薛志刚 教授
硕士招生类型：学术型
硕士招生专业：基础医学
博士招生类型：学术型
博士招生专业：基础医学
所在院系：医学院
联系方式：xuezg@tongji.edu.cn
课题组链接：https://mednew.tongji.edu.cn/ktz/Group.aspx?id=15
研究方向
主要任职
科研业绩

1、胚胎早期发育的遗传学与表观遗传学研究
2、干细胞分化表观遗传学机制及在转化医学中的应用
3、高通量测序的数据分析和生物信息学



同济大学再生医学系干细胞研究中心，同济医院干细胞临床转化中心，教授、博士生导师。



2013年，采用单细胞RNA测序技术揭示了人类和小鼠早期胚胎的遗传程序，研究成果发表在《Nature》杂志上，该研究利用单细胞表达谱研究的RNA-seq技术，突破了起始样本量少的瓶颈，对人胚胎早期发育各阶段全基因组RNA转录谱的系统分析，发现在胚胎发育早期各阶段中存在着父亲或母亲来源的单等位基因表达差异。同时运用加权基因共表达网络分析（WGCNA）显示胚胎早期发育各阶段中的细胞周期、基因调控、蛋白质翻译以及代谢通路的转录变化是以分步进行的方式按顺序发生，并明确了该机制在物种间保守存在，仅在发育特异性和时序上有所差异，证明了哺乳动物早期胚胎发育进化上的共性。此外，该研究还发现了驱动胚胎早期发育各阶段的关键候选基因。该项研究成果将在干细胞的纯化、分类及临床治疗领域产生深远的影响，也将为改善人类辅助生殖技术和提高人口出生质量提供帮助。五年内发表论文20余篇，作为课题负责人获得国家自然科学基金资助两项，多次作为科研骨干参与国家基金委、科技部973、863、国际合作等科研项目，上海市“曙光计划”项目资助。

代表性论著：
1) Xue Z*, Huang K, Cai CC, Cai LB, Jiang CY, Feng Y, Liu ZS, Zeng Q, Cheng LM, Sun YE, Liu JY*, Horvath S, Fan G*. Genetic programs in human and mouse early embryos revealed by single-cell RNA?sequencing. Nature. 500(7464): 593-597. 2013. (first author and corresponding author). IF: 38.597.
2) Huang K, Shen Y, Xue Z, Bibikova M, April C, Liu Z, Cheng L, Nagy A, Pellegrini M, Fan JB, Fan G*. A panel of CpG methylation sites distinguishes human embryonic stem cells and induced pluripotent stem cells. Stem Cell Reports. 2(1):36-43. 2013.
3) Liu H, Chen Y, Niu Y, Zhang K, Kang Y, Ge W, Liu X, Zhao E, Wang C, Lin S, Jing B, Si C, Lin Q, Chen X, Lin H, Pu X, Wang Y, Qin B, Wang F, Wang H, Si W, Zhou J, Tan T, Li T, Ji S, Xue Z, Luo Y, Cheng L, Zhou Q, Li S*, Sun YE*, Ji W*. TALEN-Mediated Gene Mutagenesis in Rhesus and Cynomolgus Monkeys. Cell Stem Cell. 14(3):323-328. 2014.
4) Chen Y, Huang K, Nakatsu MN, Xue Z*, Deng SX, Fan G*. Identification of novel molecular markers through transcriptomic analysis in human fetal and adult corneal endothelial cells. Hum Mol Genet.22(7):1271-1279. 2013. (corresponding author).
5) Hu G, Huang K, Yu J, Gopalakrishna-Pillai S, Kong J, Xu H, Liu Z, Zhang K, Xu J, Luo Y, Li S, Sun YE, Iverson LE, Xue Z*, Fan G*. Identification of miRNA signatures during the differentiation of hESCs into retinal pigment epithelial cells. PLoS One. 7(7):e37224. 2012. (corresponding author).
6) Jin L, Wu Z, Xu W, Hu X, Zhang J, Xue Z*, Cheng L*. Identifying gene expression profile of spinal cord injury in rat by bioinformatics strategy. Mol Biol Rep. 2014 Mar 5. [Epub ahead of print]
7) Liu Z, Jiang R, Yuan S, Wang N, Feng Y, Hu G, Zhu X, Huang K, Ma J, Xu G, Liu Q*, Xue Z*, Fan G*. Integrated Analysis of DNA methylation and RNA transcriptome during in vitro differentiation of human pluripotent stem cells into retinal pigment epithelial cells. PLoS One. 9(3):e91416. 2014.
8) Wang Q, Zhu XM, Feng Y, Xue Z, Fan G*. Single-cell genomics: An overview. Front. Biol. 8(6): 569-576. 2013.
9) Ge J, Ju Y, Xue Z, Feng Y, Huang X, Liu H, Zhao S*. Distal C Terminus of CaV1.2 Channels Plays a Crucial Role in the Neural Differentiation of Dental Pulp Stem Cells. PLoS One. 8(11):e81332. 2013.
10) Wu Z, Huang K, Yu J, Le T, Namihira M, Liu Y, Zhang J, Xue Z, Cheng L*, Fan G*. Dnmt3a regulates both proliferation and differentiation of mouse neural stem cells. J Neurosci Res. 90(10):1883-1891. 2012.
11) Wang A, Huang K, Shen Y, Xue Z, Cai C, Horvath S, Fan G*. Functional modules distinguish human induced pluripotent stem cells from embryonic stem cells. Stem Cells Dev. 20(11):1937-1950. 2011.
12) Xue Z, Shi Z, Dong J, Liao T, Wang Y, Sun X, Yan Z, Qian X, Cui Y, Chen J, Liu J*, Fan G*. Evaluation of x-inactivation status and cytogenetic stability of human dermal fibroblasts after long-term culture. Int J Cell Biol. 2010:289653. 2010. (first author)
13) Hutnick L, Golshani P, Namihira M, Xue Z, Matynia A, Yang X, Silva A, Schweizer F, Fan G*. DNA hypomethylation restricted to the murine forebrain induces cortical degeneration and impairs postnatal neuronal maturation. Hum Mol Genet. 18(15):2875-2888. 2009.