摘要/Abstract
摘要： 目的·探讨原发性干燥综合征（primary Sj?gren's syndrome， pSS）患者唇腺组织中水通道蛋白5（aquaporin 5， AQP5）、血清白细胞介素-21（interleukin-21， IL-21）、β2微球蛋白（β2-microglobulin， β2-MG）的表达水平与病情活动之间的关系。方法·收集39例pSS患者的病例资料，检测红细胞沉降率（erythrocyte sedimentation rate， ESR）和免疫球蛋白G（immunoglobulin， IgG）等炎症指标。行唇腺活检，根据Chisholm唇腺病理进行分级，将所有标本分为Ⅰ、Ⅱ、Ⅲ、Ⅳ4组。免疫组织化学法检测唇腺组织AQP5的表达，ELISA法检测血清IL-21、β2-MG水平。结果·随着唇腺组织淋巴细胞数目的增加，AQP5蛋白表达量减少。唇腺组织AQP5的表达与IL-21、β2-MG、ESR、IgG呈负相关（r=-0.924， P=0.000； r=-0.366， P=0.022； r=-0.611，P=0.000；r=-0.741， P=0.000）。随着唇腺组织淋巴细胞数目的增加，血清IL-21表达增加，4组间IL-21的表达差异有统计学意义。血清β2-MG在各组间未见明显差异。结论·pSS患者唇腺组织AQP5的表达与淋巴细胞浸润有关，且AQP5、IL-21、β2-MG与病情活动度存在相关性，可作为生物学标志物，用于临床病情的评估。
关键词: 原发性干燥综合征, 水通道蛋白5, 白细胞介素-21, β2微球蛋白
Abstract:
Objective · To investigate the expression level of aquaporin 5 (AQP5) in labial gland, and interleukin-21 (IL-21) and β2-microglobulin (β2-MG) in serum of patients with primary Sj?gren's syndrome (pSS) and their association with the activity of the disease. Methods · Thirty-nine patients with pSS were collected and inflammatory indicators such as erythrocyte sedimentation rate (ESR) and IgG were detected. Lip gland biopsy was performed. All specimens were divided into four groups according to Chisholm lip gland pathological classification:Ⅰ, Ⅱ, Ⅲ and Ⅳ. The expression of AQP5 in labial gland was detected by immunohistochemistry and the levels of IL-21 and β2-MG in serum were detected by ELISA. Results · As the number of lymphocytes in labial gland increased, the expression of AQP5 protein decreased. The expression of AQP5 in labial gland was negatively correlated with IL-21, β2-MG, ESR and IgG (r=-0.924, P=0.000; r=-0.366, P=0.022; r=-0.611, P=0.000; r=-0.741, P=0.000). As the number of lymphocytes in labial gland increased, the expression of IL-21 in serum increased, and there was significant difference among four grades. There was no significant difference in serum β2-MG among the groups. Conclusion · The expression of AQP5 in labial gland of patients with pSS is related to lymphocyte infiltration, and AQP5, IL-21 and β2-MG are related to disease activity, which can be used as biological markers for clinical disease evaluation.
Key words: primary Sj?gren's syndrome (pSS), aquaporin 5 (AQP5), interleukin-21 (IL-21), β2-microglobulin (β2-MG)
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