摘要/Abstract

摘要： 目的 ·初步探究 640 nm红光促进角质形成细胞迁移的机制。方法 ·体外培养人角质形成细胞，根据干预方式不同分为 4组：对照组、 sitagliptin组（抑制 CD26表达）、640 nm红光组（ 8 J/cm2）、640 nm红光 +sitagliptin组。Realtime-PCR检测角质形成细胞中 CD26 mRNA的表达水平， Western blotting检测 CD26蛋白表达情况， Transwell和细胞划痕实验观察角质形成细胞的迁移能力。结果 · Realtime-PCR和 Western blotting结果显示， 640 nm红光组与对照组相比 CD26 mRNA和蛋白表达有所升高，而 sitagliptin组明显低于对照组（均 P<0.05）；Transwell和细胞划痕实验均可观察到 640 nm红光组角质形成细胞迁移能力增强，而 sitagliptin组迁移能力下降。结论 · 640 nm红光照射可促使 CD26表达增加，加快角质形成细胞迁移，从而缩短再上皮化进程。
关键词: 640 nm红光, CD26, 角质形成细胞, 创面修复, 再上皮化
Abstract:
Objective · To investigate the mechanism of 640 nm red light promoting keratinocyte migration preliminarily. Methods · Human keratinocytes were cultured in vitro and assigned to 4 groups according to different interventions, i.e. control group, sitagliptin group (CD26 inhibited), 640 nm red light group (8 J/cm2) and 640 nm red light+sitagliptin group. The levels of CD26 mRNA and protein in keratinocytes were measured with realtime-PCR and Western blotting, respectively. Cell migration was observed with Transwell assay and scratch assay. Results · The level of CD26 mRNA and the of CD26 protein in 640 nm red light group obviously increased compared with control group, while those of sitagliptin group significantly decreased (all P<0.05). Migration ability of keratinocytes increased in 640 nm red light group and decreased in sitagliptin group observedTranswell assay and scratch assay. Conclusion · The of CD26 in keratinocytes is promotedthe 640 nm red light, which can accelerate keratinocyte migration to facilitate re-epithelialization.
Key words: 640 nm red light, CD26, keratinocyte, wound healing, re-epithelialization


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