摘要/Abstract
摘要： 目的 ·构建一种携载抗菌黏性脂质体的水凝胶（ A-LIP-PEG），探究其抗菌及促进骨修复作用。方法 ·基于脂质体和多巴胺的席夫碱反应，制备黏性脂质体（ A-LIP）。将 A-LIP和巯基化的聚乙二醇（ 4SH-PEG）混合，用 Ag+交联 4SH-PEG制备复合脂质体水凝胶。通过 A-LIP对骨形态发生蛋白 2（bone morphogenetic protein 2，BMP-2）的包载，实现 BMP-2在水凝胶网络中均匀分散和缓慢释放。采用粒度仪考察 A-LIP的粒径和电位；采用透射电镜观察 A-LIP的形态；采用冲洗黏附实验评估 A-LIP的黏性；通过 CCK-8验证 A-LIP-PEG的生物相容性；通过骨髓间充质干细胞的碱性磷酸酶测定及茜素红 S染色评定其促成骨活性。结果 ·体外研究表明， A-LIP对软组织有很强的黏附性； A-LIP-PEG对金黄色葡萄球菌有明显的抑制作用； A-LIP-PEG具有较脂质体水凝胶（ LIP-PEG）更显著的促进成骨分化作用，且不影响细胞的增殖。结论 ·该 A-LIP-PEG在拓宽水凝胶装载和控释药物及抗菌、骨修复方面有良好的前景。
关键词: 黏性脂质体, 抗菌, 水凝胶, 骨修复
Abstract:
Objective · To construct an antibacterial hydrogel laden with adhesive liposomes (A-LIP-PEG) for bone repair. Methods · Adhesive liposomes (A-LIP) were fabricated based on the Schiff-based reaction between dopamine and the liposome. To prepare the liposome composite hydrogel, the A-LIPs were mixed with thiolated polyethylene glycol (4SH-PEG) which was then crosslinked with Ag+. Bone morphogenetic protein 2 (BMP-2) was loaded in the A-LIPs for its uniform dispersion and sustained release in the hydrogel network. The size distribution and zeta-potential of the A-LIPs were characterized with a particle size analyzer. The morphology of the A-LIPs was observed under transmission electron microscope. Flushing test was employed to examine the viscidity of the A-LIPs, and CCK-8 assay was conducted to demonstrate the biocompatibility of the A-LIP-PEG. Osteogenic activity of the A-LIP-PEG was evaluatedalkaline phosphatase assay and alizarin red S staining in bone marrow mesenchymal stem cells (BMSCs). Results · Strong adhesion toward soft tissue of the A-LIPs was indicatedin vitro study. A-LIP-PEG showed significant inhibition on Staphylococcus aureus. A-LIP-PEG showed superior promotion of osteogenic differentiation to hydrogel laden with liposomes (LIP-PEG), without disrupting cell proliferation. Conclusion · The A-LIP-PEG developed in this study shows nopotential to expand the application of hydrogels to drug delivery, antibacteria and bone repair.
Key words: adhesive liposome, antibacteria, hydrogels, bone repair
PDF全文下载地址:
点我下载PDF
