress had the same trend as LC₉₀ and LC₅₀ in L. vannamei at different developmental stages. LC₅₀ had the least value at Zoea II (17.811 mg/L) and the maximum value at Postlarvae V (44.808 mg/L). According to the trends of LC₅₀ and LC₉₀, we can conclude that the latter stage has higher tolerance than the earlier stage. These results can provide basic information for shrimp farming and nursery and can also guide selection breeding for the tolerance of L. vannamei. This study will play an important role in health development of shrimp in the shrimp industry.
Key words:Litopenaeus vannameiammonia nitrogen stressdifferent life stagessensitivity





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