摘要/Abstract

摘要： 目的·研究胃癌组织细胞中长链非编码RNA（long-chain non-coding RNA，lncRNA）lincRNA-BBOX1-2的表达对于胃癌诊断及预后判断的价值。方法·实时定量PCR检测45例胃癌组织及癌旁正常组织中lincRNA-BBOX1-2的表达水平，分析lincRNA-BBOX1-2的表达水平与胃癌患者临床病理特征及预后的关系。结果·胃癌组织中lincRNA-BBOX1-2的表达水平高于癌旁正常组织 （3.291±0.274 vs 1.125±0.075，P0.000）；胃癌组织中lincRNA-BBOX1-2的表达水平与肿瘤有/无淋巴结转移（P0.005，r0.172）及 TNM 分期（P0.013，r0.137）呈正相关。受试者工作特征曲线分析结果显示：lincRNA-BBOX1-2在预测胃癌发生时，其曲线下面积（area under the curve，AUC）值为0.916（95% CI 0.859～0.972）；lincRNA-BBOX1-2在预测胃癌有/无淋巴结转移时，其 AUC值为0.720 （95% CI 0.565～0.875）。结论·胃癌组织中lincRNA-BBOX1-2的表达水平上调与胃癌的临床病理特征密切相关。lincRNA-BBOX1-2可能成为一个新的胃癌诊断分子标志物。
关键词: 长链非编码RNA, lincRNA-BBOX1-2, 胃癌, TNM分期
Abstract:
Objective · To evaluate the of long-chain non-coding RNA (lncRNA) lincRNA-BBOX1-2 in gastric cancer tissues and the value of diagnosis and prognosis of gastric cancer. Methods · The of lincRNA-BBOX1-2 was detectedreal-time quantitative PCR in 45 cases of gastric cancer and adjacent normal tissues. The correlations of lincRNA-BBOX1-2 with clinic-pathological features and clinical prognosis were analyzed. Results · The higher of lincRNA-BBOX1-2 was observed in the gastric cancer tissues (3.291±0.274 vs 1.125±0.075, P0.000) as compared with the adjacent normal tissues. Up-regulation of lincRNA-BBOX1-2 was associated with positive lymph node metastasis (P0.005, r0.172) and advanced clinical stage (P0.013, r0.137). Receiver operating characteristic curve analysis showed that the area under the curve (AUC) value was 0.916 (95%CI 0.859–0.972) for lincRNA-BBOX1-2 in predicting the occurrence of gastric cancer, and 0.720 (95%CI 0.565–0.875) for lincRNA-BBOX1-2 in predicting the lymph node metastasis. Conclusion · The up-regulation of lincRNA-BBOX1-2 in gastric cancer tissues is associated with the clinic-pathological features of gastric cancer. lincRNA-BBOX1-2 may be used as a potential diagnostic biomarker in patients with gastric cancer.
Key words: long-chain non-coding RNA (lncRNA), lincRNA-BBOX1-2, gastric cancer, TNM stage


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