摘要/Abstract

摘要： 目的 ·探讨小鼠睾丸中 SUMO特异肽酶 3（SUMO specific peptidase 3，SENP3，又称 SUMO特异蛋白酶 3）对自噬的调控作用。方法 ·采用免疫荧光法对 SENP3在睾丸生精细胞和支持细胞（ Sertoli cells，简称 Sertoli细胞）中的定位进行检测。对 Senp3野生型（ Senp3+/+）小鼠和 Senp3基因敲除杂合子（ Senp3+/-）小鼠进行饥饿处理，诱导自噬的发生。提取小鼠的睾丸组织蛋白质，采用 Western blotting检测自噬的程度。利用透射电子显微镜技术、免疫荧光法检测睾丸组织切片中的细胞自噬，并区分睾丸生精细胞和 Sertoli细胞自噬的程度。结果 · SENP3主要定位于 Sertoli细胞核。与 Senp3+/+小鼠相比， Senp3+/-小鼠饥饿后， Sertoli细胞自噬增加。结论 · SENP3能够抑制营养缺乏时 Sertoli细胞自噬，可能对细胞自噬的程度发挥控制作用。
关键词: 自噬, SUMO特异蛋白酶 3, 睾丸, 支持细胞（ Sertoli细胞）
Abstract:
Objective · To investigate the regulation of autophagySUMO specific peptidase 3 (SENP3, normally called SUMO specific protease 3) in motestis. Methods · Immunofluorescence was used to detect the localization of SENP3 in spermatogenic cells and Sertoli cells of testis. Senp3 wild type (Senp3+/+) mice and Senp3 gene knockout heterozygous (Senp3+/-) mice were subjected to starvation treatment to induce autophagy. Testicular tissue proteins were extracted, and the extent of autophagy was detectedWestern blotting. The extent of autophagy of Sertoli cells was detected and compared with that of spermatogenic cells in testistransmission electron microscopy and immunofluorescence. Results · SENP3 mainly localized in the nucleus of Sertoli cells. Compared to Senp3+/+ mice, the extent of starvation-induced autophagy in Sertoli cells of Senp3+/- mice increased. Conclusion · SENP3 can inhibit autophagy in Sertoli cells during nutrient deficiency, which may play a role in controlling the extent of autophagy.
Key words: autophagy, SUMO specific protease 3, testis, Sertoli cell


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