摘要/Abstract
摘要： 目的·分析lnc-MTBP-5在结直肠癌中的表达，并探讨其对结直肠癌细胞侵袭能力的影响及可能的作用机制。方法·从SRA（Sequence ReadArchive）数据库提取PRJNA218851和PRJNA376161数据集的生物信息学资料，筛选与结直肠癌转移相关的lncRNA。在癌症基因组图谱（TCGA）中分析lnc-MTBP-5在结直肠癌和正常组织中的表达差异，与患者预后的关系，及与转移相关因子的相关性。采用实时荧光定量PCR检测lnc-MTBP-5在正常肠上皮细胞和结直肠癌细胞、53例患者的结直肠癌组织及癌旁组织中的表达量。在结直肠癌细胞中下调lnc-MTBP-5后，行CCK-8实验、克隆形成实验和侵袭实验，观察lnc-MTBP-5对结直肠癌细胞增殖和侵袭功能的影响。结果·lnc-MTBP-5和结直肠癌转移相关，且lnc-MTBP-5在5种结直肠癌细胞和结直肠癌组织中均呈高表达。与低表达患者相比，高表达lnc-MTBP-5的结直肠癌患者年龄小、美国癌症联合委员会（AJCC）分期高，且易发生转移。lnc-MTBP-5与结肠癌转移相关因子1（metastasis associated in colon cancer 1，MACC1）、间质上皮转换因子（mesenchymal to epithelial transition factor，MET）、钙黏着蛋白关联蛋白呈正相关。下调lnc-MTBP-5后，结直肠癌细胞的侵袭能力减弱。结论·lnc-MTBP-5在结直肠癌细胞系及结直肠癌组织中表达显著增高，且与患者预后呈负相关。lnc-MTBP-5增强结直肠癌细胞的侵袭能力，该功能可能与MACC1-HGF（hepatocyte growth factor，肝细胞生长因子）/MET通路有关。
关键词: lnc-MTBP-5, 结直肠癌, 细胞侵袭, 细胞增殖
Abstract:
Objective · To analyze the expression of lnc-MTBP-5 in colorectal cancer (CRC), and explore the effect of lnc-MTBP-5 on the invasion of CRC cells and its potential mechanism. Methods · Bioinformatics data from PRJNA218851 and PRJNA376161 data sets were extracted from the Sequence ReadArchive (SRA) database to screen CRC metastasis associated lncRNAs. The Cancer Genome Atlas (TCGA) was used to analyze the expression of lnc-MTBP-5 in CRC tissues and normal tissues, its relationship with the prognosis of patients, and its correlation with metastasis related factors. Quantitative real-time PCR (qPCR) was used to detect the expression of lnc-MTBP-5 in normal intestinal epithelial cells and CRC cells, as well as 53 CRC tissues and para-cancer mucosa. After lnc-MTBP-5 was down-regulated in CRC cells, CCK-8 assay, clone formation and Transwell assay were performed to observe the effect of lnc-MTBP-5 on the proliferation and invasion ability of CRC cells. Results · Lnc-MTBP-5 was associated with CRC metastasis. The expression of lnc-MTBP-5 was significantly increased in 5 CRC cell lines and CRC tissues. Compared with patients with low expression of lnc-MTBP-5, CRC patients with high expression of lnc-MTBP-5 were younger, had higher American Joint Committee on cancer (AJCC) staging, and were prone to metastasis. Lnc-MTBP-5 was positively correlated with CRC metastasis associated in colon cancer 1 (MACC1), mesenchymal to epithelial transition factor (MET) and cadherin-associated protein. After lnc-MTBP-5 was down-regulated, the invasion ability of CRC cells decreased. Conclusion · Lnc-MTBP-5 is up-regulated in CRC cell lines and CRC tissues, and it is negatively correlated with the prognosis of patients. Lnc-MTBP-5 can promote the invasion ability of CRC cells, which may be related to MACC1-HGF (hepatocyte growth factor)/MET pathway.
Key words: lnc-MTBP-5, colorectal cancer (CRC), cell invasion, cell proliferation
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