摘要/Abstract

摘要： 小分子泛素相关修饰物（small ubiquitin-related modifier，SUMO）化修饰在真核细胞中广泛存在，参与了多种信号转导和代谢途径。规模化鉴定SUMO化修饰蛋白质有助于从整体角度揭示SUMO化修饰的功能和作用机制。目前，有关SUMO化修饰位点的鉴定难度较大，如何规模化研究蛋白质的SUMO化修饰尚未有定论。基于此，该文系统分析了哺乳动物细胞中开展SUMO化修饰蛋白质组学的研究工作，并在总结其研究方法和成果的基础上，对SUMO的突变模式和富集方式进行详细分析，进而归纳出针对细胞和组织样品进行SUMO化修饰位点系统化鉴定的理想方法。
关键词: 小分子泛素相关修饰物, 小类泛素化修饰, 蛋白质组学, 质谱
Abstract:
Small ubiquitin-related modifier (SUMO) exists widely in all eukaryotic cells, which is involved in many signal transduction and metabolism pathways. Systematic identification of SUMOylated proteins is helpful to reveal the function and mechanism of SUMOylation from an overall perspective. At present, it is difficult to identify the SUMOylation sites, and how to study the SUMOylated proteins on a large scale has not been determined. This paper systematically analyzes the proteomic studies of protein SUMOylation in mammalian cells. On the basis of summarizing the research methods and achievements, the mutation mode and enrichment mode of SUMO are analyzed in detail, and then the ideal methods for systematic identification of SUMOylation sites in cells and tissues are concluded.
Key words: small ubiquitin-related modifier (SUMO), SUMOylation, proteomics, mass spectrometry


PDF全文下载地址:

点我下载PDF