摘要/Abstract
摘要： 目的·研究长链非编码RNA（long noncoding RNA，lncRNA）COL11A1-208在口腔鳞状细胞癌（简称口腔鳞癌）中的表达水平，并评价其在患者预后预测和诊断中的潜在价值。方法·采用lncRNA基因表达谱芯片检测6对口腔鳞癌组织及对应癌旁正常组织中差异表达的lncRNA；采用实时荧光定量PCR（quantitative real-time PCR，qPCR）检测74对口腔鳞癌组织及癌旁正常组织中COL11A1-208的表达，并比较不同临床特征患者COL11A1-208表达水平的差异。采用受试者工作特征（receiver operating characteristic，ROC）曲线评价癌组织COL11A1-208水平在口腔鳞癌诊断中的潜能。Kaplan-Meier生存分析评估COL11A1-208表达水平对口腔鳞癌患者3年总体生存率的影响。结果·qPCR检测结果显示，COL11A1-208在74例口腔鳞癌组织中的表达显著高于癌旁正常组织（P=0.000），与lncRNA基因芯片的结果一致。结合患者临床特征分析发现，TNM分期较晚（Ⅲ～Ⅳ）的口腔鳞癌组织的COL11A1-208表达水平显著高于分期较早（Ⅰ～Ⅱ）的癌组织（P=0.001）；组织COL11A1-208水平用于诊断口腔鳞癌的ROC曲线下面积为0.702（95%CI 0.617～0.788，P=0.000），灵敏度和特异度分别为87.8%和54.1%；高表达COL11A1-208患者3年生存率显著低于低表达者（P=0.002）。结论·COL11A1-208在口腔鳞癌组织内高表达，在TNM分期较晚的癌组织表达水平更高；COL11A1-208可能在口腔鳞癌预后预测及诊断中有潜在应用价值。
关键词: 口腔鳞状细胞癌, 长链非编码RNA, COL11A1-208, 预后, 诊断
Abstract:
Objective · To investigate the expression of long noncoding RNA （lncRNA） COL11A1-208 in oral squamous cell carcinoma （OSCC）, and evaluate its potential value in prognosis prediction and diagnosis of OSCC. Methods · The differentially expressed lncRNAs from six pairs of OSCC tissues and the para-carcinoma tissues were detected by lncRNA microarray. Quantitative real-time PCR （qPCR） was used to detect the expression of COL11A1-208 in seventy-four pairs of OSCC tissues and para-carcinoma tissues. The difference of COL11A1-208 expression among the patients with different clinical features were analyzed. The potential effectiveness of COL11A1-208 level in the diagnosis of OSCC was evaluated by the receiver operating characteristic （ROC） curve. Kaplan-Meier survival analysis was used to analyze the effects of COL11A1-208 on the 3-year overall survival rate of OSCC patients. Results · Compared with the para-carcinoma tissues, COL11A1-208 was highly expressed in OSCC tissues （P=0.000）, which was consistent with the results of lncRNA microarray. The expression of COL11A1-208 in the patients with advanced TNM stage （Ⅲ - Ⅳ） was significantly higher than that with TNM stage Ⅰ - Ⅱ （P=0.001）. The area under ROC curve of COL11A1-208 as a biomarker for the diagnosis of OSCC was 0.702 （95%CI 0.617 -0.788, P=0.000）, and the sensitivity and specificity were 87.8% and 54.1%, respectively. The 3-year overall survival rate of the patients with low expression of COL11A1-208 was significantly longer than that of the patients with high expression （P=0.002）. Conclusion · The expression of COL11A1-208 is up-regulated in OSCC tissues, which is even higher in the patients with advanced TNM stage. COL11A1-208 might have potential value in prognostic prediction and diagnosis of OSCC.
Key words: oral squamous cell carcinoma （OSCC）, long noncoding RNA （lncRNA）, COL11A1-208, prognosis, diagnosis
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