摘要/Abstract

摘要： 目的·观察实验性牙周炎大鼠外周及中枢内炎症水平的变化。方法·通过向SD大鼠龈沟内注射牙龈卟啉单胞菌（Porphyromonas gingivalis，P. gingivalis）来源的脂多糖(lipopolysaccharide，LPS)建立实验性牙周炎模型。采用苏木精-伊红染色观察牙周组织病理变化。采用实时定量荧光 PCR评估外周血、脾脏及脑组织中白介素-1β（interleukin-1β，IL-1β)、IL-6、IL-8的表达水平。采用免疫组织化学技术观察小胶质细胞及星形胶质细胞的激活情况。组间比较采用方差分析，进一步采用Tukey法进行两两比较。结果·龈沟注射P. gingivalis-LPS可造成大鼠牙周炎样病理变化。实验性牙周炎可上调大鼠外周血、脾脏及脑内炎症因子（IL-1β、IL-6、IL-8）的表达水平，激活小胶质细胞及星形胶质细胞。结论·P. gingivalis-LPS龈沟注射法建立的实验性牙周炎可诱导外周及脑内炎症。炎症可能在牙周炎与阿尔茨海默病等系统性疾病的相关性中发挥重要作用。
关键词: 牙周炎, 牙龈卟啉单胞菌脂多糖, 外周炎症, 神经炎症
Abstract:
Objective · To observe the changes of peripheral and central inflammation in experimental periodontitis rats. Methods · An experimental periodontitis model was established by injecting lipopolysaccharide (LPS) of Porphyromonas gingivalis (P. gingivalis) into the gingival sulcus of SD rats. The pathological changes of periodontal tissue were observed with hematoxylin-eosin staining (H-E staining). The expression of interleukin-1β (IL-1β), IL-6 and IL-8 in peripheral blood, spleen and brain was evaluated by real-time PCR (RT-PCR). Immunohistochemical staining was used to observe the activation of microglia and astrocytes. Differences between groups were compared by using one-way ANOVA, and Tukey test was performed for further comparison between each 2 groups. Results · Sulcus-injection of P. gingivalis-LPS induced periodontitis-like pathologies in rats. Experimental periodontitis could increase the expression of inflammatory factors (IL-1β, IL-6, and IL-8) in the peripheral blood, spleen and brain of rats. Activation of microglial cells and astrocytes was observed in hippocampus of rats. Conclusion · The experimental periodontitis established by P. gingivalis-LPS sulcus injection could induce both peripheral and central inflammation. Inflammation could play an important role in the association between periodontitis and systemic diseases such as Alzheimer's disease.
Key words: periodontitis, P. gingivalis-LPS, peripheral inflammation, neuroinflammation


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