摘要/Abstract
摘要： 目的·探讨以浓缩生长因子（concentrated growth factor，CGF）为支架，联合基质细胞衍生因子1（stromal cell-derived factor-1，SDF-1）应用于年轻恒牙牙髓再生的可行性。方法·体外分离培养犬牙髓细胞（dental pulp cells，DPC），观察SDF-1和CGF对其增殖、分化的影响；建立比格犬年轻恒切牙无髓模型，分为天然牙髓组（A组）、空管组（B组）、填充CGF组（C组）和复合SDF-1/CGF组（D组），10周后行影像学检查、RT-PCR及组织学观察。结果·CGF浸提液（concentrated growth factor extraction，CGFe）促进DPC体外增殖，联合SDF-1可加强其促增殖作用，且可促进细胞成牙、成血管分化；影像学检查示4组根尖均有不同程度生长；RT-PCR示D组新生组织中成牙、成血管相关基因表达均高于C组；组织学观察示C组和D组产生新生牙本质及类牙髓样组织，而B组仅在根尖部产生类牙骨质。结论·CGF可作为年轻恒牙牙髓样组织再生的有效支架，与SDF-1联合应用可促进这一过程。
关键词: 年轻恒牙, 牙髓再生, 基质细胞衍生生长因子, 浓缩生长因子
Abstract:
Objective · To evaluate the feasibility of immature permanent teeth pulp regeneration with a new method that utilizes the integration of concentrated growth factor (CGF) as a scaffold and stromal cell-derived factor-1 (SDF-1). Methods · Canine dental pulp cells (DPC) were isolated and cultured in vitro. Then the effects of SDF-1 and CGF were observed on DPC proliferation and differentiation. The pulpless model was established on the beagle’s immature incisors which were divided into four groups: natural pulp (A), empty cannel (B), CGF-filling (C) and SDF-1/CGF-filling (D). After 10 weeks, specimens were checkedimaging examination, RT-PCR and histological observation. Results · CGF extraction (CGFe) induced DPC proliferation while the combination of SDF-1 and CGFe enhanced this effect and also facilitated odontogenic and angiogenic differentiation of DPC. According to imaging examination, the apex growth of all four groups was in varying degrees. RT-PCR indicated the s of odontogenesis and angiogenesis related genes in group D were higher than those in group C. Besides, neonatal dentin and dental-pulp-like tissue were observed inside the canal of both group C and D, while only cementum-like tissue existed around root apex of group B. Conclusion · SDF-1 plays an important role in driving the process of pulp-like-tissue regeneration of immature permanent teethusing CGF as an effective scaffold.
Key words: immature permanent teeth, pulp regeneration, stromal cell-derived factor-1 (SDF-1), concentrated growth factor (CGF)
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