摘要/Abstract
摘要： 目的·研究肥胖和肥胖抵抗大鼠在正畸牙移动速率和压力侧骨改建中的差异。方法·将40只SD雄性大鼠随机分为普通饲料（normal diet，ND）组和高脂饲料(high-fat diet，HFD)组，HFD组后续分为肥胖(diet-induced obese，DIO)组和肥胖抵抗(diet-resistant，DR)组各10只。分别在大鼠上颌左侧安装加力装置，于加力14 d后处死大鼠并收集大鼠双侧上颌磨牙及其周围牙槽骨。采用抗酒石酸酸性磷酸酶染色和免疫组化染色观察3组大鼠压力侧破骨和成骨活性的改变，Micro-CT测量分析牙移动距离和压力侧骨参数的变化。结果·DR组的牙移动速率明显慢于DIO组和ND组（P=0.000，P=0.005）。加力14 d后，DR组的骨参数如骨密度、骨体积分数、骨小梁数和骨小梁宽度均明显高于DIO组（P=0.001，P=0.000，P=0.005，P=0.000）。DR组的破骨细胞数明显低于ND组和DIO组（P=0.005，P=0.001）。DIO组骨钙蛋白的表达明显多于ND组（P=0.047）。结论·高脂饮食诱导的肥胖或者肥胖抵抗可能通过促进或抑制破骨细胞生成影响正畸牙移动压力侧骨改建过程，从而影响牙移动速率。
关键词: 肥胖, 肥胖抵抗, 微计算机断层扫描技术（Micro-CT）, 正畸牙移动, 破骨细胞, 大鼠
Abstract:
Objective · To investigate on the difference of the orthodontic tooth movement rate and pressure side bone remodeling between diet-induced obese rats and diet-resistant rats. Methods · Forty male Sprague-Dawley rats were randomly divided into two groups, including the normal diet (ND) group and high-fat diet (HFD) group. And then the HFD group was subdivided into diet-induced obese (DIO) group (n=10) and diet-resistant (DR) group (n=10). Orthodontic applications were placed between the left upper first molar and incisors in rats respectively. After orthodontic treatment for 14 d, the rats were executed and their maxillae were harvested bilaterally. The number of osteoclasts in 3 groups were observed by tartrate-resistant acid phosphatase (TRAP) staining. The expression of osteocalcin in 3 groups was observed by immunohistochemical staining. The tooth movement distance and trabecular bone parameters on the pressure side were measured by Micro-CT. Results · The rate of orthodontic tooth movement was significantly lower in the DR group than that in the DIO and ND groups (P=0.000, P=0.005). After orthodontic treatment for 14 d, the bone mineral density, percentage trabecular bone volume/total volume, trabecular number and trabecular thickness in the DR group were significantly higher than those in the DIO group (P=0.001, P=0.000, P=0.005, P=0.000). The number of osteoclasts in the DR group was significantly lower than that in the ND group and DIO group (P=0.005, P=0.001). The expression of osteocalcin was higher in the DIO group than that in the ND group (P=0.047). Conclusion · High-fat diet-induced obesity or obesity-resistance may affect bone remodeling process during orthodontic treatment by promoting or inhibiting osteoclastogenesis, thereby affecting the rate of tooth movement.
Key words: obesity, obesity resistance, Micro-CT, orthodontic tooth movement, osteoclast, rat
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