姓名：李静

学历/职称/职务：博士/副教授/硕士生导师



联系方式：

邮箱：lijing313@ouc.edu.cn

电话：**;**

地址：鱼山路5号 中国海洋大学科学馆313



学习工作简历：

1992.9-1996.7 山东轻工业学院发酵工程 本科

1996.9-1999.7 无锡轻工大学生物工程学院 硕士

1999.7-2001.10 中国海洋大学精细化工研究所 从事微生物药物开发

2001.10-至今 中国海洋大学生命学院 副教授/硕士生导师

2013.8-2014.8 美国加州大学SANDIEGO分校SCRIPPS海洋研究所访问学者



科研领域描述：

放线菌作为海洋微生物中的一个重要类群，具有高度的物种多样性和功能多样性，更因其能产生独特的次生代谢产物而备受关注。我们主要利用宏基因组技术、基因勘探技术及系统分类学手段分析海洋微生物（放线菌）资源多样性（菌种、基因）及其功能，为海洋微生物资源的利用和开发提供基础。



研究方向：

（1）极端环境（极地、深远海）或特殊环境下（近海、滨海湿地）的放线菌资源开发及分子生态学、生物地理学研究；

（2）采用Genomemining挖掘菌株次级代谢产物合成的多样性，评估海洋放线菌合成活性物质的潜力，探讨其功能演化途径，发现海洋放线菌分类系统与次级代谢产物合成基因的相关性；

（3）海洋放线菌（化学）生态功能的探索；



目前主持和参加的科研课题：

1. 国家自然科学基金（青年基金）项目：南极乔治王岛陆地-潮间带-近海沉积物放线菌群落结构研究，主持

2. 山东省科技攻关项目：海洋药用放线菌的快速分离和活性筛选新技术研究，主持

3. 山东省自然科学基金：红树林放线菌中新型糖基转移酶基因的发现和克隆，主持

4. 青岛市科技发展计划项目：黄渤海海洋放线菌多样性及其次级代谢潜力发掘，主持

5. 中国海洋大学专项基金项目：南海海洋放线菌多样性及其次级代谢潜力发掘，主持

6. 国家自然科学基金（面上）：基因-活性-化学筛选指导下深海微生物活性次级代谢产物研究，第2位

7. 国家自然科学基金（青年基金）项目：海洋放线菌Streptomyces sp.CHQ-64次级代谢产物的结构及活性多样性研究，第2位

8. 南北极综合环境考察及资源潜力评估专项，参加

9. 国家高技术研究发展计划（863）：海洋生物候选药物成药性评价技术，参加

10. 国家高技术研究发展计划（863）：化学品生物过程转化过程与装备，参加



代表性成果：

发表论文：

1. Shanshan Xu, Lien Yan, Xuan Zhang, ChaoWang, Ge Feng, Jing Li*. Nocardiopsis fildesensis sp. nov., an actinomyceteisolated from Antarctica soil. Int J Syst Evol Microbiol, 2014(64): 174–179.SCI

2. Jing Li, Xin-Peng Tian, Tian-Jiao Zhu,Ling-Ling Yang, Wen-Jun Li. Streptomyces fildesensis sp. nov., a novelstreptomycete isolated from Antarctic soil. Antonie van Leeuwenhoek，2011(100):531-543.SCI

3. Jing Li, Zhenming Chi, XianghongWang.Cloning of the SAP6 gene of Metschnikowia reukaufii and its heterologousexpression and characterization in Escherichia coli. Microbiological Research,2010(165):173-182. SCI

4. Li Jing, Peng Ying, Wang Xianghong, ChiZhenming. Optimum production and characterization of an acid protease frommarine yeast Metschnikowia reukaufii W6b. Journal of Ocean University of China,2010(9):359-364.

5. Jing Li, Zhenming Chi, Zhiqiang Liu,Lixi Yue, Ying Peng, Lin Wang. Cloning and characterization of a novel asparticprotease gene from marine-derived Metschnikowia reukaufii and its expression inE. coli. Appl Biochem Biotechnol, 2009(159):119-132. SCI

6. Li Jing, Chi Zhenming, Wang Xianghong,Peng Ying, Chi Zhe. The selection of alkaline protease-producing yeasts frommarine environments and evaluation of their bioactive peptide production.Chinese Journal of Oceanology and Limnology, 2009 (27): 753-761. SCI

7. 冯鸽, 许姗姗, 王超, 严立恩, 张璇, 李静*. 黄河三角洲滨海湿地非培养放线菌多样性. 微生物学通报, 2013,40(9):1-10.

8. 孙艺华, 冯鸽, 王超, 许姗姗, 李静*. 红树林放线菌多样性及其新型糖苷类化合物合成潜力的发掘. 中国海洋药物, 2013,32(1):1-10.

9. Qian Che, Tianjiao Zhu, Xin Qi, AttilaMándi, Tibor Kurtán, Xiaomei Mo, Jing Li, Qianqun Gu, and Dehai Li. Hybridisoprenoids from a reeds rhizosphere soil derived actinomycete Streptomyces sp.CHQ-64. Organic Letters, 2012(14):3438-3441.

10. Xiumei Ni, Lixi Yue, Jing Li, ZhenmingChi, Zhiqiang Liu, Madzak Catherine. Properties of alkaline proteasegenetically engineered on cell surface of the yeast Yarrowia lipolytica. Indianjournal of biochemistry & biophysics, 2009(46):294-298. SCI

11. Weilin Wang, Zhenming Chi, Zhe Chi,Jing Li, Xianghong Wang. Siderophore production by the marine-derivedAureobasidium pullulans and its antimicrobial activity. Bioresource technology.2009(100):2639-2641. SCI

12. Fang Wang, Lixi Yue, Lin Wang,Catherine Madzak, Jing Li, Xianghong Wang, Zhenming Chi. Genetic modificationof the marine-derived yeast Yarrowia lipolytica with high-protein content usinga GPI-anchor-fusion expression system. Biotechnology progress.2009(25):1297-1303. SCI

13. Zhengming Chi, Jing Li, Lin Wang, SusuHe, Xianghong Wang. Pichia pastoris INO1 gene expression affects intracellularmaltase activity and MAL1+ gene expression in Schizosaccharomyces pombe. IndianJournal of Biochemistry and Biophysics, 2008(45): 174-178. SCI

14. Lixi Yue, Zhenming Chi, Lin Wang, JiaLiu, Catherine Madzak, Jing Li, Xianghong Wang. Construction of a new plasmidfor surface display on cells of Yarrowia lipolytica. Journal of MicrobiologicalMethods, 2008(72):116-123. SCI



教学：

第二副主编，参编《现代微生物生态学》，2010年1月出版，负责第二章的撰写。



CurriculumVitae



Name: LI JING

Title: Associate Professor, PhD.

Contact

Email：lijing313@ouc.edu.cn

Tel：**;**

Address：No.5 Yu Shan Road.K313



Experiences:

Education:

? Ocean University of China, College of Marine Life Sciences, Qingdao,P.R. China

PhD，Microbiology (2008); Area of Specialization: Marine microbiology

? Jiangnan University, School of Biotechnology, Wuxi, P.R. China

ME，BiologicalEngineering (1999); Area of Specialization: Fermentation

? Shandong Polytechnic University, School of Food &Engineering,Jinan, P.R. China

BE，Fermentation (1996);



Research:

Ocean University of China, College ofMarine Life Sciences, 2002-preaent

ScrippsInstitute of Oceanography, USA, Visiting Scholar, 2013.8-2014.8



Research Interests：

1. Actinobacterial diversity: Evaluate thediversity and distribution patterns of actinobacteria in extreme or specialecosystems by using culture-independent and culture-based approaches. Developnew cultivation techniques to isolate rare actinobacteria. Identify anddescribe novel species.

2. Evaluation of novel secondary metabolitebiosynthetic potential: Use a sequence-guided genetic screening strategy toclone and identify genes that encode PKS, NRPS or tailoring enzymes involved innatural product biosyntheses pathways. Use these genes as indicator for theidentification and preselection of strains that have the genetic potential toproduce novel secondary metabolite of interest.

3. Correlation between systematics andnatural products: Analyse the diversity and distribution of secondary metabolitesbiosynthetic genes in different actinobacteria taxa. Determine if there is ataxonomic pattern relative to biosynthetic genes.



Publications:

1. Shanshan Xu, Lien Yan, Xuan Zhang, ChaoWang, Ge Feng, Jing Li*. Nocardiopsis fildesensis sp. nov., an actinomyceteisolated from Antarctica soil. Int J Syst Evol Microbiol, 2014(64): 174–179.SCI

2. Jing Li, Xin-Peng Tian, Tian-Jiao Zhu,Ling-Ling Yang, Wen-Jun Li. Streptomyces fildesensis sp. nov., a novelstreptomycete isolated from Antarctic soil. Antonie van Leeuwenhoek，2011(100):531-543.SCI

3. Jing Li, Zhenming Chi, XianghongWang.Cloning of the SAP6 gene of Metschnikowia reukaufii and its heterologousexpression and characterization in Escherichia coli. Microbiological Research,2010(165):173-182. SCI

4. Li Jing, Peng Ying, Wang Xianghong, ChiZhenming. Optimum production and characterization of an acid protease frommarine yeast Metschnikowia reukaufii W6b. Journal of Ocean University of China,2010(9):359-364.

5. Jing Li, Zhenming Chi, Zhiqiang Liu,Lixi Yue, Ying Peng, Lin Wang. Cloning and characterization of a novel asparticprotease gene from marine-derived Metschnikowia reukaufii and its expression inE. coli. Appl Biochem Biotechnol, 2009(159):119-132. SCI

6. Li Jing, Chi Zhenming, Wang Xianghong,Peng Ying, Chi Zhe. The selection of alkaline protease-producing yeasts frommarine environments and evaluation of their bioactive peptide production.Chinese Journal of Oceanology and Limnology, 2009 (27): 753-761. SCI

7. Fneg Ge, Xu Shan-Shan, Wang Chao, YanLi-En, Zhang Xuan, Li Jing*. Actinobacterial diversity in a coastal wetland ofthe Yellow River Delta revealed by culture-independent approach. MicrobiologyChina, 2013,40(9):1-10.

8. Sun Yi-hua, Feng Ge, Wang Chao, XuShan-shan, Li Jing*. Phylogenetic diversity analysis and evaluating of novelglycosides biosynthetic potential of Mangrove actinomycetes. Chinese Journal ofMarine Drugs. 2013,32(1):1-10.

9. Qian Che, Tianjiao Zhu, Xin Qi, AttilaMándi, Tibor Kurtán, Xiaomei Mo, Jing Li, Qianqun Gu, and Dehai Li. Hybridisoprenoids from a reeds rhizosphere soil derived actinomycete Streptomyces sp.CHQ-64. Organic Letters, 2012(14):3438-3441.

10. Xiumei Ni, Lixi Yue, Jing Li, ZhenmingChi, Zhiqiang Liu, Madzak Catherine. Properties of alkaline proteasegenetically engineered on cell surface of the yeast Yarrowia lipolytica. Indianjournal of biochemistry & biophysics, 2009(46):294-298. SCI

11. Weilin Wang, Zhenming Chi, Zhe Chi,Jing Li, Xianghong Wang. Siderophore production by the marine-derivedAureobasidium pullulans and its antimicrobial activity. Bioresource technology.2009(100):2639-2641. SCI

12. Fang Wang, Lixi Yue, Lin Wang,Catherine Madzak, Jing Li, Xianghong Wang, Zhenming Chi. Genetic modificationof the marine-derived yeast Yarrowia lipolytica with high-protein content usinga GPI-anchor-fusion expression system. Biotechnology progress.2009(25):1297-1303. SCI

13. Zhengming Chi, Jing Li, Lin Wang, SusuHe, Xianghong Wang. Pichia pastoris INO1 gene expression affects intracellularmaltase activity and MAL1+ gene expression in Schizosaccharomyces pombe. IndianJournal of Biochemistry and Biophysics, 2008(45): 174-178. SCI

14. Lixi Yue, Zhenming Chi, Lin Wang, JiaLiu, Catherine Madzak, Jing Li, Xianghong Wang. Construction of a new plasmidfor surface display on cells of Yarrowia lipolytica. Journal of MicrobiologicalMethods, 2008(72):116-123. SCI

?? BookChapter:

Li Jing. Methods in Microbial EcologyResearch. In: Zhenming Chi, Xianghong Wang, Li Jing. ed., Microbial Ecology.Beijing, China: Science Press. 2010, pp.8-28.